CNOT7

CCR4-NOT transcription complex subunit 7 is a protein that in humans is encoded by the CNOT7 gene. It is a subunit of the CCR4-Not deadenylase complex.

CNOT7
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2D5R, 4GMJ
Identifiers
Aliases	CNOT7, CAF1, Caf1a, hCAF-1, CAF-1, CCR4-NOT transcription complex subunit 7
External IDs	OMIM: 604913; MGI: 1298230; HomoloGene: 49011; GeneCards: CNOT7; OMA:CNOT7 - orthologs
Gene location (Human) Chr. Chromosome 8 (human) Band 8p22 Start 17,224,966 bp End 17,246,878 bp
Gene location (Mouse) Chr. Chromosome 8 (mouse) Band 8|8 A4 Start 40,945,581 bp End 40,968,888 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in oocyte buccal mucosa cell corpus epididymis secondary oocyte germinal epithelium Skeletal muscle tissue of biceps brachii caput epididymis parotid gland Skeletal muscle tissue of rectus abdominis tail of epididymis Top expressed in superior cervical ganglion cumulus cell medial ganglionic eminence tail of embryo secondary oocyte genital tubercle medullary collecting duct dorsal striatum ureter abdominal wall More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nuclease activity nucleic acid binding exoribonuclease activity protein binding 3'-5'-exoribonuclease activity signal transducer activity poly(A)-specific ribonuclease activity exonuclease activity RNA binding DNA-binding transcription factor activity hydrolase activity metal ion binding transcription factor binding transcription corepressor activity Cellular component nucleus CCR4-NOT complex cytosol membrane nuclear body P-body cytoplasm nuclear speck CCR4-NOT core complex host cell PML body Biological process negative regulation of cell population proliferation negative regulation of gene expression exonucleolytic catabolism of deadenylated mRNA deadenylation-dependent decapping of nuclear-transcribed mRNA nucleic acid phosphodiester bond hydrolysis P-body assembly positive regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay regulation of transcription, DNA-templated positive regulation of cell population proliferation positive regulation of nuclear-transcribed mRNA poly(A) tail shortening positive regulation of transcription by RNA polymerase II positive regulation of mRNA catabolic process gene silencing by miRNA regulation of translation nuclear-transcribed mRNA poly(A) tail shortening signal transduction gene silencing transcription, DNA-templated RNA phosphodiester bond hydrolysis, exonucleolytic DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest negative regulation of transcription by RNA polymerase II regulation of tyrosine phosphorylation of STAT protein positive regulation of viral genome replication negative regulation of transcription, DNA-templated defense response to virus negative regulation of type I interferon-mediated signaling pathway carbohydrate metabolic process negative regulation of translation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 29883 18983 Ensembl ENSG00000198791 ENSMUSG00000031601 UniProt Q9UIV1 Q60809 RefSeq (mRNA) NM_013354 NM_054026 NM_001322087 NM_001322088 NM_001322089 NM_001322090 NM_001322091 NM_001322092 NM_001322093 NM_001322094 NM_001322095 NM_001322096 NM_001322097 NM_001322098 NM_001322099 NM_001322100 NM_001271542 NM_001271543 NM_011135 NM_001361938 NM_001361939 RefSeq (protein) NP_001309016 NP_001309017 NP_001309018 NP_001309019 NP_001309020 NP_001309021 NP_001309022 NP_001309023 NP_001309024 NP_001309025 NP_001309026 NP_001309027 NP_001309028 NP_001309029 NP_037486 NP_473367 NP_001258471 NP_001258472 NP_035265 NP_001348867 NP_001348868 Location (UCSC) Chr 8: 17.22 – 17.25 Mb Chr 8: 40.95 – 40.97 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene binds to an anti-proliferative protein, B-cell translocation protein 1, which negatively regulates cell proliferation. Binding of the two proteins, which is driven by phosphorylation of the anti-proliferative protein, causes signaling events in cell division that lead to changes in cell proliferation associated with cell-cell contact. The protein has both mouse and yeast orthologs. Alternate splicing of this gene results in two transcript variants encoding different isoforms.

Interactions

CNOT7 has been shown to interact with:

• BTG1,
• PABPC1,
• TOB1, and
• TOB2.

Further reading

• Draper MP, Salvadore C, Denis CL (1995). "Identification of a mouse protein whose homolog in Saccharomyces cerevisiae is a component of the CCR4 transcriptional regulatory complex". Mol. Cell. Biol. 15 (7): 3487–95. doi:10.1128/MCB.15.7.3487. PMC 230585. PMID 7791755.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Hillier LD, Lennon G, Becker M, Bonaldo MF, Chiapelli B, Chissoe S, Dietrich N, DuBuque T, Favello A, Gish W, Hawkins M, Hultman M, Kucaba T, Lacy M, Le M, Le N, Mardis E, Moore B, Morris M, Parsons J, Prange C, Rifkin L, Rohlfing T, Schellenberg K, Bento Soares M, Tan F, Thierry-Meg J, Trevaskis E, Underwood K, Wohldman P, Waterston R, Wilson R, Marra M (1996). "Generation and analysis of 280,000 human expressed sequence tags". Genome Res. 6 (9): 807–28. doi:10.1101/gr.6.9.807. PMID 8889549.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, Suyama A, Sugano S (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Rouault JP, Prévôt D, Berthet C, Birot AM, Billaud M, Magaud JP, Corbo L (1998). "Interaction of BTG1 and p53-regulated BTG2 gene products with mCaf1, the murine homolog of a component of the yeast CCR4 transcriptional regulatory complex". J. Biol. Chem. 273 (35): 22563–9. doi:10.1074/jbc.273.35.22563. PMID 9712883.
• Bogdan JA, Adams-Burton C, Pedicord DL, Sukovich DA, Benfield PA, Corjay MH, Stoltenborg JK, Dicker IB (1998). "Human carbon catabolite repressor protein (CCR4)-associative factor 1: cloning, expression and characterization of its interaction with the B-cell translocation protein BTG1". Biochem. J. 336 (2): 471–81. doi:10.1042/bj3360471. PMC 1219893. PMID 9820826.
• Ikematsu N, Yoshida Y, Kawamura-Tsuzuku J, Ohsugi M, Onda M, Hirai M, Fujimoto J, Yamamoto T (1999). "Tob2, a novel anti-proliferative Tob/BTG1 family member, associates with a component of the CCR4 transcriptional regulatory complex capable of binding cyclin-dependent kinases". Oncogene. 18 (52): 7432–41. doi:10.1038/sj.onc.1203193. PMID 10602502.
• Prévôt D, Morel AP, Voeltzel T, Rostan MC, Rimokh R, Magaud JP, Corbo L (2001). "Relationships of the antiproliferative proteins BTG1 and BTG2 with CAF1, the human homolog of a component of the yeast CCR4 transcriptional complex: involvement in estrogen receptor alpha signaling pathway". J. Biol. Chem. 276 (13): 9640–8. doi:10.1074/jbc.M008201200. PMID 11136725.
• Yoshida Y, Hosoda E, Nakamura T, Yamamoto T (2001). "Association of ANA, a member of the antiproliferative Tob family proteins, with a Caf1 component of the CCR4 transcriptional regulatory complex". Jpn. J. Cancer Res. 92 (6): 592–6. doi:10.1111/j.1349-7006.2001.tb01135.x. PMC 5926753. PMID 11429045.
• Flanagan J, Healey S, Young J, Whitehall V, Chenevix-Trench G (2003). "Analysis of the transcription regulator, CNOT7, as a candidate chromosome 8 tumor suppressor gene in colorectal cancer". Int. J. Cancer. 106 (4): 505–9. doi:10.1002/ijc.11264. PMID 12845644. S2CID 25191544.
• Rual JF, Venkatesan K, Hao T, Hirozane-Kishikawa T, Dricot A, Li N, Berriz GF, Gibbons FD, Dreze M, Ayivi-Guedehoussou N, Klitgord N, Simon C, Boxem M, Milstein S, Rosenberg J, Goldberg DS, Zhang LV, Wong SL, Franklin G, Li S, Albala JS, Lim J, Fraughton C, Llamosas E, Cevik S, Bex C, Lamesch P, Sikorski RS, Vandenhaute J, Zoghbi HY, Smolyar A, Bosak S, Sequerra R, Doucette-Stamm L, Cusick ME, Hill DE, Roth FP, Vidal M (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Res. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.

External links

• Human CNOT7 genome location and CNOT7 gene details page in the UCSC Genome Browser.
• Overview of all the structural information available in the PDB for UniProt: Q9UIV1 (CCR4-NOT transcription complex subunit 7) at the PDBe-KB.