Coronin-6 also known as coronin-like protein E (Clipin-E) is a protein that in humans is encoded by the CORO6 gene.
| CORO6 | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| Identifiers | |||||||||||||||||||||||||||||||||||||||||||||||||||
| Aliases | CORO6, coronin 6 | ||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | MGI: 2183448; HomoloGene: 104099; GeneCards: CORO6; OMA:CORO6 - orthologs | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Coronin-6 belongs to the coronin family which is an actin binding protein.[5][6] Human CORO6 gene is located on chromosome 17 on the cytogenetic band 17 p11.2.[7] Gene CORO6 is well conserved across domain of eukaryote organisms from fungus to animal.[8]
Expression
editEST profile
editBased on the EST profile, CORO6 expressed in high level at the larynx, nerve and muscle. CORO6 has also been shown to be expressed in high levels in the breast (mammary gland) tumor. During the human development stage, the higher level of CORO6 expressed at blastocyst and adult.[9]
Transcript Variant
editAlternative mRNAs are shown aligned from 5' to 3' on a virtual genome where introns have been shrunk to a minimal length. Exon size is proportional to length, intron height reflects the number of cDNAs supporting each intron. Introns of the same color are identical, of different colors are different. 'Good proteins' are pink, partial or not-good proteins are yellow, uORFs are green. 5' cap or3' poly A flags show completeness of the transcript . CORO6 contains 21 distinct gt-ag introns. Transcription produces 10 alternatively spliced mRNA. There are 3 probable alternative promoters, and validated alternative polyadenylation sites.[10]
Structure
editCORO6 protein sequence contains WD-40 repeats. WD40 domain is a structural motif found in Eukaryotes and cover variety of functions, such as adaptor or regulatory modules in signal transduction, pre-mRNA processing and cytoskeletal assembly. It usually terminating at WD dipeptide at its C-terminus and is about 40 residues long, so called WD40.[11]
The structure of CORO6 is predicted by using Phyre2 program. It is similar to the crystal structure of murine coronin-1. 390 residues ( 83% of CORO6 protein sequence) have been modelled with 100.0% confidence by the single highest scoring template. Image coloured by rainbow N → C terminus
Homology
editParalogs
editHuman proteins which are the paralogs to CORO6, CORO1A, CORO1B, CORO1C, CORO2A, CORO2B, CORO7
The table compared Homo sapiens protein CORO6 to its paralogs
| Name of paralogs | CORO6 | CORO1A | CORO1B | CORO2A | CORO2B | CORO7 |
|---|---|---|---|---|---|---|
| Accession number | NP_116243 | NP_009005 | NP_065174 | NP_438171 | NP_006082 | NP_078811 |
| Sequence length | 472 aa | 461 aa | 489 aa | 525 aa | 480 aa | 925 aa |
| Sequence identity | 67% | 67% | 45% | 45% | 32% | |
| Sequence similarity | 81% | 80% | 64% | 63% | 49% |
By comparing its paralogs we found that CORO1A and CORO1B are most related to CORO6.
Orthologs
editCORO6 is highly conserved throughout the organisms from vertebrate to fungus, the organisms listed in the table are some representatives.
| Genus and species (Orthologs comparison) | Homo sapiens | Pan troglodytes | Canis familiaris | Anolis carolinensis | Danio rerio | Saccharomyces cerevisiae | Plasmodium falciparum |
|---|---|---|---|---|---|---|---|
| Common name | Human | Chimpanzee | Dog | Lizard | Zebrafish | Baker's Yeast | Malaria parasite |
| Date of divergence from human lineage | 6.3 MYA | 94.2 MYA | 269 MYA | 400.1 MYA | 1215.8 MYA | 1381.2 MYA | |
| Accession number | NP_116243 | XP_001137660 | XP_548302 | XP_0003227217 | NP_956690 | NP_013533 | XP_001350896 |
| Sequence length | 472 aa | 471 aa | 472 aa | 471 aa | 436 aa | 651 aa | 602 aa |
| Sequence identity to human | 96% | 98% | 83% | 78% | 42% | 31% | |
| Sequence similarity to human | 97% | 99% | 90% | 90% | 62% | 52% |
Clinical significance
editThere are several clinical studies about that have been performed by using microarray indicating that CORO6 is positively related to allergic nasal epithelium response to house dust mite allergen in vitro.[12]
References
edit- ^ a b c GRCh38: Ensembl release 89: ENSG00000167549 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000020836 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ de Hostos EL (September 1999). "The coronin family of actin-associated proteins". Trends in Cell Biology. 9 (9): 345–350. doi:10.1016/S0962-8924(99)01620-7. PMID 10461187.
- ^ "Cronin-6 Homo Sapiens". NCBI. Retrieved 28 April 2013.
- ^ "CORO6". GeneCards. Retrieved 28 April 2013.
- ^ "CORO6". HomoloGene. Retrieved 28 April 2013.
- ^ "CORO6 expression level". EST profile. Retrieved 28 April 2013.
- ^ "CORO6". ACEview.
- ^ "WD-40 superfamily". conserved domain. Retrieved 28 April 2013.
- ^ "Allergic nasal epithelium response to house dust mite allergen in vitro.pnj". GEO profile. Retrieved 9 May 2013.
External links
edit- Human CORO6 genome location and CORO6 gene details page in the UCSC Genome Browser.