CPSF3

Cleavage and polyadenylation specificity factor subunit 3 is a protein that in humans is encoded by the CPSF3 gene.

CPSF3
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2I7T, 2I7V
Identifiers
Aliases	CPSF3, CPSF-73, CPSF73, cleavage and polyadenylation specific factor 3
External IDs	OMIM: 606029; MGI: 1859328; HomoloGene: 6499; GeneCards: CPSF3; OMA:CPSF3 - orthologs
Gene location (Human) Chr. Chromosome 2 (human) Band 2p25.1 Start 9,423,651 bp End 9,473,101 bp
Gene location (Mouse) Chr. Chromosome 12 (mouse) Band 12|12 A1.3 Start 21,335,392 bp End 21,365,057 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in ganglionic eminence ventricular zone left testis right testis endothelial cell tendon of biceps brachii sperm monocyte stromal cell of endometrium islet of Langerhans Top expressed in primitive streak medullary collecting duct ureter internal carotid artery condyle fossa vas deferens Epithelium of choroid plexus external carotid artery endothelial cell of lymphatic vessel More reference expression data BioGPS n/a
Gene ontology Molecular function 5'-3' exonuclease activity metal ion binding endoribonuclease activity protein binding RNA binding nuclease activity endonuclease activity hydrolase activity Cellular component mRNA cleavage and polyadenylation specificity factor complex nucleoplasm nucleus Biological process mRNA splicing, via spliceosome termination of RNA polymerase II transcription mRNA 3'-end processing by stem-loop binding and cleavage mRNA processing mRNA export from nucleus mRNA polyadenylation mRNA cleavage mRNA 3'-end processing RNA phosphodiester bond hydrolysis, endonucleolytic sno(s)RNA 3'-end processing snoRNA splicing pre-mRNA cleavage required for polyadenylation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 51692 54451 Ensembl ENSG00000119203 ENSMUSG00000054309 UniProt Q9UKF6 Q9QXK7 RefSeq (mRNA) NM_016207 NM_001321833 NM_001321834 NM_001321835 NM_001321836 NM_018813 NM_001364372 NM_001364373 RefSeq (protein) NP_001308762 NP_001308763 NP_001308764 NP_001308765 NP_057291 NP_061283 NP_001351301 NP_001351302 Location (UCSC) Chr 2: 9.42 – 9.47 Mb Chr 12: 21.34 – 21.37 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

External links

• Human CPSF3 genome location and CPSF3 gene details page in the UCSC Genome Browser.

Further reading

• Murthy KG, Manley JL (1995). "The 160-kD subunit of human cleavage-polyadenylation specificity factor coordinates pre-mRNA 3'-end formation". Genes Dev. 9 (21): 2672–83. doi:10.1101/gad.9.21.2672. PMID 7590244.
• Jenny A, Hauri HP, Keller W (1994). "Characterization of cleavage and polyadenylation specificity factor and cloning of its 100-kilodalton subunit". Mol. Cell. Biol. 14 (12): 8183–90. doi:10.1128/MCB.14.12.8183. PMC 359357. PMID 7969155.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Thuresson AC, Aström J, Aström A, et al. (1994). "Multiple forms of poly(A) polymerases in human cells". Proc. Natl. Acad. Sci. U.S.A. 91 (3): 979–83. Bibcode:1994PNAS...91..979T. doi:10.1073/pnas.91.3.979. PMC 521437. PMID 8302877.
• McCracken S, Fong N, Yankulov K, et al. (1997). "The C-terminal domain of RNA polymerase II couples mRNA processing to transcription". Nature. 385 (6614): 357–61. Bibcode:1997Natur.385..357M. doi:10.1038/385357a0. PMID 9002523. S2CID 4307073.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Takagaki Y, Manley JL (2000). "Complex protein interactions within the human polyadenylation machinery identify a novel component". Mol. Cell. Biol. 20 (5): 1515–25. doi:10.1128/MCB.20.5.1515-1525.2000. PMC 85326. PMID 10669729.
• de Vries H, Rüegsegger U, Hübner W, et al. (2000). "Human pre-mRNA cleavage factor II(m) contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781. PMID 11060040.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Kaufmann I, Martin G, Friedlein A, et al. (2005). "Human Fip1 is a subunit of CPSF that binds to U-rich RNA elements and stimulates poly(A) polymerase". EMBO J. 23 (3): 616–26. doi:10.1038/sj.emboj.7600070. PMC 1271804. PMID 14749727.
• Calzado MA, Sancho R, Muñoz E (2004). "Human immunodeficiency virus type 1 Tat increases the expression of cleavage and polyadenylation specificity factor 73-kilodalton subunit modulating cellular and viral expression". J. Virol. 78 (13): 6846–54. doi:10.1128/JVI.78.13.6846-6854.2004. PMC 421638. PMID 15194760.
• Jin J, Smith FD, Stark C, et al. (2004). "Proteomic, functional, and domain-based analysis of in vivo 14-3-3 binding proteins involved in cytoskeletal regulation and cellular organization". Curr. Biol. 14 (16): 1436–50. Bibcode:2004CBio...14.1436J. doi:10.1016/j.cub.2004.07.051. PMID 15324660.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Mandel CR, Kaneko S, Zhang H, et al. (2007). "Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease". Nature. 444 (7121): 953–6. doi:10.1038/nature05363. PMC 3866582. PMID 17128255.
• de la Vega L, Sánchez-Duffhues G, Fresno M, et al. (2007). "The 73 kDa subunit of the CPSF complex binds to the HIV-1 LTR promoter and functions as a negative regulatory factor that is inhibited by the HIV-1 Tat protein". J. Mol. Biol. 372 (2): 317–30. doi:10.1016/j.jmb.2007.06.075. PMID 17669424.