CSPD ([3-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate) is a chemical substance with formula C18H22ClO7P. It is a component of enhanced chemiluminescence enzyme-linked immunosorbent assay (ELISA) kits, used for the detection of minute amounts of various substances such as proteins.[1]

CSPD
Names
IUPAC name
3-(1-Chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate
Identifiers
3D model (JSmol)
ChemSpider
  • InChI=1S/C18H22ClO7P/c1-23-18(12-3-2-4-15(7-12)24-27(20,21)22)17(25-26-18)13-5-11-6-14(17)10-16(19,8-11)9-13/h2-4,7,11,13-14H,5-6,8-10H2,1H3,(H2,20,21,22) checkY
    Key: QWXOJIDBSHLIFI-UHFFFAOYSA-N checkY
  • COC1(C2(C3CC4CC2CC(C4)(C3)Cl)OO1)c5cccc(c5)OP(=O)(O)O
Properties
C18H22ClO7P
Molar mass 416.79 g·mol−1
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Properties

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The molecule CSPD has the following functional groups in the structure: phosphate group, phenyl group, spiro group, methyl ether group, and chlorine group. The ones worth noting are the ones above. None of these groups carry a charge. If there was a charge this would have had a change in the compound's pH, 3D structure, mass and bond angles.[2]

The toxin CSPD effect persister cell formation using MqsR (MqsR, a crucial regulator for quorum sensing and biofilm formation, is a GCU-specific mRNA interferase in Escherichia coli[3]) and persister cells are cells that avoid stress and are characterized by reduced metabolism and other factors.[4][clarification needed]

References

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  1. ^ Kricka, Larry J (1993). "Ultrasensitive immunoassay techniques". Clinical Biochemistry. 26 (5): 325–331. doi:10.1016/0009-9120(93)90108-I. PMID 8299202.
  2. ^ PubChem. "[3-(1-Chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate". pubchem.ncbi.nlm.nih.gov. Retrieved 2021-11-18.
  3. ^ "mqsR - mRNA interferase toxin MqsR - Escherichia coli (strain K12) - mqsR gene & protein". www.uniprot.org. Retrieved 2021-11-19.
  4. ^ Kim, Younghoon; Wood, Thomas K. (2010-01-01). "Toxins Hha and CspD and small RNA regulator Hfq are involved in persister cell formation through MqsR in Escherichia coli". Biochemical and Biophysical Research Communications. 391 (1): 209–213. doi:10.1016/j.bbrc.2009.11.033. ISSN 0006-291X. PMC 2812665. PMID 19909729.