Capicua (protein)

Capicua transcriptional repressor is a protein that in humans is encoded by the CIC gene. Capicua functions as a transcriptional repressor in a way that ensures its impact on the progression of cancer, and plays a significant role in the operation of the central nervous system through its interaction with ataxin 1. The name of the protein derives from the Catalan expression cap-i-cua which literally translates to "head-and-tail".

CIC
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2M41, 4J2J, 4J2L
Identifiers
Aliases	CIC, capicua transcriptional repressor, MRD45, CIC gene
External IDs	OMIM: 612082; MGI: 1918972; HomoloGene: 87637; GeneCards: CIC; OMA:CIC - orthologs
Gene location (Human) Chr. Chromosome 19 (human) Band 19q13.2 Start 42,268,537 bp End 42,295,797 bp
Gene location (Mouse) Chr. Chromosome 7 (mouse) Band 7|7 A3 Start 24,967,129 bp End 24,993,584 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right hemisphere of cerebellum olfactory bulb sural nerve beta cell right testis gastric mucosa left testis right ovary body of uterus anterior pituitary Top expressed in cerebellar cortex neural layer of retina bone marrow olfactory bulb dentate gyrus of hippocampal formation granule cell ventricular zone superior frontal gyrus striatum of neuraxis thymus piriform cortex More reference expression data BioGPS More reference expression data
Gene ontology Molecular function DNA binding protein binding chromatin binding DNA-binding transcription factor activity, RNA polymerase II-specific Cellular component nucleus nucleoplasm intracellular membrane-bounded organelle protein-containing complex Biological process transcription, DNA-templated regulation of transcription, DNA-templated negative regulation of transcription by RNA polymerase II brain development learning memory social behavior negative regulation of transcription, DNA-templated lung alveolus development Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 23152 71722 Ensembl ENSG00000079432 ENSMUSG00000005442 UniProt Q96RK0 Q924A2 RefSeq (mRNA) NM_001304815 NM_015125 NM_001379480 NM_001379482 NM_001379484 NM_001379485 NM_001386298 NM_001110131 NM_001110132 NM_027882 NM_001302811 RefSeq (protein) NP_001291744 NP_055940 NP_001366409 NP_001366411 NP_001366413 NP_001366414 NP_001289740 NP_082158 Location (UCSC) Chr 19: 42.27 – 42.3 Mb Chr 7: 24.97 – 24.99 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Structure

Capicua is a highly conserved protein, with a lot of similarity between human and Drosophila melanogaster homologs. In the human body, capicua exists in two isoforms, the short (CIC-S) and the long (CIC-L) one, which differ in their N-terminal section. The two evolutionarily conserved domains of the protein are HMG-box (high-mobility group box) and the C1 domain: they work together to recognize specific octameric DNA sequences. Capicua also contains a nuclear localisation sequence that allows it to enter the nucleus of the cell.

Schematic illustration of human CIC-S and CIC-L forms of the capicua protein. CIC-L has a unique long N-terminal region compared with CIC-S. The amino acid regions of CIC responsible for the interaction with ATXN1/ATXN1L, 14-3-3, and ERK, the HMG box, nuclear localization signal (NLS), c-Src-mediated phosphorylation site, and C1 domain, are depicted. Numbers indicate amino acid positions. EBS: ERK binding site. From a review by Yoontae Lee, 2020.

Clinical significance

A new disorder called autosomal dominant intellectual developmental disorder 45 caused by mutations of the CIC gene was first described in 2017.

Spinocerebellar ataxia

Capicua forms a complex with ataxin 1 (CIC-ATXN1 complex) and owing to this interaction it plays a crucial role in the development of spinocerebellar ataxia type 1. While in a healthy organism this complex serves to ensure correct cellular function, in patients with ATXN1 mutations a modified complex has a toxic effect on cerebellar cells, resulting in the motor symptoms typical for this disorder. Blocking the formation of the complex in a murine model of ataxia reduces the symptoms.

Tumors

CIC has been shown to act as a tumor suppressor in numerous types of cancer, and, vice versa, mutations of CIC have been found in some types of tumors. According to a review published in 2020, CIC mutations were most often discovered in oligodendroglioma. A genomic translocation resulting in the formation of a hybrid CIC-DUX4 gene may cause an aggressive Ewing-like sarcoma. Instead of acting as a suppressor, a hybrid protein produced by fusion of CIC and DUX4 has been shown to act as an activator of genes.

According to a review published in 2017, the CIC gene is deleted in 46-53% of analyzed oligodendroglioma tumors as part of the 1p/19q codeletion, a mutation that may also affect the FUBP1 gene.

Primary sarcoma with a chimeric CIC-DUX4 protein in a 9 year-old girl, H&E stain, from Nakai et al., 2019.

Ongoing research

According to a study published in 2021, CIC mutations may be among the causes of cerebral folate deficiency.

History

The CIC gene was first identified in Drosophila in 2000. It was shown to encode a transcriptional repressor participating in the regulation of embryogenesis. In a mutated fly which at the embryonic stage had only the first and the last segments present, with the intermediate segments missing, scientists discovered a mutation of the CIC gene, and this prompted them to call the protein capicua ("head-and-tail" in Catalan).

Disrupted embryogenesis in a Drosophila fly due to the cic⁴ mutation affecting the CIC gene. The formation of the fly's intermediate body segments is suppressed, with only the first and the last segments being developed. From Marta Fores et al., 2017.

Interactions

• FOLR1 — capicua has been shown to affect the expression of folate receptor alpha, and CIC mutations might result in cerebral folate deficiency.
• ATXN1 — capicua and ataxin 1 form a complex (ATXN1-CIC) which is crucial for proper brain development.
• DUX4 – chimeric CIC-DUX4 proteins are found in tumors.
• FOXO4 – chimeric CIC-FOXO4 proteins are found in tumors.
• NUTM1 – chimeric CIC-NUTM1 proteins are found in tumors.
• LEUTX – chimeric CIC-LEUTX proteins are found in tumors.

Further reading

• Olsen JV, Blagoev B, Gnad F, et al. (2006). "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks". Cell. 127 (3): 635–48. doi:10.1016/j.cell.2006.09.026. PMID 17081983. S2CID 7827573.
• Lim J, Hao T, Shaw C, et al. (2006). "A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration". Cell. 125 (4): 801–14. doi:10.1016/j.cell.2006.03.032. PMID 16713569. S2CID 13709685.
• Ballif BA, Villén J, Beausoleil SA, et al. (2005). "Phosphoproteomic analysis of the developing mouse brain". Mol. Cell. Proteomics. 3 (11): 1093–101. doi:10.1074/mcp.M400085-MCP200. PMID 15345747.
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. Bibcode:2004PNAS..10112130B. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935.
• Grimwood J, Gordon LA, Olsen A, et al. (2004). "The DNA sequence and biology of human chromosome 19". Nature. 428 (6982): 529–35. Bibcode:2004Natur.428..529G. doi:10.1038/nature02399. PMID 15057824.
• Nagase T, Ishikawa K, Nakajima D, et al. (1997). "Prediction of the coding sequences of unidentified human genes. VII. The complete sequences of 100 new cDNA clones from brain which can code for large proteins in vitro". DNA Res. 4 (2): 141–50. doi:10.1093/dnares/4.2.141. PMID 9205841.

External links

• Human CIC genome location and CIC gene details page in the UCSC Genome Browser.