Centromere protein E

Centromere-associated protein E is a protein that in humans is encoded by the CENPE gene.

CENPE
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1T5C
Identifiers
Aliases	CENPE, CENP-E, KIF10, PPP1R61, MCPH13, Centromere protein E
External IDs	OMIM: 117143; MGI: 1098230; HomoloGene: 20429; GeneCards: CENPE; OMA:CENPE - orthologs
Gene location (Human) Chr. Chromosome 4 (human) Band 4q24 Start 103,105,349 bp End 103,198,445 bp
Gene location (Mouse) Chr. Chromosome 3 (mouse) Band 3 G3|3 62.55 cM Start 134,918,298 bp End 134,979,372 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in oocyte ventricular zone testicle secondary oocyte ganglionic eminence gonad trabecular bone bone marrow stromal cell of endometrium bone marrow cells Top expressed in zygote secondary oocyte genital tubercle tail of embryo maxillary prominence ventricular zone primary oocyte mandibular prominence abdominal wall vas deferens More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding ATPase activity ATP binding protein binding microtubule motor activity microtubule binding kinetochore binding Cellular component spindle kinesin complex chromosome nucleus outer kinetochore chromosome, centromeric region membrane midbody microtubule kinetochore condensed chromosome, centromeric region microtubule cytoskeleton mitotic spindle midzone cytoskeleton cytoplasm cytosol kinetochore microtubule nucleoplasm spindle midzone Biological process regulation of mitotic metaphase/anaphase transition cell cycle positive regulation of protein kinase activity multicellular organism development cell division antigen processing and presentation of exogenous peptide antigen via MHC class II kinetochore assembly microtubule-based movement sister chromatid cohesion retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum metaphase plate congression lateral attachment of mitotic spindle microtubules to kinetochore mitotic cell cycle mitotic spindle organization mitotic chromosome movement towards spindle pole microtubule plus-end directed mitotic chromosome migration chromosome segregation mitotic metaphase plate congression attachment of mitotic spindle microtubules to kinetochore Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1062 229841 Ensembl ENSG00000138778 ENSMUSG00000045328 UniProt Q02224 Q6RT24 RefSeq (mRNA) NM_001286734 NM_001813 NM_173762 RefSeq (protein) NP_001273663 NP_001804 NP_776123 Location (UCSC) Chr 4: 103.11 – 103.2 Mb Chr 3: 134.92 – 134.98 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Centromere-associated protein E is a kinesin-like motor protein that accumulates in the G2 phase of the cell cycle. Unlike other centromere-associated proteins, it is not present during interphase and first appears at the centromere region of chromosomes during prometaphase. CENPE is proposed to be one of the motors responsible for mammalian chromosome movement and/or spindle elongation.

CENPE is also called Kinesin-7.

Clinical significance

Mutations in CENPE result in autosomal recessive primary microcephaly type 13, which includes skeletal abnormalities and immunodeficiency.

See also

• CENPF
• CENPJ
• CENPT

Further reading

• Yen TJ, Li G, Schaar BT, Szilak I, Cleveland DW (1992). "CENP-E is a putative kinetochore motor that accumulates just before mitosis". Nature. 359 (6395): 536–9. Bibcode:1992Natur.359..536Y. doi:10.1038/359536a0. PMID 1406971. S2CID 4306312.
• Yen TJ, Compton DA, Wise D, Zinkowski RP, Brinkley BR, Earnshaw WC, et al. (1991). "CENP-E, a novel human centromere-associated protein required for progression from metaphase to anaphase". EMBO J. 10 (5): 1245–54. doi:10.1002/j.1460-2075.1991.tb08066.x. PMC 452779. PMID 2022189.
• Thrower DA, Jordan MA, Schaar BT, Yen TJ, Wilson L (1995). "Mitotic HeLa cells contain a CENP-E-associated minus end-directed microtubule motor". EMBO J. 14 (5): 918–26. doi:10.1002/j.1460-2075.1995.tb07073.x. PMC 398164. PMID 7889940.
• Wood KW, Sakowicz R, Goldstein LS, Cleveland DW (1997). "CENP-E is a plus end-directed kinetochore motor required for metaphase chromosome alignment". Cell. 91 (3): 357–66. doi:10.1016/S0092-8674(00)80419-5. PMID 9363944. S2CID 12216144.
• Cooke CA, Schaar B, Yen TJ, Earnshaw WC (1998). "Localization of CENP-E in the fibrous corona and outer plate of mammalian kinetochores from prometaphase through anaphase". Chromosoma. 106 (7): 446–55. doi:10.1007/s004120050266. PMID 9391217. S2CID 18884489.
• Zecevic M, Catling AD, Eblen ST, Renzi L, Hittle JC, Yen TJ, et al. (1998). "Active MAP kinase in mitosis: localization at kinetochores and association with the motor protein CENP-E". J. Cell Biol. 142 (6): 1547–58. doi:10.1083/jcb.142.6.1547. PMC 2141767. PMID 9744883.
• Chan GK, Schaar BT, Yen TJ (1998). "Characterization of the kinetochore binding domain of CENP-E reveals interactions with the kinetochore proteins CENP-F and hBUBR1". J. Cell Biol. 143 (1): 49–63. doi:10.1083/jcb.143.1.49. PMC 2132809. PMID 9763420.
• Chan GK, Jablonski SA, Sudakin V, Hittle JC, Yen TJ (1999). "Human BUBR1 is a mitotic checkpoint kinase that monitors CENP-E functions at kinetochores and binds the cyclosome/APC". J. Cell Biol. 146 (5): 941–54. doi:10.1083/jcb.146.5.941. PMC 2169490. PMID 10477750.
• Ashar HR, James L, Gray K, Carr D, Black S, Armstrong L, et al. (2000). "Farnesyl transferase inhibitors block the farnesylation of CENP-E and CENP-F and alter the association of CENP-E with the microtubules". J. Biol. Chem. 275 (39): 30451–7. doi:10.1074/jbc.M003469200. PMID 10852915.
• Uren AG, Wong L, Pakusch M, Fowler KJ, Burrows FJ, Vaux DL, et al. (2001). "Survivin and the inner centromere protein INCENP show similar cell-cycle localization and gene knockout phenotype". Curr. Biol. 10 (21): 1319–28. Bibcode:2000CBio...10.1319U. doi:10.1016/S0960-9822(00)00769-7. PMID 11084331. S2CID 18455745.
• Suzuki Y, Tsunoda T, Sese J, Taira H, Mizushima-Sugano J, Hata H, et al. (2001). "Identification and characterization of the potential promoter regions of 1031 kinds of human genes". Genome Res. 11 (5): 677–84. doi:10.1101/gr.gr-1640r. PMC 311086. PMID 11337467.
• Liu ST, Chan GK, Hittle JC, Fujii G, Lees E, Yen TJ (2003). "Human MPS1 kinase is required for mitotic arrest induced by the loss of CENP-E from kinetochores". Mol. Biol. Cell. 14 (4): 1638–51. doi:10.1091/mbc.02-05-0074. PMC 153128. PMID 12686615.
• Brandenberger R, Wei H, Zhang S, Lei S, Murage J, Fisk GJ, et al. (2005). "Transcriptome characterization elucidates signaling networks that control human ES cell growth and differentiation". Nat. Biotechnol. 22 (6): 707–16. doi:10.1038/nbt971. PMID 15146197. S2CID 27764390.
• Garcia-Saez I, Blot D, Kahn R, Kozielski F (2005). "Crystallization and preliminary crystallographic analysis of the motor domain of human kinetochore-associated protein CENP-E using an automated crystallization procedure". Acta Crystallogr. D. 60 (Pt 6): 1158–60. doi:10.1107/S0907444904009564. PMID 15159587.
• Tanudji M, Shoemaker J, L'Italien L, Russell L, Chin G, Schebye XM (2005). "Gene silencing of CENP-E by small interfering RNA in HeLa cells leads to missegregation of chromosomes after a mitotic delay". Mol. Biol. Cell. 15 (8): 3771–81. doi:10.1091/mbc.E03-07-0482. PMC 491836. PMID 15181147.
• Garcia-Saez I, Yen T, Wade RH, Kozielski F (2004). "Crystal structure of the motor domain of the human kinetochore protein CENP-E". J. Mol. Biol. 340 (5): 1107–16. doi:10.1016/j.jmb.2004.05.053. PMID 15236970.
• Kurasawa Y, Earnshaw WC, Mochizuki Y, Dohmae N, Todokoro K (2005). "Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation". EMBO J. 23 (16): 3237–48. doi:10.1038/sj.emboj.7600347. PMC 514520. PMID 15297875.
• Mao Y, Desai A, Cleveland DW (2005). "Microtubule capture by CENP-E silences BubR1-dependent mitotic checkpoint signaling". J. Cell Biol. 170 (6): 873–80. doi:10.1083/jcb.200505040. PMC 2171436. PMID 16144904.
• Nousiainen M, Silljé HH, Sauer G, Nigg EA, Körner R (2006). "Phosphoproteome analysis of the human mitotic spindle". Proc. Natl. Acad. Sci. U.S.A. 103 (14): 5391–6. Bibcode:2006PNAS..103.5391N. doi:10.1073/pnas.0507066103. PMC 1459365. PMID 16565220.