Class 16 may refer to:
- Belgian Railways Class 16
- British Rail Class 16
- DRG Class 16, a class of German six-coupled, express train, steam locomotives operated by the Deutsche Reichsbahn which included the:
- Class 16.0: the Oldenburg S 10
- Class 16.0II: the BBÖ 310, PKP Class Pn12 and PKP Class Pn11
- DRG Class E 16
- LSWR G16 class
- LSWR H16 class
- Pennsylvania Railroad class D16
- Rhodesia Railways 16A class
- South African Class NG G16 2-6-2+2-6-2
- VR Class Dr16