DDOST

Dolichyl-diphosphooligosaccharide—protein glycosyltransferase 48 kDa subunit is an enzyme that in humans is encoded by the DDOST gene.

DDOST
Identifiers
Aliases	DDOST, AGER1, CDG1R, OKSWcl45, OST, OST48, WBP1, dolichyl-diphosphooligosaccharide--protein glycosyltransferase non-catalytic subunit, GATD6
External IDs	OMIM: 602202; MGI: 1194508; HomoloGene: 3821; GeneCards: DDOST; OMA:DDOST - orthologs
Gene location (Human) Chr. Chromosome 1 (human) Band 1p36.12 Start 20,651,767 bp End 20,661,544 bp
Gene location (Mouse) Chr. Chromosome 4 (mouse) Band 4|4 D3 Start 138,032,041 bp End 138,039,939 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in corpus epididymis stromal cell of endometrium body of pancreas caput epididymis islet of Langerhans rectum smooth muscle tissue placenta beta cell spleen Top expressed in parotid gland lacrimal gland seminal vesicula gastrula dermis islet of Langerhans molar stroma of bone marrow vestibular sensory epithelium crypt of lieberkuhn of small intestine More reference expression data BioGPS More reference expression data
Gene ontology Molecular function dolichyl-diphosphooligosaccharide-protein glycotransferase activity protein binding oligosaccharyl transferase activity Cellular component integral component of membrane endoplasmic reticulum membrane membrane intracellular membrane-bounded organelle endoplasmic reticulum oligosaccharyltransferase complex plasma membrane azurophil granule membrane protein-containing complex Biological process response to cytokine protein N-linked glycosylation via asparagine protein glycosylation protein N-linked glycosylation T cell activation neutrophil degranulation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1650 13200 Ensembl ENSG00000244038 ENSMUSG00000028757 UniProt P39656 O54734 RefSeq (mRNA) NM_005216 NM_007838 RefSeq (protein) NP_005207 NP_031864 Location (UCSC) Chr 1: 20.65 – 20.66 Mb Chr 4: 138.03 – 138.04 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

This gene encodes a component of the oligosaccharyltransferase complex which catalyzes the transfer of high-mannose oligosaccharides to asparagine residues on nascent polypeptides in the lumen of the rough endoplasmic reticulum. The protein complex co-purifies with ribosomes. The product of this gene is also implicated in the processing of advanced glycation endproducts (AGEs), which form from non-enzymatic reactions between sugars and proteins or lipids and are associated with aging and hyperglycemia.

Further reading

• Kumar V, Korza G, Heinemann FS, Ozols J (1995). "Human oligosaccharyltransferase: isolation, characterization, and the complete amino acid sequence of 50-kDa subunit". Arch. Biochem. Biophys. 320 (2): 217–23. doi:10.1016/0003-9861(95)90003-9. PMID 7625827.
• Nagase T, Miyajima N, Tanaka A, et al. (1995). "Prediction of the coding sequences of unidentified human genes. III. The coding sequences of 40 new genes (KIAA0081-KIAA0120) deduced by analysis of cDNA clones from human cell line KG-1". DNA Res. 2 (1): 37–43. doi:10.1093/dnares/2.1.37. PMID 7788527.
• Stitt AW, He C, Vlassara H (1999). "Characterization of the advanced glycation end-product receptor complex in human vascular endothelial cells". Biochem. Biophys. Res. Commun. 256 (3): 549–56. doi:10.1006/bbrc.1999.0291. PMID 10080935.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Lu C, He JC, Cai W, et al. (2004). "Advanced glycation endproduct (AGE) receptor 1 is a negative regulator of the inflammatory response to AGE in mesangial cells". Proc. Natl. Acad. Sci. U.S.A. 101 (32): 11767–72. Bibcode:2004PNAS..10111767L. doi:10.1073/pnas.0401588101. PMC 511050. PMID 15289604.
• Suzuki Y, Yamashita R, Shirota M, et al. (2004). "Sequence Comparison of Human and Mouse Genes Reveals a Homologous Block Structure in the Promoter Regions". Genome Res. 14 (9): 1711–8. doi:10.1101/gr.2435604. PMC 515316. PMID 15342556.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Shibatani T, David LL, McCormack AL, et al. (2005). "Proteomic analysis of mammalian oligosaccharyltransferase reveals multiple subcomplexes that contain Sec61, TRAP, and two potential new subunits". Biochemistry. 44 (16): 5982–92. doi:10.1021/bi047328f. PMID 15835887.
• Ewing RM, Chu P, Elisma F, et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931.