DNA polymerase nu

Polymerase (DNA directed) nu is a protein in humans that is encoded by the POLN gene. It is a family A DNA polymerase, considered to be the least effective of the polymerase enzymes. However, DNA polymerase nu plays an active role in homology repair during cellular responses to crosslinks, fulfilling its role in a complex with helicase.

POLN
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4XVI, 4XVK, 4XVL, 4XVM
Identifiers
Aliases	POLN, POL4P, polymerase (DNA) nu, DNA polymerase nu
External IDs	OMIM: 610887; MGI: 2675617; HomoloGene: 129696; GeneCards: POLN; OMA:POLN - orthologs
Gene location (Human) Chr. Chromosome 4 (human) Band 4p16.3 Start 2,071,918 bp End 2,242,121 bp
Gene location (Mouse) Chr. Chromosome 5 (mouse) Band 5|5 B2 Start 34,164,523 bp End 34,326,792 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in caudate nucleus putamen nucleus accumbens right frontal lobe Brodmann area 9 testicle anterior cingulate cortex left testis right testis cerebellar hemisphere Top expressed in spermatocyte spermatid brown adipose tissue seminiferous tubule lens lumbar subsegment of spinal cord white adipose tissue primary oocyte zygote epithelium of lens More reference expression data BioGPS n/a
Gene ontology Molecular function transferase activity DNA binding nucleotidyltransferase activity 5'-3' exonuclease activity protein binding cyclin binding DNA-directed DNA polymerase activity nucleic acid binding Cellular component cytoplasm nucleolus nucleoplasm nucleus Biological process cellular response to DNA damage stimulus DNA replication double-strand break repair via homologous recombination DNA repair DNA-dependent DNA replication interstrand cross-link repair translesion synthesis nucleic acid phosphodiester bond hydrolysis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 353497 272158 Ensembl ENSG00000130997 ENSMUSG00000045102 UniProt Q7Z5Q5 Q7TQ07 RefSeq (mRNA) NM_181808 NM_001289803 NM_001289804 NM_181857 RefSeq (protein) NP_861524 NP_001276732 NP_001276733 NP_862905 Location (UCSC) Chr 4: 2.07 – 2.24 Mb Chr 5: 34.16 – 34.33 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Further reading

• Zietlow L, Smith LA, Bessho M, Bessho T (December 2009). "Evidence for the involvement of human DNA polymerase N in the repair of DNA interstrand cross-links". Biochemistry. 48 (49): 11817–24. doi:10.1021/bi9015346. PMC 2790558. PMID 19908865.
• Bi X, Barkley LR, Slater DM, Tateishi S, Yamaizumi M, Ohmori H, Vaziri C (May 2006). "Rad18 regulates DNA polymerase kappa and is required for recovery from S-phase checkpoint-mediated arrest". Molecular and Cellular Biology. 26 (9): 3527–40. doi:10.1128/MCB.26.9.3527-3540.2006. PMC 1447421. PMID 16611994.
• Moldovan GL, Madhavan MV, Mirchandani KD, McCaffrey RM, Vinciguerra P, D'Andrea AD (February 2010). "DNA polymerase POLN participates in cross-link repair and homologous recombination". Molecular and Cellular Biology. 30 (4): 1088–96. doi:10.1128/MCB.01124-09. PMC 2815579. PMID 19995904.
• Marini F, Kim N, Schuffert A, Wood RD (August 2003). "POLN, a nuclear PolA family DNA polymerase homologous to the DNA cross-link sensitivity protein Mus308". The Journal of Biological Chemistry. 278 (34): 32014–9. doi:10.1074/jbc.M305646200. PMID 12794064.
• Arana ME, Takata K, Garcia-Diaz M, Wood RD, Kunkel TA (February 2007). "A unique error signature for human DNA polymerase nu". DNA Repair. 6 (2): 213–23. doi:10.1016/j.dnarep.2006.09.012. PMC 1950682. PMID 17118716.
• Göhler T, Sabbioneda S, Green CM, Lehmann AR (January 2011). "ATR-mediated phosphorylation of DNA polymerase η is needed for efficient recovery from UV damage". The Journal of Cell Biology. 192 (2): 219–27. doi:10.1083/jcb.201008076. PMC 3172178. PMID 21242293.
• Yamanaka K, Minko IG, Takata K, Kolbanovskiy A, Kozekov ID, Wood RD, Rizzo CJ, Lloyd RS (March 2010). "Novel enzymatic function of DNA polymerase nu in translesion DNA synthesis past major groove DNA-peptide and DNA-DNA cross-links". Chemical Research in Toxicology. 23 (3): 689–95. doi:10.1021/tx900449u. PMC 2838406. PMID 20102227.
• Arana ME, Potapova O, Kunkel TA, Joyce CM (November 2011). "Kinetic analysis of the unique error signature of human DNA polymerase ν". Biochemistry. 50 (46): 10126–35. doi:10.1021/bi201197p. PMC 3235957. PMID 22008035.

External links

• PDBe-KB provides an overview of all the structure information available in the PDB for Human DNA polymerase nu