Desosaminyl transferase EryCIII (EC 2.4.1.278, EryCIII) is an enzyme with systematic name dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose:3-alpha-mycarosylerythronolide B 3-dimethylamino-4,6-dideoxy-alpha-D-glucosyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction
| Desosaminyl transferase EryCIII | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.4.1.278 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose + 3-alpha-mycarosylerythronolide B dTDP + erythromycin D
The enzyme is involved in erythromycin biosynthesis.
References
edit- ^ Yuan Y, Chung HS, Leimkuhler C, Walsh CT, Kahne D, Walker S (October 2005). "In vitro reconstitution of EryCIII activity for the preparation of unnatural macrolides". Journal of the American Chemical Society. 127 (41): 14128–9. doi:10.1021/ja053704n. PMC 2525786. PMID 16218575.
- ^ Lee HY, Chung HS, Hang C, Khosla C, Walsh CT, Kahne D, Walker S (August 2004). "Reconstitution and characterization of a new desosaminyl transferase, EryCIII, from the erythromycin biosynthetic pathway". Journal of the American Chemical Society. 126 (32): 9924–5. doi:10.1021/ja048836f. PMID 15303858.
- ^ Moncrieffe MC, Fernandez MJ, Spiteller D, Matsumura H, Gay NJ, Luisi BF, Leadlay PF (January 2012). "Structure of the glycosyltransferase EryCIII in complex with its activating P450 homologue EryCII". Journal of Molecular Biology. 415 (1): 92–101. doi:10.1016/j.jmb.2011.10.036. PMC 3391682. PMID 22056329.
External links
edit- Desosaminyl+transferase+EryCIII at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
