EIEIO may refer to:
- The refrain to the children's song, "Old MacDonald Had a Farm"
- Enforce In-Order Execution of I/O, a machine instruction used on the PowerPC computer processor
- Computer bought the farm, an error message with symbol EIEIO, from the GNU Hurd operating system
- EIEIO, the Enhanced Implementation of Emacs Interpreted Objects, an object-oriented elisp system based on the Common Lisp Object System
- "E-I-E-I-O", an episode of Barney & Friends from the fourth season of the show
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