ENPP3

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 is an enzyme that in humans is encoded by the ENPP3 gene.

ENPP3
Identifiers
Aliases	ENPP3, B10, CD203c, NPP3, PD-IBETA, PDNP3, ectonucleotide pyrophosphatase/phosphodiesterase 3
External IDs	OMIM: 602182; MGI: 2143702; HomoloGene: 3683; GeneCards: ENPP3; OMA:ENPP3 - orthologs
Gene location (Human) Chr. Chromosome 6 (human) Band 6q23.2 Start 131,628,442 bp End 131,747,418 bp
Gene location (Mouse) Chr. Chromosome 10 (mouse) Band 10|10 A4 Start 24,648,304 bp End 24,718,721 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in jejunal mucosa mucosa of ileum seminal vesicula duodenum mucosa of paranasal sinus bronchial epithelial cell endometrium buccal mucosa cell tibialis anterior muscle olfactory zone of nasal mucosa Top expressed in epithelium of small intestine conjunctival fornix intestinal villus duodenum parotid gland lacrimal gland cornea jejunum ciliary body left lobe of liver More reference expression data BioGPS n/a
Gene ontology Molecular function nucleoside-triphosphate diphosphatase activity nucleotide diphosphatase activity phosphodiesterase I activity polysaccharide binding metal ion binding scavenger receptor activity NADH pyrophosphatase activity catalytic activity nucleic acid binding hydrolase activity calcium ion binding zinc ion binding Cellular component integral component of membrane membrane integral component of plasma membrane extracellular region perinuclear region of cytoplasm extracellular exosome plasma membrane external side of plasma membrane apical plasma membrane Biological process nucleoside triphosphate catabolic process receptor-mediated endocytosis immune response phosphate-containing compound metabolic process metabolism inorganic diphosphate transport nucleic acid phosphodiester bond hydrolysis basophil activation involved in immune response pyrimidine nucleotide metabolic process negative regulation of mast cell activation involved in immune response ATP metabolic process negative regulation of inflammatory response negative regulation of mast cell proliferation vesicle-mediated transport endocytosis Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 5169 209558 Ensembl ENSG00000154269 ENSMUSG00000019989 UniProt O14638 Q6DYE8 RefSeq (mRNA) NM_005021 NM_134005 NM_145372 RefSeq (protein) NP_005012 NP_598766 Location (UCSC) Chr 6: 131.63 – 131.75 Mb Chr 10: 24.65 – 24.72 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Function

The protein encoded by this gene belongs to a series of ectoenzymes that are involved in hydrolysis of extracellular nucleotides. These ectoenzymes possess ATPase and ATP pyrophosphatase activities and are type II transmembrane proteins. Expression of the related rat mRNA has been found in a subset of immature glial cells and in the alimentary tract. The corresponding rat protein has been detected in the pancreas, small intestine, colon, and liver. The human mRNA is expressed in glioma cells, prostate, and uterus. Expression of the human protein has been detected in uterus, basophils, and mast cells.

This protein has also been used in conjunction with CD63 as a marker for activated basophils in the Basophil Activation Test for IgE mediated allergic reactions.

Further reading

• Bühring HJ, Streble A, Valent P (April 2004). "The basophil-specific ectoenzyme E-NPP3 (CD203c) as a marker for cell activation and allergy diagnosis". International Archives of Allergy and Immunology. 133 (4): 317–29. doi:10.1159/000077351. PMID 15031605. S2CID 12238348.
• Andoh K, Piao JH, Terashima K, Nakamura H, Sano K (September 1999). "Genomic structure and promoter analysis of the ecto-phosphodiesterase I gene (PDNP3) expressed in glial cells". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1446 (3): 213–24. doi:10.1016/s0167-4781(99)00090-1. PMID 10524196.
• Bühring HJ, Seiffert M, Giesert C, Marxer A, Kanz L, Valent P, Sano K (May 2001). "The basophil activation marker defined by antibody 97A6 is identical to the ectonucleotide pyrophosphatase/phosphodiesterase 3". Blood. 97 (10): 3303–5. doi:10.1182/blood.V97.10.3303. PMID 11342463.
• Yano Y, Hayashi Y, Sano K, Shinmaru H, Kuroda Y, Yokozaki H, Yoon S, Kasuga M (November 2003). "Expression and localization of ecto-nucleotide pyrophosphatase/phosphodiesterase I-3 (E-NPP3/CD203c/PD-I beta/B10/gp130RB13-6) in human colon carcinoma". International Journal of Molecular Medicine. 12 (5): 763–6. doi:10.3892/ijmm.12.5.763. PMID 14533006.
• Yano Y, Hayashi Y, Sano K, Nagano H, Nakaji M, Seo Y, Ninomiya T, Yoon S, Yokozaki H, Kasuga M (April 2004). "Expression and localization of ecto-nucleotide pyrophosphatase/phosphodiesterase I-1 (E-NPP1/PC-1) and -3 (E-NPP3/CD203c/PD-Ibeta/B10/gp130(RB13-6)) in inflammatory and neoplastic bile duct diseases". Cancer Letters. 207 (2): 139–47. doi:10.1016/j.canlet.2003.11.002. PMID 15072822.
• Elbatarny HS, Maurice DH (October 2005). "Leptin-mediated activation of human platelets: involvement of a leptin receptor and phosphodiesterase 3A-containing cellular signaling complex". American Journal of Physiology. Endocrinology and Metabolism. 289 (4): E695-702. doi:10.1152/ajpendo.00125.2005. PMID 15886225.
• Kimura K, Wakamatsu A, Suzuki Y, Ota T, Nishikawa T, Yamashita R, Yamamoto J, Sekine M, Tsuritani K, Wakaguri H, Ishii S, Sugiyama T, Saito K, Isono Y, Irie R, Kushida N, Yoneyama T, Otsuka R, Kanda K, Yokoi T, Kondo H, Wagatsuma M, Murakawa K, Ishida S, Ishibashi T, Takahashi-Fujii A, Tanase T, Nagai K, Kikuchi H, Nakai K, Isogai T, Sugano S (January 2006). "Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes". Genome Research. 16 (1): 55–65. doi:10.1101/gr.4039406. PMC 1356129. PMID 16344560.
• Ocmant A, Peignois Y, Mulier S, Hanssens L, Michils A, Schandené L (March 2007). "Flow cytometry for basophil activation markers: the measurement of CD203c up-regulation is as reliable as CD63 expression in the diagnosis of cat allergy". Journal of Immunological Methods. 320 (1–2): 40–8. doi:10.1016/j.jim.2006.12.002. PMID 17275019.