ENTPD2

Ectonucleoside triphosphate diphosphohydrolase 2 is an enzyme that in humans is encoded by the ENTPD2 gene.

ENTPD2
Identifiers
Aliases	ENTPD2, CD39L1, NTPDase-2, ectonucleoside triphosphate diphosphohydrolase 2
External IDs	OMIM: 602012; MGI: 1096863; HomoloGene: 20333; GeneCards: ENTPD2; OMA:ENTPD2 - orthologs
Gene location (Human) Chr. Chromosome 9 (human) Band 9q34.3 Start 137,048,107 bp End 137,054,061 bp
Gene location (Mouse) Chr. Chromosome 2 (mouse) Band 2|2 A3 Start 25,285,886 bp End 25,291,333 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in spinal ganglia tibial nerve trigeminal ganglion mucosa of transverse colon right uterine tube amygdala ventral tegmental area skin of abdomen hypothalamus sural nerve Top expressed in seminal vesicula sciatic nerve ankle tunica adventitia of aorta lip skin of external ear carotid body white adipose tissue aortic valve extraocular muscle More reference expression data BioGPS More reference expression data
Gene ontology Molecular function nucleotide binding nucleoside-diphosphatase activity hydrolase activity ATP binding nucleoside-triphosphatase activity Cellular component integral component of membrane endoplasmic reticulum membrane membrane endoplasmic reticulum extracellular exosome plasma membrane basement membrane Biological process G protein-coupled receptor signaling pathway purine ribonucleoside diphosphate catabolic process platelet activation nucleobase-containing small molecule catabolic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 954 12496 Ensembl ENSG00000054179 ENSMUSG00000015085 UniProt Q9Y5L3 O55026 RefSeq (mRNA) NM_001246 NM_203468 NM_009849 RefSeq (protein) NP_001237 NP_982293 NP_033979 Location (UCSC) Chr 9: 137.05 – 137.05 Mb Chr 2: 25.29 – 25.29 Mb PubMed search
Wikidata
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The protein encoded by this gene is the type 2 enzyme of the ecto-nucleoside triphosphate diphosphohydrolase family (E-NTPDase). E-NTPDases are a family of ecto-nucleosidases that hydrolyze 5'-triphosphates. This ecto-ATPase is an integral membrane protein. Alternative splicing of this gene results in multiple transcript variants.

It has been shown, by scientists from the University of Warwick, that E-NTPDase2 stimulates the growth of the eye: by testing the enzyme on tadpoles, the tadpoles were found to develop extra eyes on their body.

Embryonic yolk sac development

The mouse embryonic yolk sac, comprising the visceral yolk sac (VYS) and parietal yolk sac (PYS), serves as a materno-fetal exchange system, facilitating the transfer of nutrients and removal of wastes. One study aimed to analyze gene expression in VYS and PYS endodermal cells and identify novel genetic markers from microarray data, with a focus on Apoa4, Lrp2, Fxyd2, Slc34a3, and Entpd2, which showed predominant expression in VYS epithelial cells. The study extracted RNA from VYS, PYS, placenta, liver, and small intestine tissues from C3H/HeSlc strain mice. For ENTPD2 (Entpd2) mRNA analysis, digoxigenin-labeled riboprobes were prepared using a DIG RNA labeling kit. Notably, not every VYS epithelial cell showed expression of Entpd2 and Slc34a3, and Entpd2 was additionally detected in the myometrium. The findings suggest that Apoa4, Lrp2, Fxyd2, Slc34a3, and Entpd2, along with Gkn2 and Pga5, can serve as genetic markers for VYS epithelial cells and PYS cells, respectively.

Neuroinflammation

This study investigated the regulation of the ENTPD2 gene and the production of the NTPDase2 protein in brain cells during neuroinflammatory and neurodegenerative conditions. The research primarily focused on rat primary cortical astrocytes and the OLN93 oligodendroglial cell line. The analysis found that NTPDase2-mRNA was the most abundant among ectonucleotidase transcripts in both cell types. In primary astrocytes, NTPDase2-mRNA significantly exceeded other transcripts. When exposed to inflammatory mediators, including IL-6, IL-1β, TNFα, and IFNγ, for 8 and 24 hours, the expression of Entpd2 (the gene encoding NTPDase2) in primary astrocytes remained unaffected at both mRNA and protein levels. However, ATP and the anti-inflammatory cytokine IL-10 increased the levels of Entpd2 mRNA and protein. These findings provide valuable insights into the regulation of NTPDase2 in neuroinflammatory conditions, specifically highlighting the lack of impact on Entpd2 expression by certain proinflammatory cytokines in primary astrocytes.

Taste cell differentiation

The study focuses on the turnover of taste cells, which are constantly replaced throughout an animal's life. The discovery that the transcription factor Etv1 plays a role in regulating the differentiation of taste cells responsible for sweet, umami, and salty tastes is significant. The study examined the expression of certain genes, including Entpd2, in circumvallate papillae (structures on the tongue containing taste buds) of both wild-type (WT) mice and those with Etv1 deficiency (Etv1C/C mice). There is a potential link between Etv1, a transcription factor discussed in the study, and the regulation or expression of Entpd2 in taste cells. This finding advances our understanding of the molecular mechanisms involved in taste cell homeostasis and provides new insights into the lineage of taste cells.

The SARS-CoV-2 virus, responsible for COVID-19, may directly affect taste receptor cells (TRCs) in the oral cavity. The virus binds to angiotensin-converting enzyme 2 (ACE2) on a subset of TRCs, specifically type II cells in taste buds. Biopsies from COVID-19 patients with taste changes confirmed the presence of replicating virus in these cells. The study uses ENTPD2 (ectonucleoside triphosphate diphosphohydrolase 2) as a marker for type I taste cells. The study's findings suggest that there is no overlap of ACE2 (the receptor for the SARS-CoV-2 virus causing COVID-19) with the probe for the transcript of ENTPD2. This suggests that ACE2 is not expressed in the same cells as ENTPD2 in taste buds, providing information about the distribution of ACE2 in relation to different types of taste cells. This could be crucial in understanding how SARS-CoV-2 interacts with specific cells in the oral cavity and how it might impact taste perception.The disruption of stem cells in taste papillae during infection suggests a potential mechanism for sudden taste changes in COVID-19 patients, indicating the need for further research into the virus's impact on taste bud dynamics during and after infection.

Taste sensitivity

Exploring the function of taste buds, this study examines gene-targeted Entpd2-null mice, globally lacking the NTPDase2 enzyme. The Entpd2-null mice exhibited normal numbers and sizes of taste buds. Luciferin/luciferase assays performed on the circumvallate tissue of these knockout mice revealed heightened levels of extracellular ATP. Electrophysiological recordings from both the chorda tympani and glossopharyngeal taste nerves indicated reduced responses to all taste stimuli in Entpd2-null mice. Notably, the depressions were more pronounced in the glossopharyngeal nerve compared to the chorda tympani nerve, encompassing all taste qualities. Specifically, responses to sweet and umami stimuli were more significantly affected in the chorda tympani. The study proposes that the elevated extracellular ATP levels in Entpd2-knockout mice may desensitize P2X receptors associated with nerve fibers, leading to a dampening of taste responses.

Scientist employed various techniques to investigate how the removal of the Entpd2 gene affects taste epithelia. Initially, they utilized RT-PCR to assess the presence of NTPDase2 mRNA in pooled taste buds from fungiform and circumvallate papillae. The results demonstrated that the genetic deletion of Entpd2 successfully eradicated the expression of NTPDase2 mRNA in taste buds. To confirm the presence of all three types of taste cells in the knockout (KO) mice, RT-PCR was used to examine the expression of specific markers for each cell type. Specifically, they looked for GLAST for type I cells, α-gustducin, transient receptor potential melastatin 5 (TRPM5), and phospholipase C β2 (PLCβ2) for type II cells, and synaptosomal-associated protein 25 (SNAP25) for type III cells. The PCR results indicate the presence of all three cell types in both circumvallate and fungiform taste buds of the KO mice. Furthermore, to confirm that NTPDase2 is the sole functional ectoATPase in taste buds, there was comparison of ectoATPase activity in circumvallate papillae between wild-type (WT) and Entpd2-KO animals. Using two different substances, ADP and ATP, helped distinguish specific staining for ectoATPase, representing NTPDase2 or NTPDase8 (26), from less specific nucleotidases that break down both ADP and ATP. In the case of animals with the usual genetic makeup (WT), there was an observed a concentrated reaction product in taste buds when ATP, not ADP, was the substance used. This highlights the high precision of the ectonucleotidase in taste buds, corresponding to the presence of NTPDase2. Conversely, in animals lacking the Entpd2 gene (Entpd2-KO), there was no detectable ectoATPase activity in taste buds when ATP was used, confirming NTPDase2's significant role in degrading ATP in this system. In both the usual genetic makeup and the Entpd2-KO groups, nerve bundles beneath taste buds showed nucleotidase activity when ADP was the substance used, indicating the existence of a different nucleotidase in and around these nerve bundles.

To assess the impact of removing the Entpd2 gene on taste bud synaptic function, the researchers measured responses to taste stimuli using whole-nerve recordings from the chorda tympani and glossopharyngeal nerves in both normal and Entpd2-null animals. The animals lacking Entpd2 exhibited decreased responses to all taste qualities in both nerves. Findings indicate that the failure to break down ATP and its buildup in the taste tissue of Entpd2-knockout mice leads to reduced responses to all taste qualities.

The central discovery of this study is that the genetic removal of NTPDase2, the sole ectoATPase expressed in taste buds, leads to a decline in neural responses to taste stimuli. Despite the unaffected taste bud numbers and cell types in the knockout, the reduced responsiveness is likely due to the absence of ATP degradation, resulting in elevated tissue levels of ATP. Since ATP activates P2X receptors on gustatory nerve fibers, essential for neurotransmission in the taste system, the genetic deletion of ectoATPase is proposed to disrupt purinergic transmission at this critical synapse. In parallel, the area surrounding inactive taste tissue in Entpd2-null mice exhibits heightened nanomolar ATP concentrations, indicating a connection to the desensitization of P2X3 homomers. This suggests a potential link to the observed decrease in responsiveness. Furthermore, the distinctive presence of P2X2 and P2X3 subunits in different taste nerves may elucidate the specific impact on taste perception.

Further reading

• Javed R, Yarimizu K, Pelletier N, Li C, Knowles AF (June 2007). "Mutagenesis of lysine 62, asparagine 64, and conserved region 1 reduces the activity of human ecto-ATPase (NTPDase 2)". Biochemistry. 46 (22): 6617–6627. doi:10.1021/bi700036e. PMID 17489562.
• Mukasa T, Lee Y, Knowles AF (August 2005). "Either the carboxyl- or the amino-terminal region of the human ecto-ATPase (E-NTPDase 2) confers detergent and temperature sensitivity to the chicken ecto-ATP-diphosphohydrolase (E-NTPDase 8)". Biochemistry. 44 (33): 11160–11170. doi:10.1021/bi050019k. PMID 16101300.
• Jhandier MN, Kruglov EA, Lavoie EG, Sévigny J, Dranoff JA (June 2005). "Portal fibroblasts regulate the proliferation of bile duct epithelia via expression of NTPDase2". The Journal of Biological Chemistry. 280 (24): 22986–22992. doi:10.1074/jbc.M412371200. PMID 15799977.
• Dranoff JA, Kruglov EA, Toure J, Braun N, Zimmermann H, Jain D, Knowles AF, Sévigny J (November 2004). "Ectonucleotidase NTPDase2 is selectively down-regulated in biliary cirrhosis". Journal of Investigative Medicine. 52 (7): 475–482. doi:10.1136/jim-52-07-42. PMID 15651265. S2CID 206995030.
• Alvarado-Castillo C, Harden TK, Boyer JL (January 2005). "Regulation of P2Y1 receptor-mediated signaling by the ectonucleoside triphosphate diphosphohydrolase isozymes NTPDase1 and NTPDase2". Molecular Pharmacology. 67 (1): 114–122. doi:10.1124/mol.104.006908. PMID 15496502. S2CID 27764870.
• Knowles AF, Chiang WC (October 2003). "Enzymatic and transcriptional regulation of human ecto-ATPase/E-NTPDase 2". Archives of Biochemistry and Biophysics. 418 (2): 217–227. doi:10.1016/j.abb.2003.08.007. PMID 14522593.
• Mateo J, Kreda S, Henry CE, Harden TK, Boyer JL (October 2003). "Requirement of Cys399 for processing of the human ecto-ATPase (NTPDase2) and its implications for determination of the activities of splice variants of the enzyme". The Journal of Biological Chemistry. 278 (41): 39960–39968. doi:10.1074/jbc.M307854200. PMID 12888562.
• Grinthal A, Guidotti G (February 2002). "Transmembrane domains confer different substrate specificities and adenosine diphosphate hydrolysis mechanisms on CD39, CD39L1, and chimeras". Biochemistry. 41 (6): 1947–1956. doi:10.1021/bi015563h. PMID 11827541.
• Suzuki H, Fukunishi Y, Kagawa I, Saito R, Oda H, Endo T, Kondo S, Bono H, Okazaki Y, Hayashizaki Y (October 2001). "Protein-protein interaction panel using mouse full-length cDNAs". Genome Research. 11 (10): 1758–1765. doi:10.1101/gr.180101. PMC 311163. PMID 11591653.
• Mateo J, Harden TK, Boyer JL (September 1999). "Functional expression of a cDNA encoding a human ecto-ATPase". British Journal of Pharmacology. 128 (2): 396–402. doi:10.1038/sj.bjp.0702805. PMC 1571647. PMID 10510450.