In molecular biology, the EcoEI R protein C-terminal domain is a protein domain found at the C-terminus of both the R subunit of type I restriction enzymes and the Res subunit of type III restriction enzymes. The type I enzymes include EcoEI, which recognises 5'-GAGN(7)ATGC-3; the R protein (HsdR) is required for both nuclease and ATPase activity.[1][2]
| EcoEI_R_C | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| Symbol | EcoEI_R_C | ||||||||
| Pfam | PF08463 | ||||||||
| InterPro | IPR013670 | ||||||||
| |||||||||
References
edit- ^ Murray NE, Daniel AS, Cowan GM, Sharp PM (July 1993). "Conservation of motifs within the unusually variable polypeptide sequences of type I restriction and modification enzymes". Mol. Microbiol. 9 (1): 133–43. doi:10.1111/j.1365-2958.1993.tb01675.x. PMID 8412658. S2CID 45127462.
- ^ Makovets S, Doronina VA, Murray NE (August 1999). "Regulation of endonuclease activity by proteolysis prevents breakage of unmodified bacterial chromosomes by type I restriction enzymes". Proc. Natl. Acad. Sci. U.S.A. 96 (17): 9757–62. Bibcode:1999PNAS...96.9757M. doi:10.1073/pnas.96.17.9757. PMC 22283. PMID 10449767.