Ent-kaurene oxidase (EC 1.14.14.86, Formerly EC 1.14.13.78) is an enzyme with systematic name ent-kaur-16-ene,NADPH:oxygen oxidoreductase (hydroxylating).[1][2][3] This enzyme catalyses the following chemical reaction
| Ent-kaurene oxidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.14.14.86 | ||||||||
| CAS no. | 149565-67-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- ent-kaur-16-ene + 3 NADPH + 3 H+ + 3 O2 ent-kaur-16-en-19-oate + 3 NADP+ + 4 H2O (overall reaction)
- (1a) ent-kaur-16-ene + NADPH + H+ + O2 ent-kaur-16-en-19-ol + NADP+ + H2O
- (1b) ent-kaur-16-en-19-ol + NADPH + H+ + O2 ent-kaur-16-en-19-al + NADP+ + 2 H2O
- (1c) ent-kaur-16-en-19-al + NADPH + O2 ent-kaur-16-en-19-oate + NADP+ + H2O
Ent-kaurene oxidase requires cytochrome P450 and is inhibited by paclobutrazol.[4]
References
edit- ^ Ashman PJ, Mackenzie A, Bramley PM (November 1990). "Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi". Biochimica et Biophysica Acta (BBA) - General Subjects. 1036 (2): 151–7. doi:10.1016/0304-4165(90)90027-t. PMID 2223832.
- ^ Archer C, Ashman PJ, Hedden P, Bowyer JR, Bramley PM (May 1992). "Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima". Biochemical Society Transactions. 20 (2): 218S. doi:10.1042/bst020218s. PMID 1397591.
- ^ Helliwell CA, Poole A, Peacock WJ, Dennis ES (February 1999). "Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis". Plant Physiology. 119 (2): 507–10. doi:10.1104/pp.119.2.507. PMC 32127. PMID 9952446.
- ^ Davidson, Sandra E.; Smith, Jennifer J.; Helliwell, Chris A.; Poole, Andrew T.; Reid, James B. (2004). "The Pea Gene LH Encodes ent-Kaurene Oxidase". Plant Physiology. 134 (3): 1123–1134. doi:10.1104/pp.103.032706. PMC 389936. PMID 14988475.
External links
edit- Ent-kaurene+oxidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
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