Exodeoxyribonuclease (lambda-induced) (EC 3.1.11.3, lambda exonuclease, phage lambda-induced exonuclease, Escherichia coli exonuclease IV, E. coli exonuclease IV, exodeoxyribonuclease IV, exonuclease IV) is an exonuclease.[1][2] This enzyme catalyses the following chemical reaction
| Exodeoxyribonuclease (lambda-induced) | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.1.11.3 | ||||||||
| CAS no. | 37367-70-7 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- Exonucleolytic cleavage in the 5′- to 3′-direction to yield nucleoside 5′-phosphates
This enzyme has preference for double-stranded DNA (dsDNA). This means that it degrades a single strand of dsDNA, primarily any strand which has a phosphate at its 5' end.[3]
References
edit- ^ Lindahl T, Gally JA, Edelman GM (February 1969). "Deoxyribonuclease IV: a new exonuclease from mammalian tissues". Proceedings of the National Academy of Sciences of the United States of America. 62 (2): 597–603. Bibcode:1969PNAS...62..597L. doi:10.1073/pnas.62.2.597. PMC 277851. PMID 5256235.
- ^ Little JW (February 1967). "An exonuclease induced by bacteriophage lambda. II. Nature of the enzymatic reaction". The Journal of Biological Chemistry. 242 (4): 679–86. doi:10.1016/S0021-9258(18)96258-7. PMID 6017737.
- ^ "Lambda Exonuclease" (PDF). Epicentre. Archived from the original (PDF) on 2014-05-27. Retrieved 2014-05-26.
External links
edit- Exodeoxyribonuclease+(lambda-induced) at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
