In enzymology, a GDP-mannose 3,5-epimerase (EC 5.1.3.18) is an enzyme that catalyzes the chemical reaction
| GDP-mannose 3,5-epimerase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 5.1.3.18 | ||||||||
| CAS no. | 72162-82-4 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
- GDP-mannose GDP-L-galactose + GDP-L-gulose - initial reaction overview (updated in 2020, see below)
Hence, this enzyme has one substrate, GDP-mannose, and two products, GDP-L-galactose and GDP-L-gulose
Since only GDP-L-gulose (the C5-epimer of GDP-D-mannose) was found in the reaction mixture, it was postulated that the enzyme performs the C5-epimerization prior to the C3-epimerization. However, GDP-D-altrose was recently found as a reaction product, which means that both reaction routes can occur: C5-prior-to-C3 and C3-prior-to-C5. This also means that the GDP-mannose 3,5-epimerase has three reaction products, namely the main product GDP-L-galactose (C3,5-epimer) and two sideproducts GDP-L-gulose (C5-epimer) + GDP-D-altrose (C3-epimer).
- GDP-D-mannose GDP-L-galactose (C3,5-epimer) + GDP-L-gulose (C5-epimer) + GDP-D-altrose (C3-epimer) - reaction overview update in 2020
This enzyme belongs to the family of isomerases, specifically those racemases and epimerases acting on carbohydrates and derivatives. The systematic name of this enzyme class is GDP-mannose 3,5-epimerase. Other names in common use include GDP-D-mannose:GDP-L-galactose epimerase, guanosine 5'-diphosphate D-mannose:guanosine 5'-diphosphate, GM35E,[1] and L-galactose epimerase. This enzyme participates in ascorbate and aldarate metabolism.
Structural studies
editAs of late 2007, 4 structures have been solved for this class of enzymes, with PDB accession codes 2C54, 2C59, 2C5A, and 2C5E.
References
edit- ^ Beerens, Koen; Gevaert, Ophelia; Desmet, Tom (2022-01-11). "GDP-Mannose 3,5-Epimerase: A View on Structure, Mechanism, and Industrial Potential". Frontiers in Molecular Biosciences. 8. doi:10.3389/fmolb.2021.784142. ISSN 2296-889X. PMC 8787198. PMID 35087867.
- Barber GA, Hebda PA (1982). "GDP-d-mannose: GDP-l-galactose epimerase from Chlorella pyrenoidosa". Complex Carbohydrates Part D. Methods in Enzymology. Vol. 83. pp. 522–5. doi:10.1016/0076-6879(82)83046-2. ISBN 978-0-12-181983-5. PMID 7098948.
- Hebda PA, Behrman EJ, Barber GA (1979). "The guanosine 5'-diphosphate D-mannose: guanosine 5'-diphosphate L-galactose epimerase of Chlorella pyrenoidosa. Chemical synthesis of guanosine 5'-diphosphate L-galactose and further studies of the enzyme and the reaction it catalyzes". Arch. Biochem. Biophys. 194 (2): 496–502. doi:10.1016/0003-9861(79)90644-1. PMID 443816.
- Gevaert O, Van Overtveldt S, Beerens K, Desmet T (2019). "Characterization of the First Bacterial and Thermostable GDP-Mannose 3,5-Epimerase". Int. J. Mol. Sci. 20 (14): E3530. doi:10.3390/ijms20143530. PMC 6678494. PMID 31330931.
- Gevaert O, Van Overtveldt S, Da Costa M, Beerens K, Desmet T (2020). "GDP-altrose as novel product of GDP-mannose 3,5-epimerase: Revisiting its reaction mechanism". Int J Biol Macromol. 165 (Pt B): 1862–1868. doi:10.1016/j.ijbiomac.2020.10.067. PMID 33075338.
