GLS2

Glutaminase 2 (liver, mitochondrial) is a protein that in humans is encoded by the GLS2 gene.

GLS2
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 4BQM
Identifiers
Aliases	GLS2, GA, GLS, LGA, hLGA, glutaminase 2
External IDs	OMIM: 606365; MGI: 2143539; HomoloGene: 40861; GeneCards: GLS2; OMA:GLS2 - orthologs
Gene location (Human) Chr. Chromosome 12 (human) Band 12q13.3 Start 56,470,944 bp End 56,488,414 bp
Gene location (Mouse) Chr. Chromosome 10 (mouse) Band 10|10 D3 Start 128,030,326 bp End 128,045,873 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right lobe of liver cerebellar hemisphere right hemisphere of cerebellum body of pancreas right frontal lobe oocyte primary visual cortex testicle Brodmann area 9 middle temporal gyrus Top expressed in left lobe of liver lumbar subsegment of spinal cord visual cortex cerebellar cortex anterior horn of spinal cord primary visual cortex facial motor nucleus superior frontal gyrus embryo neural layer of retina More reference expression data BioGPS n/a
Gene ontology Molecular function hydrolase activity protein binding glutaminase activity Cellular component mitochondrial matrix mitochondrion Biological process regulation of apoptotic process glutamate secretion cellular amino acid metabolic process glutamine metabolic process cellular amino acid biosynthetic process glutamate biosynthetic process glutamine catabolic process reactive oxygen species metabolic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 27165 216456 Ensembl ENSG00000135423 ENSMUSG00000044005 UniProt Q9UI32 Q571F8 RefSeq (mRNA) NM_001280796 NM_001280797 NM_001280798 NM_013267 NM_138566 NM_001033264 NM_001285777 NM_001285779 RefSeq (protein) NP_001267725 NP_001267726 NP_001267727 NP_037399 NP_001028436 NP_001272706 NP_001272708 Location (UCSC) Chr 12: 56.47 – 56.49 Mb Chr 10: 128.03 – 128.05 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Structure

The GLS2 gene is on the 12th chromosome in humans, with its specific location being 12q13.3. It contains 19 exons.

Function

GLS2 is a part of the glutaminase family. The protein encoded by this gene is a mitochondrial phosphate-activated glutaminase that catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Originally thought to be liver-specific, this protein has been found in other tissues as well. Alternative splicing results in multiple transcript variants that encode different isoforms.

Clinical significance

GLS2 has interesting molecular relationships with tumor progression and cancer. Glutaminase 2 negatively regulates the PI3K/AKT signaling and shows tumor suppression activity in human hepatocellular carcinoma. Additionally, silencing of GLS and overexpression of GLS2 genes cooperate in decreasing the proliferation and viability of glioblastoma cells.

Further reading

• Suhre K, Shin SY, Petersen AK, Mohney RP, Meredith D, Wägele B, Altmaier E, Deloukas P, Erdmann J, Grundberg E, Hammond CJ, de Angelis MH, Kastenmüller G, Köttgen A, Kronenberg F, Mangino M, Meisinger C, Meitinger T, Mewes HW, Milburn MV, Prehn C, Raffler J, Ried JS, Römisch-Margl W, Samani NJ, Small KS, Wichmann HE, Zhai G, Illig T, Spector TD, Adamski J, Soranzo N, Gieger C (Sep 2011). "Human metabolic individuality in biomedical and pharmaceutical research". Nature. 477 (7362): 54–60. Bibcode:2011Natur.477...54S. doi:10.1038/nature10354. PMC 3832838. PMID 21886157.
• Xiang L, Xie G, Liu C, Zhou J, Chen J, Yu S, Li J, Pang X, Shi H, Liang H (Dec 2013). "Knock-down of glutaminase 2 expression decreases glutathione, NADH, and sensitizes cervical cancer to ionizing radiation". Biochimica et Biophysica Acta (BBA) - Molecular Cell Research. 1833 (12): 2996–3005. doi:10.1016/j.bbamcr.2013.08.003. PMID 23954443.
• Hu W, Zhang C, Wu R, Sun Y, Levine A, Feng Z (Apr 2010). "Glutaminase 2, a novel p53 target gene regulating energy metabolism and antioxidant function". Proceedings of the National Academy of Sciences of the United States of America. 107 (16): 7455–60. Bibcode:2010PNAS..107.7455H. doi:10.1073/pnas.1001006107. PMC 2867677. PMID 20378837.
• Olalla L, Gutiérrez A, Campos JA, Khan ZU, Alonso FJ, Segura JA, Márquez J, Aledo JC (Oct 2002). "Nuclear localization of L-type glutaminase in mammalian brain". The Journal of Biological Chemistry. 277 (41): 38939–44. doi:10.1074/jbc.C200373200. PMID 12163477.
• Cheng CY, Schache M, Ikram MK, Young TL, Guggenheim JA, Vitart V, MacGregor S, Verhoeven VJ, Barathi VA, Liao J, Hysi PG, Bailey-Wilson JE, St Pourcain B, Kemp JP, McMahon G, Timpson NJ, Evans DM, Montgomery GW, Mishra A, Wang YX, Wang JJ, Rochtchina E, Polasek O, Wright AF, Amin N, van Leeuwen EM, Wilson JF, Pennell CE, van Duijn CM, de Jong PT, Vingerling JR, Zhou X, Chen P, Li R, Tay WT, Zheng Y, Chew M, Burdon KP, Craig JE, Iyengar SK, Igo RP, Lass JH, Chew EY, Haller T, Mihailov E, Metspalu A, Wedenoja J, Simpson CL, Wojciechowski R, Höhn R, Mirshahi A, Zeller T, Pfeiffer N, Lackner KJ, Bettecken T, Meitinger T, Oexle K, Pirastu M, Portas L, Nag A, Williams KM, Yonova-Doing E, Klein R, Klein BE, Hosseini SM, Paterson AD, Makela KM, Lehtimaki T, Kahonen M, Raitakari O, Yoshimura N, Matsuda F, Chen LJ, Pang CP, Yip SP, Yap MK, Meguro A, Mizuki N, Inoko H, Foster PJ, Zhao JH, Vithana E, Tai ES, Fan Q, Xu L, Campbell H, Fleck B, Rudan I, Aung T, Hofman A, Uitterlinden AG, Bencic G, Khor CC, Forward H, Pärssinen O, Mitchell P, Rivadeneira F, Hewitt AW, Williams C, Oostra BA, Teo YY, Hammond CJ, Stambolian D, Mackey DA, Klaver CC, Wong TY, Saw SM, Baird PN (Aug 2013). "Nine loci for ocular axial length identified through genome-wide association studies, including shared loci with refractive error". American Journal of Human Genetics. 93 (2): 264–77. doi:10.1016/j.ajhg.2013.06.016. PMC 3772747. PMID 24144296.
• Maeshima H, Ohnuma T, Sakai Y, Shibata N, Baba H, Ihara H, Higashi M, Ohkubo T, Nozawa E, Abe S, Ichikawa A, Nakano Y, Utsumi Y, Suzuki T, Arai H (Oct 2007). "Increased plasma glutamate by antipsychotic medication and its relationship to glutaminase 1 and 2 genotypes in schizophrenia -- Juntendo University Schizophrenia Projects (JUSP)". Progress in Neuro-Psychopharmacology & Biological Psychiatry. 31 (7): 1410–8. doi:10.1016/j.pnpbp.2007.06.009. PMID 17669570. S2CID 22744937.
• Kettunen J, Tukiainen T, Sarin AP, Ortega-Alonso A, Tikkanen E, Lyytikäinen LP, Kangas AJ, Soininen P, Würtz P, Silander K, Dick DM, Rose RJ, Savolainen MJ, Viikari J, Kähönen M, Lehtimäki T, Pietiläinen KH, Inouye M, McCarthy MI, Jula A, Eriksson J, Raitakari OT, Salomaa V, Kaprio J, Järvelin MR, Peltonen L, Perola M, Freimer NB, Ala-Korpela M, Palotie A, Ripatti S (Mar 2012). "Genome-wide association study identifies multiple loci influencing human serum metabolite levels". Nature Genetics. 44 (3): 269–76. doi:10.1038/ng.1073. PMC 3605033. PMID 22286219.
• Szeliga M, Zgrzywa A, Obara-Michlewska M, Albrecht J (Nov 2012). "Transfection of a human glioblastoma cell line with liver-type glutaminase (LGA) down-regulates the expression of DNA-repair gene MGMT and sensitizes the cells to alkylating agents". Journal of Neurochemistry. 123 (3): 428–36. doi:10.1111/j.1471-4159.2012.07917.x. PMID 22888977. S2CID 205624752.
• Pérez-Gómez C, Campos-Sandoval JA, Alonso FJ, Segura JA, Manzanares E, Ruiz-Sánchez P, González ME, Márquez J, Matés JM (Mar 2005). "Co-expression of glutaminase K and L isoenzymes in human tumour cells". The Biochemical Journal. 386 (Pt 3): 535–42. doi:10.1042/BJ20040996. PMC 1134872. PMID 15496140.

This article incorporates text from the United States National Library of Medicine, which is in the public domain.