Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase
Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase (EC 2.4.1.288, GlfT2) is an enzyme with systematic name UDP-alpha-D-galactofuranose:beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 4-beta/5-beta-D-galactofuranosyltransferase.[1][2][3] This enzyme catalyses the following chemical reaction
| Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol beta-1,5/1,6-galactofuranosyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.4.1.288 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- 28 UDP-alpha-D-galactofuranose + beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 28 UDP + [beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->6)]14-beta-D-galactofuranosyl-(1->5)-beta-D-galactofuranosyl-(1->4)-alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
This enzyme is isolated from Mycobacterium tuberculosis.
References
edit- ^ Rose NL, Zheng RB, Pearcey J, Zhou R, Completo GC, Lowary TL (August 2008). "Development of a coupled spectrophotometric assay for GlfT2, a bifunctional mycobacterial galactofuranosyltransferase". Carbohydrate Research. 343 (12): 2130–9. doi:10.1016/j.carres.2008.03.023. PMID 18423586.
- ^ May JF, Splain RA, Brotschi C, Kiessling LL (July 2009). "A tethering mechanism for length control in a processive carbohydrate polymerization". Proceedings of the National Academy of Sciences of the United States of America. 106 (29): 11851–6. Bibcode:2009PNAS..10611851M. doi:10.1073/pnas.0901407106. PMC 2715488. PMID 19571009.
- ^ Wheatley RW, Zheng RB, Richards MR, Lowary TL, Ng KK (August 2012). "Tetrameric structure of the GlfT2 galactofuranosyltransferase reveals a scaffold for the assembly of mycobacterial Arabinogalactan". The Journal of Biological Chemistry. 287 (33): 28132–43. doi:10.1074/jbc.M112.347484. PMC 3431634. PMID 22707726.
External links
edit- Galactofuranosylgalactofuranosylrhamnosyl-N-acetylglucosaminyl-diphospho-decaprenol+beta-1,5/1,6-galactofuranosyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
