The Ghislieri, or less commonly Ghisleri, were an ancient Bolognese aristocratic family :
- Ghislieri
- Michele Ghislieri (1504–1572), also known as Pope Pius V.
- Ghislieri College
- Ghislieri Choir and Consort, Giulio Prandi
- Ghisleri
- Arcangelo Ghisleri (1855–1938), an Italian journalist.