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Glucose 6-phosphatase

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Boron, Walter F.; Boulpaep, Emile L., eds. (2017). Medical Physiology (3rd ed.). Philadelphia, PA: Elsevier. ISBN 978-1-4557-4377-3.

Not to be confused with Glucose-6-phosphate dehydrogenase.
Glucose 6-phosphatase.
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EC no.3.1.3.9
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Glucose-6-phosphate
Glucose

The enzyme glucose 6-phosphatase (EC 3.1.3.9, G6Pase; systematic name D-glucose-6-phosphate phosphohydrolase) catalyzes the hydrolysis of glucose 6-phosphate, resulting in the creation of a phosphate group and free glucose:

D-glucose 6-phosphate + H2O = D-glucose + phosphate

During fasting, adequate levels of blood glucose are assured by glucose liberated from liver glycogen stores by glycogenolysis as well as glucose generated by gluconeogenesis in the liver as well as - to a lesser extent - the kidneys. G6P is the product of both these pathways[1] and must be converted to glucose before it can be exported from the cell into blood by membrane-bound glucose transporters.[2] G6Pase is therefore principally expressed in the liver and kidney[1] - while skeletal muscle collectively contain the most substantial glycogen reserve in the body, glucose cannot be mobilised from it as muscle lacks G6Pase.[3]: 1171 

Insulin inhibits hepatic G6Pase activity,[3]: 1046  whereas glucagon promotes it.[3]: 1052  Expression of G6Pase is increased during starvation, in diabetes, and by glucocorticosteroids.[1]

Glucose 6-phosphatase is a complex of multiple component proteins, including transporters for G6P, glucose, and phosphate. The main phosphatase function is performed by the glucose 6-phosphatase catalytic subunit. In humans, there are three isozymes of the catalytic subunit: glucose 6-phosphatase-α, encoded by G6PC; IGRP, encoded by G6PC2; and glucose 6-phosphatase-β, encoded by G6PC3.[4]

Glucose 6-phosphatase-α and glucose 6-phosphatase-β are both functional phosphohydrolases, and have similar active site structure, topology, mechanism of action, and kinetic properties with respect to G6P hydrolysis.[5] In contrast, IGRP has almost no hydrolase activity, and may play a different role in stimulating pancreatic insulin secretion.[6]

Vanadium containing chloroperoxidase enzyme with amino acid residues shown in color. Vanadium containing chloroperoxidase has a similar structure and active site as glucose 6-phosphatase.(From pdb 1IDQ)
Position of active site amino acid residues of vanadium containing chloroperoxidase shown in relation to enzyme surface.(From pdb 1IDQ)
The active site of vanadium containing chloroperoxidase. The residues Lys353, Arg360, Arg490, His404, and His496 correspond to Lys76, Arg83, Arg170, His119, and His176 in Glc 6-Pase. (From pdb 1IDQ)

Biochemistry

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Although a clear consensus has not been reached, a large number of scientists adhere to a substrate-transport model to account for the catalytic properties of glucose 6-phosphatase. In this model, glucose 6-phosphatase has a low degree of selectivity. The transfer of the glucose 6-phosphate is carried out by a transporter protein (T1) and the endoplasmic reticulum (ER) contains structures allowing the exit of the phosphate group (T2) and glucose (T3).[7]

Glucose 6-phosphatase consists of 357 amino acids, and is anchored to the endoplasmic reticulum (ER) by nine transmembrane helices. Its N-terminal and active site are found on the lumen side of the ER and its C-terminus projects into the cytoplasm. Due to its tight association to the ER, the exact structure of glucose 6-phosphatase remains unknown. However, sequence alignment has shown that glucose 6-phosphatase is structurally similar to the active site of the vanadium-containing chloroperoxidase found in Curvularia inaequalis.[8]

Based on pH kinetic studies of glucose 6-phosphatase-α catalysis, it was proposed that the hydrolysis of glucose 6-phosphate was completed via a covalent phosphohistidine glucose 6-phosphate intermediate. The active site of glucose 6-phosphatase-α was initially identified by the presence of a conserved phosphate signature motif usually found in lipid phosphatases, acid phosphatases, and vanadium haloperoxidases.[5]

Essential residues in the active site of vanadium haloperoxidases include: Lys353, Arg360, Arg490, His404, and His496. Corresponding residues in the active site of glucose 6-phosphatase-α include Arg170 and Arg83, which donate hydrogen ions to the phosphate, stabilizing the transition state, His119, which provides a proton to the dephosphorylated oxygen attached to glucose, and His176, which completes a nucleophilic attack on the phosphate to form a covalently bound phosphoryl enzyme intermediate.[9] Within the Vanadium-containing chloroperoxidase, Lys353 was found to stabilize the phosphate in the transition state. However, the corresponding residue in glucose 6-phosphatase-α (Lys76) resides within the ER membrane and its function, if any, is currently undetermined. With the exception of Lys76, these residues are all located on the luminal side of the ER membrane.[5]

Glucose 6-phosphatase-β is a ubiquitously expressed, 346-amino acid membrane protein that shares 36% sequence identity with glucose 6-phosphatase-α. Within the glucose 6-phosphatase-β enzyme, sequence alignments predict that its active site contains His167, His114, and Arg79. Similar to that of the glucose 6-phosphatase-α active site, His167 is the residue that provides the nucleophilic attack, and His114, and Arg79 are the hydrogen donors. Glucose 6-phosphatase-β is also localized in the ER membrane, although its orientation is unknown.[5]

Mechanism

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The hydrolysis of glucose 6-phosphate begins with a nucleophilic attack on the sugar-bound phosphate by His176 resulting in the formation of a phosphohistidine bond and the degradation of a carbonyl. A Negatively charged oxygen then transfers its electrons reforming a carbonyl and breaking its bond with glucose. The negatively charged glucose-bound oxygen is then protonated by His119 forming a free glucose. The phospho-intermediate produced by the reaction between His176 and the phosphate group is then broken by a hydrophilic attack; after the addition of another hydroxide and the decomposition of a carbonyl, the carbonyl is reformed kicking off the electrons originally donated by the His176 residue thereby creating a free phosphate group and completing the hydrolysis.[9]

 

Expression

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Genes coding for the enzyme are primarily expressed in the liver, in the kidney cortex and (to a lesser extent) in the β-cells of the pancreatic islets and intestinal mucosa (especially during times of starvation).[7] Glucose 6-phosphatase is present in a wide variety of muscles across the animal kingdom, albeit at very low concentrations.[10] Thus, the glycogen that muscles store is not usually available for the rest of the body's cells because glucose 6-phosphate cannot cross the sarcolemma unless it is dephosphorylated. The enzyme plays an important role during periods of fasting and when glucose levels are low. It has been shown that starvation and diabetes induces a two- to threefold increase in glucose 6-phosphatase activity in the liver.[7] Glc 6-Pase activity also increases dramatically at birth when an organism becomes independent of the mothers source of glucose. The human Glc 6-Pase gene contains five exons spanning approximately 125.5 kb DNA located on chromosome 17q21.[11]

Clinical significance

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Mutations of the glucose 6-phosphatase system, to be specific the glucose 6-phosphatase-α subunit (glucose 6-phosphatase-α), glucose 6-transporter (G6PT), and glucose 6-phosphatase-β (glucose 6-phosphatase-β or G6PC3) subunits lead to deficiencies in the maintenance of interprandial glucose homeostasis and neutrophil function and homeostasis.[12][13] Mutations in both glucose 6-phosphatase-α and G6PT lead to glycogen storage disease type I (GSD 1, von Gierke's disease).[14] To be specific, mutations in the glucose-6-phosphatase-α lead to Glycogen Storage Disease Type-1a, which is characterized by accumulation of glycogen and fat in the liver and kidneys, resulting in hepatomegaly and renomegaly.[15] GSD-1a constitutes approximately 80% of GSD-1 cases that present clinically.[16] Absence of G6PT leads to GSD-1b (GSD-1b), which is characterized by the lack of a G6PT and represents 20% of the cases that present clinically.[16][17]

 
Breakdown of the various constituents of glucose 6-phosphatase system deficiency

The specific cause of the GSD-1a stems from nonsense mutations, insertions/deletions with or without a shift in the reading frame, or splice site mutations that occur at the genetic level.[7] The missense mutations affect the two large luminal loops and transmembrane helices of glucose 6-phosphatase-α, abolishing or greatly reducing activity of the enzyme.[7] The specific cause of GSD-1b stems from "severe" mutations such as splice site mutations, frame-shifting mutations, and substitutions of a highly conserved residue that completely destroyed G6PT activity.[7] These mutations lead to the prevalence of GSD-1 by preventing the transport of glucose-6-phosphate (G6P) into the luminal portion of the ER and also inhibiting the conversion of G6P into glucose to be used by the cell.

The third type of glucose 6-phosphatase deficiency, glucose 6-phosphatase-β deficiency, is characterized by a congenital neutropenia syndrome in which neutrophils exhibit enhanced endoplasmic reticulum (ER) stress, increased apoptosis, impaired energy homeostasis, and impaired functionality.[18] It can also lead to cardiac and urogenital malformations.[19] This third class of deficiency is also affected by a G6PT deficiency as glucose-6-phosphatase-β also lies within the ER lumen and thus can lead to similar symptoms of glucose-6-phosphatase-β deficiency be associated with GSD-1b.[17] Furthermore, recent studies have elucidated this area of similarity between both deficiencies and have shown that aberrant glycosylation occurs in both deficiencies.[20] The neutrophil glycosylation has a profound effect on neutrophil activity and thus may also be classified as a congenital glycosylation disorder as well.[20]

The major function of glucose 6-phosphatase-β has been determined to provide recycled glucose to the cytoplasm of neutrophils in order maintain normal function. Disruption of the glucose to G6P ratio due to significant decrease intracellular glucose levels cause significant disruption of glycolysis and HMS.[13] Unless countered by uptake of extracellular glucose this deficiency leads to neutrophil dysfunction.[13]

Vanadium compounds such as vanadyl sulfate have been shown to inhibit the enzyme, and thus increase the insulin sensitivity in vivo in diabetics, as assessed by the hyperinsulinemic clamp technique, which may have potential therapeutic implications.[21][22]

See also

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Notes

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Molecular graphics images were produced using UCSF Chimera.[23]

References

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  1. ^ a b c Van SCHAFTINGEN, Emile; Gerin, Isabelle (15 March 2002). "The glucose-6-phosphatase system". Biochemical Journal. 362 (3): 513–532. doi:10.1042/0264-6021:3620513. PMC 1222414. PMID 11879177.
  2. ^ Nordlie R, et al. (1985). The Enzymes of biological membranes, 2nd edition. New York: Plenum Press. pp. 349–398. ISBN 0-306-41453-8.
  3. ^ a b c Boron, Walter F.; Boulpaep, Emile L., eds. (2017). Medical Physiology (3rd ed.). Philadelphia, PA: Elsevier. ISBN 978-1-4557-4377-3.
  4. ^ Hutton JC, O'Brien RM (October 2009). "Glucose-6-phosphatase catalytic subunit gene family". The Journal of Biological Chemistry. 284 (43): 29241–5. doi:10.1074/jbc.R109.025544. PMC 2785553. PMID 19700406.
  5. ^ a b c d Ghosh A, Shieh JJ, Pan CJ, Chou JY (March 2004). "Histidine 167 is the phosphate acceptor in glucose-6-phosphatase-β forming a phosphohistidine enzyme intermediate during catalysis". The Journal of Biological Chemistry. 279 (13): 12479–83. doi:10.1074/jbc.M313271200. PMID 14718531.
  6. ^ Shieh JJ, Pan CJ, Mansfield BC, Chou JY (September 2005). "In islet-specific glucose-6-phosphatase-related protein, the β cell antigenic sequence that is targeted in diabetes is not responsible for the loss of phosphohydrolase activity". Diabetologia. 48 (9): 1851–9. doi:10.1007/s00125-005-1848-6. PMID 16012821.
  7. ^ a b c d e f van Schaftingen E, Gerin I (March 2002). "The glucose-6-phosphatase system". The Biochemical Journal. 362 (Pt 3): 513–32. doi:10.1042/0264-6021:3620513. PMC 1222414. PMID 11879177.
  8. ^ Pan CJ, Lei KJ, Annabi B, Hemrika W, Chou JY (March 1998). "Transmembrane topology of glucose-6-phosphatase". The Journal of Biological Chemistry. 273 (11): 6144–8. doi:10.1074/jbc.273.11.6144. PMID 9497333.
  9. ^ a b Ghosh A, Shieh JJ, Pan CJ, Sun MS, Chou JY (September 2002). "The catalytic center of glucose-6-phosphatase. HIS176 is the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis". The Journal of Biological Chemistry. 277 (36): 32837–42. doi:10.1074/jbc.M201853200. PMID 12093795.
  10. ^ Surholt, B; Newsholme, EA (15 September 1981). "Maximum activities and properties of glucose 6-phosphatase in muscles from vertebrates and invertebrates". The Biochemical Journal. 198 (3): 621–9. doi:10.1042/bj1980621. PMC 1163310. PMID 6275855.
  11. ^ Angaroni CJ, de Kremer RD, Argaraña CE, Paschini-Capra AE, Giner-Ayala AN, Pezza RJ, Pan CJ, Chou JY (November 2004). "Glycogen storage disease type Ia in Argentina: two novel glucose-6-phosphatase mutations affecting protein stability". Molecular Genetics and Metabolism. 83 (3): 276–9. doi:10.1016/j.ymgme.2004.06.010. PMID 15542400.
  12. ^ Chou JY, Jun HS, Mansfield BC (December 2010). "Glycogen storage disease type I and glucose-6-phosphatase-β deficiency: etiology and therapy". Nature Reviews. Endocrinology. 6 (12): 676–88. doi:10.1038/nrendo.2010.189. PMC 4178929. PMID 20975743.
  13. ^ a b c Jun HS, Lee YM, Cheung YY, McDermott DH, Murphy PM, De Ravin SS, Mansfield BC, Chou JY (October 2010). "Lack of glucose recycling between endoplasmic reticulum and cytoplasm underlies cellular dysfunction in glucose-6-phosphatase-β-deficient neutrophils in a congenital neutropenia syndrome". Blood. 116 (15): 2783–92. doi:10.1182/blood-2009-12-258491. PMC 2974586. PMID 20498302.
  14. ^ Stryer, Lubert; Berg, Jeremy Mark; Tymoczko, John L. (2007). Biochemistry. San Francisco: W.H. Freeman. ISBN 978-0-7167-8724-2.
  15. ^ Pagon RA, Bird TD, Dolan CR, et al. (1993). "Glycogen Storage Disease Type I". PMID 20301489. {{cite journal}}: Cite journal requires |journal= (help)
  16. ^ a b Chou JY, Matern D, Mansfield BC, Chen YT (March 2002). "Type I glycogen storage diseases: disorders of the glucose-6-phosphatase complex". Current Molecular Medicine. 2 (2): 121–43. doi:10.2174/1566524024605798. PMID 11949931.
  17. ^ a b Froissart R, Piraud M, Boudjemline AM, Vianey-Saban C, Petit F, Hubert-Buron A, Eberschweiler PT, Gajdos V, Labrune P (2011). "Glucose-6-phosphatase deficiency". Orphanet Journal of Rare Diseases. 6: 27. doi:10.1186/1750-1172-6-27. PMC 3118311. PMID 21599942.
  18. ^ Jun HS, Lee YM, Song KD, Mansfield BC, Chou JY (April 2011). "G-CSF improves murine G6PC3-deficient neutrophil function by modulating apoptosis and energy homeostasis". Blood. 117 (14): 3881–92. doi:10.1182/blood-2010-08-302059. PMC 3083300. PMID 21292774.
  19. ^ Boztug K, Appaswamy G, Ashikov A, Schäffer AA, Salzer U, Diestelhorst J, Germeshausen M, Brandes G, Lee-Gossler J, Noyan F, Gatzke AK, Minkov M, Greil J, Kratz C, Petropoulou T, Pellier I, Bellanné-Chantelot C, Rezaei N, Mönkemöller K, Irani-Hakimeh N, Bakker H, Gerardy-Schahn R, Zeidler C, Grimbacher B, Welte K, Klein C (January 2009). "A syndrome with congenital neutropenia and mutations in G6PC3". The New England Journal of Medicine. 360 (1): 32–43. doi:10.1056/NEJMoa0805051. PMC 2778311. PMID 19118303.
  20. ^ a b Hayee B, Antonopoulos A, Murphy EJ, Rahman FZ, Sewell G, Smith BN, McCartney S, Furman M, Hall G, Bloom SL, Haslam SM, Morris HR, Boztug K, Klein C, Winchester B, Pick E, Linch DC, Gale RE, Smith AM, Dell A, Segal AW (July 2011). "G6PC3 mutations are associated with a major defect of glycosylation: a novel mechanism for neutrophil dysfunction". Glycobiology. 21 (7): 914–24. doi:10.1093/glycob/cwr023. PMC 3110488. PMID 21385794.
  21. ^ "Effects of vanadyl sulfate on carbohydrate and lipid metabolism in patients with non—insulin-dependent diabetes mellitus - Metabolism - Clinical and Experimental". www.metabolismjournal.com. Retrieved 16 June 2015.
  22. ^ Shehzad, Saima (1 January 2013). "The potential effect of vanadium compounds on glucose-6-phosphatase". Bioscience Horizons. 6: hzt002. doi:10.1093/biohorizons/hzt002. ISSN 1754-7431.
  23. ^ Pettersen EF, Goddard TD, Huang CC, Couch GS, Greenblatt DM, Meng EC, Ferrin TE (October 2004). "UCSF Chimera--a visualization system for exploratory research and analysis" (PDF). Journal of Computational Chemistry. 25 (13): 1605–12. doi:10.1002/jcc.20084. PMID 15264254. S2CID 8747218.
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