In molecular biology, glycoside hydrolase family 45 is a family of glycoside hydrolases.
| Glycosyl hydrolase family 45 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 endoglucanase from humicola insolens at 1.7a resolution | |||||||||
| Identifiers | |||||||||
| Symbol | Glyco_hydro_45 | ||||||||
| Pfam | PF02015 | ||||||||
| Pfam clan | CL0199 | ||||||||
| InterPro | IPR000334 | ||||||||
| PROSITE | PDOC00877 | ||||||||
| SCOP2 | 2eng / SCOPe / SUPFAM | ||||||||
| CAZy | GH45 | ||||||||
| |||||||||
Glycoside hydrolases EC 3.2.1. are a widespread group of enzymes that hydrolyse the glycosidic bond between two or more carbohydrates, or between a carbohydrate and a non-carbohydrate moiety. A classification system for glycoside hydrolases, based on sequence similarity, has led to the definition of >100 different families.[1][2][3] This classification is available on the CAZy web site,[4][5] and also discussed at CAZypedia, an online encyclopedia of carbohydrate active enzymes.[6][7]
Glycoside hydrolase family 45 CAZY GH_45 comprises enzymes with only one known activity; endoglucanase (EC 3.2.1.4). This family is also known as cellulase family K.[8] The best conserved region in these enzymes is located in the N-terminal section. It contains an aspartic acid residue which has been shown[9] to act as a nucleophile in the catalytic mechanism. This also has several cysteines that are involved in forming disulphide bridges.
References
edit- ^ Henrissat B, Callebaut I, Fabrega S, Lehn P, Mornon JP, Davies G (July 1995). "Conserved catalytic machinery and the prediction of a common fold for several families of glycosyl hydrolases". Proceedings of the National Academy of Sciences of the United States of America. 92 (15): 7090–4. Bibcode:1995PNAS...92.7090H. doi:10.1073/pnas.92.15.7090. PMC 41477. PMID 7624375.
- ^ Davies G, Henrissat B (September 1995). "Structures and mechanisms of glycosyl hydrolases". Structure. 3 (9): 853–9. doi:10.1016/S0969-2126(01)00220-9. PMID 8535779.
- ^ Henrissat B, Bairoch A (June 1996). "Updating the sequence-based classification of glycosyl hydrolases". The Biochemical Journal. 316 ( Pt 2) (Pt 2): 695–6. doi:10.1042/bj3160695. PMC 1217404. PMID 8687420.
- ^ "Home". CAZy.org. Retrieved 2018-03-06.
- ^ Lombard V, Golaconda Ramulu H, Drula E, Coutinho PM, Henrissat B (January 2014). "The carbohydrate-active enzymes database (CAZy) in 2013". Nucleic Acids Research. 42 (Database issue): D490-5. doi:10.1093/nar/gkt1178. PMC 3965031. PMID 24270786.
- ^ "Glycoside Hydrolase Family 45". CAZypedia.org. Retrieved 2018-03-06.
- ^ CAZypedia Consortium (December 2018). "Ten years of CAZypedia: a living encyclopedia of carbohydrate-active enzymes" (PDF). Glycobiology. 28 (1): 3–8. doi:10.1093/glycob/cwx089. hdl:21.11116/0000-0003-B7EB-6. PMID 29040563.
- ^ Henrissat B, Bairoch A (August 1993). "New families in the classification of glycosyl hydrolases based on amino acid sequence similarities". The Biochemical Journal. 293 ( Pt 3) (3): 781–8. doi:10.1042/bj2930781. PMC 1134435. PMID 8352747.
- ^ Davies GJ, Dodson GG, Hubbard RE, Tolley SP, Dauter Z, Wilson KS, Hjort C, Mikkelsen JM, Rasmussen G, Schülein M (September 1993). "Structure and function of endoglucanase V". Nature. 365 (6444): 362–4. Bibcode:1993Natur.365..362D. doi:10.1038/365362a0. PMID 8377830. S2CID 4277867.
