The gyrA RNA motif is a conserved RNA structure identified by bioinformatics.[1] The RNAs are present in multiple species of bacteria within the order Pseudomonadales. This order contains the genus Pseudomonas, which includes the opportunistic human pathogen Pseudomonas aeruginosa and Pseudomonas syringae, a plant pathogen.
| gyrA RNA motif | |
|---|---|
 Consensus secondary structure of gyrA RNAs | |
| Identifiers | |
| Symbol | gyrA RNA |
| Rfam | RF01740 |
| Other data | |
| RNA type | Cis-regulatory element |
| Domain(s) | Pseudomonadales |
| PDB structures | PDBe |
gyrA RNAs are always found in the presumed 5' untranslated regions of gyrA genes, which encodes a protein forming a subunit of a DNA gyrase. Resistance to the antibiotic ciprofloxacin in Pseudomonas is often achieved via mutations in the gyrA gene.[2] Because of its positioning, the gyrA RNA motif was hypothesized to be a cis-regulatory element acting up the downstream gyrA genes. However, gyrA was previously regarded as a gene whose level of expression is consistent in a wide variety of growth conditions.[3]
References
edit- ^ Weinberg Z, Wang JX, Bogue J, et al. (March 2010). "Comparative genomics reveals 104 candidate structured RNAs from bacteria, archaea and their metagenomes". Genome Biol. 11 (3): R31. doi:10.1186/gb-2010-11-3-r31. PMC 2864571. PMID 20230605.
- ^ Bonomo RA, Szabo D (September 2006). "Mechanisms of multidrug resistance in Acinetobacter species and Pseudomonas aeruginosa". Clin. Infect. Dis. 43 (Suppl 2): S49–56. doi:10.1086/504477. PMID 16894515.
- ^ Vencato M, Tian F, Alfano JR, et al. (November 2006). "Bioinformatics-enabled identification of the HrpL regulon and type III secretion system effector proteins of Pseudomonas syringae pv. phaseolicola 1448A". Mol. Plant Microbe Interact. 19 (11): 1193–1206. doi:10.1094/MPMI-19-1193. PMID 17073302.
External links
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