Heterostylum robustum

Heterostylum robustum is a species of bee flies in the family Bombyliidae. It has a nearctic distribution, being found in Canada (specifically Alberta), Mexico, and the USA (mainly in the West). It is a bomber fly, parasitizing bees and wasps by launching its eggs into their nests its ovipositor. It is the principal parasite of the alkali bee Nomia melanderi, an important alfalfa pollinator across Washington, Oregon, Idaho, and Wyoming. Other hosts of the fly include Nomia nevadensis bakeri, Dieunomia triangulifera, Calliopsis anthidia, Nomia scutellaris, and Halictus rubicundus.

Heterostylum robustum
Scientific classification
Domain:	Eukaryota
Kingdom:	Animalia
Phylum:	Arthropoda
Class:	Insecta
Order:	Diptera
Family:	Bombyliidae
Tribe:	Bombyliini
Genus:	Heterostylum
Species:	H. robustum
Binomial name
Heterostylum robustum (Osten Sacken, 1877)
Synonyms
Comastes robustus Osten Sacken, 1877

Life cycle and morphology

Eggs

The eggs of H. robustum are 1.2 mm long, 0.7 mm wide, oval-shaped, and tapered on each end. They have a coating of mucus to which soil particles adhere. In the laboratory, the eggs take between about 8 and 11 days to incubate. Like ground bee eggs, they are laid by adults emerging in spring before the beginning of winter.

Larvae

The development of the larvae of H. robustum can be divided into four stages. First stage larvae are nearly-white planidiums, very active and about 1.6 mm long. They have 3 pairs of thoracic bristles, one long pair of caudal bristles, and 8 pairs of smaller bristles on the head and neck. They move by grabbing the surfaces with their mouth hooks, contacting the ground with their posterior pseudopods and pushing forward. After the first stage they very quickly pass through an inactive second stage and spend a longer time in a soft, helpless third stage until they reach the fourth stage, in which they begin parasitizing the host. As of 1973 it was unknown what they eat in the first three stages before parasitization.

When they begin parasitizing the host, they firmly press their mouths to the host larvae, attach themselves with the maxillae, and penetrate the host's flesh with their minute mouth hooks. Disturbed fourth-stage larvae that have begun feeding will let go if disturbed, but will then immediately reattach themselves or find a new host if necessary; several larvae placed together cannibalize each other. These larvae are much more active than many other parasitic larvae. Larvae in the fourth stage feed for 3 or 4 days and grow to about twice their former size. In some areas (in the Utah area, but not in Oregon) after consuming the first host the larvae will leave the burrow and burrow through the soil until it finds another prepupal bee to parasitize, of which it consumes about half.

H. robustum larvae overwinter underground as bee larvae do; they dig upwards, usually from about 5-10 inches below-ground up to 2-3 inches below-ground. They then dig large, oval-shaped spaces in which to spend the winter. Their burrowing is done by gyrating their abdomens (in the back) to pack dirt behind them, and then gyrating their anterior ends (in the front) to loosen dirt ahead and above them.

Adults

Adult H. robustum

Bee flies begin to emerge about 10 days earlier than bees do, in the spring, and their period overlaps with bees by several weeks. They tunnel to the surface and then immediately break and struggle out of the pupal skin, which usually remains stuck to the ground and can sometimes be used to identify the presence of the parasites. They then crawl to a nearby object and wait for about 10 minutes for their wings to dry. During this period they are particularly vulnerable to predators, including many birds. Once they are able to fly, the adults seek nectar and mate before beginning to lay eggs about two days later. Unlike some other bee flies, H. robustum flies do not touch the ground while they do this.

Adults have unclouded wings, a tan thorax, and a gray and buff pattern on the abdomen, as well as a long proboscis. Older flies lose the majority of the hair on their abdomen and become uniformly dark gray. Males and females are similar except that males are holoptic and females are dichoptic.

Agricultural importance

H. robustum is the principal parasite of the alkali bee Nomia melanderi, an important alfalfa pollinator (as of 1960) across Washington, Oregon, Idaho, and Wyoming, and can destroy large percentages of its larvae. The percentage destroyed varies by region from over 90% in Cache Valley, Utah to less than 5% in some areas near Wapato, Washington, but in general is often between 20% and 40% in many areas. In some areas formerly abundant with alkali bees, populations have been reduced to entirely insignificant levels by the parasite.

At least two factors contribute to N. melanderi's ability to survive despite the high parasitism: every year some bees nest alone instead of in groups, and some bees always nest and lay eggs late, or even in a small "second generation" of bees which occurs in late August.

Distribution

Heterostylum robustum is found in Canada (Alberta), Mexico (Baja California Norte and Sur, Coahuila de Zaragoza, Guerrero, Morelos, Nuevo León, Puebla, Sinaloa, Sonora, Tamaulipas), and the USA (Arizona, California, Colorado, Georgia, Idaho, Kansas, Nevada, New Mexico, Oklahoma, Oregon, Texas, Utah).