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Holubec (feminine Holubcová) is a Czech surname. Notable people with the surname include:
- Aleš Holubec (born 1984), Czech volleyball player
- Jiří Holubec (born 1966), Czech biathlete
- Kateřina Holubcová (born 1976), Czech biathlete
- Tomáš Holubec (born 1976), Czech biathlete