HuS-E/2 cells are immortalized primary human hepatocytes that were isolated from the discarded old liver tissue obtained from a 9-year-old male patient with primary hyperoxaluria (after the approval of ethical committee and required written consent) who had undergone liver transplantation to receive a new liver.[1] Hepatocytes were transduced with hTERT and HPV-E6E7 oncogenes. This immortalized hepatocyte cell line showed similar characteristics to primary hepatocytes, and was beneficial in the study of Hepatitis C and B viruses.[1][2][3][4]
References
edit- ^ a b Aly, Hussein H.; Watashi, Koichi; Hijikata, Makoto; Kaneko, Hiroyasu; Takada, Yasutugu; Egawa, Hiroto; Uemoto, Shinji; Shimotohno, Kunitada (January 2007). "Serum-derived hepatitis C virus infectivity in interferon regulatory factor-7-suppressed human primary hepatocytes" (PDF). Journal of Hepatology. 46 (1): 26–36. doi:10.1016/j.jhep.2006.08.018. hdl:2433/135683. PMID 17112629.
- ^ Aly, Hussein Hassan; Qi, Yue; Atsuzawa, Kimie; Usuda, Nobuteru; Takada, Yasutsugu; Mizokami, Masashi; Shimotohno, Kunitada; Hijikata, Makoto (September 2009). "Strain-dependent viral dynamics and virus-cell interactions in a novel in vitro system supporting the life cycle of blood-borne hepatitis C virus". Hepatology. 50 (3): 689–696. doi:10.1002/hep.23034. PMID 19489071.
- ^ Abe, Yuichi; Aly, Hussein Hassan; Hiraga, Nobuhiko; Imamura, Michio; Wakita, Takaji; Shimotohno, Kunitada; Chayama, Kazuaki; Hijikata, Makoto (September 2013). "Thromboxane A2 Synthase Inhibitors Prevent Production of Infectious Hepatitis C Virus in Mice With Humanized Livers". Gastroenterology. 145 (3): 658–667.e11. doi:10.1053/j.gastro.2013.05.014. hdl:2433/178751. PMID 23684750.
- ^ Huang, Hsiu-Chen; Tao, Mi-Hua; Hung, Tzu-Min; Chen, Jui-Chieh; Lin, Zi-Jun; Huang, Cheng (November 2014). "(−)-Epigallocatechin-3-gallate inhibits entry of hepatitis B virus into hepatocytes". Antiviral Research. 111: 100–111. doi:10.1016/j.antiviral.2014.09.009. PMID 25260897.