I-140 may refer to:
- Interstate 140 (North Carolina), a bypass of Wilmington, North Carolina
- Interstate 140 (Tennessee), a spur route in Knoxville, Tennessee
- Form I-140, Immigrant Petition for Alien Worker, a form that needs to be filed as part of the application process for an employment-based visa for the United States
- Iodine-140 (I-140 or 140I), an isotope of iodine
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