John Lambe (c. 1545–1628) was English astrologer and adviser to George Villiers, 1st Duke of Buckingham.
John Lambe may also refer to:
- Sir John Lambe (Dean of the Arches) (1566?–1647), English cleric
- John Lambe (M5 rapist) (born 1944), English serial rapist between 1975 and 1980
- John Lambe (priest) (1649–1708)