John MacIsaac may refer to:
- John A. MacIsaac (1883–1942), member of the Nova Scotia House of Assembly
- John L. MacIsaac (1870–1941), member of the Nova Scotia House of Assembly
- John MacIsaac (athlete), see 1958 European Athletics Championships – Men's 4 × 400 metres relay
This disambiguation page lists articles about people with the same name.
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