Josefov is a town quarter in Prague, Czech Republic, known as the Jewish quarter.
Josefov may also refer to places in the Czech Republic:
- Josefov (Hodonín District), a municipality and village in the South Moravian Region
- Josefov (Sokolov District), a municipality and village in the Karlovy Vary Region
- Josefov, a town part of Jaroměř in the Hradec Králové Region
- Josefov Fortress, a former fortress in Josefov
- Josefov Meadows, a bird reserve near Josefov
- Josefov, a village and part of Rožná in the Vysočina Region