In molecular biology, the KilA-N domain is a conserved DNA-binding domain found at the N-terminus of the poxvirus D6R/NIR proteins. It is also found in a wide range of proteins of large bacterial and eukaryotic DNA viruses.[1] Putative proteins with homology to the KilA-N domain have also been identified in Maverick transposable elements of the parabasalid protozoa Trichomonas vaginalis.[2] The KilA-N domain has been suggested to be homologous to the fungal DNA-binding APSES domain. In all proteins shown to contain the KilA-N domain, it occurs at the extreme amino terminus accompanied by a wide range of distinct carboxy-terminal domains. These carboxy-terminal modules may be enzymes, such as the nuclease domains, or might mediate additional, specific interactions with nucleic acids or proteins, like the RING or CCCH fingers in the poxviruses.[1] The KilA-N domain is predicted to adopt an alpha-beta fold with four conserved strands and at least two conserved helices.[1] Some proteins known to contain a KilA-N domain are listed below:
- Bacteriophage P1 protein kilA
- Fowlpox virus (FPV) protein FPV236.
- Trichomonas vaginalis G3 Putative uncharacterised protein
- Vaccinia virus hypothetical 21.7 kDa HindIII-C protein
| KilA-N | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 mbp1 from saccharomyces cerevisiae | |||||||||
| Identifiers | |||||||||
| Symbol | KilA-N | ||||||||
| Pfam | PF04383 | ||||||||
| InterPro | IPR018004 | ||||||||
| SCOP2 | 1bm8 / SCOPe / SUPFAM | ||||||||
| |||||||||
References
edit- ^ a b c Iyer LM, Koonin EV, Aravind L (2002). "Extensive domain shuffling in transcription regulators of DNA viruses and implications for the origin of fungal APSES transcription factors". Genome Biol. 3 (3): RESEARCH0012. doi:10.1186/gb-2002-3-3-research0012. PMC 88810. PMID 11897024.
- ^ Pritham EJ, Putliwala T, Feschotte C (April 2007). "Mavericks, a novel class of giant transposable elements widespread in eukaryotes and related to DNA viruses". Gene. 390 (1–2): 3–17. doi:10.1016/j.gene.2006.08.008. PMID 17034960.