List of restriction enzyme cutting sites: O–R

Legend of nucleobases
Code	Nucleotide represented
A	Adenine (A)
C	Cytosine (C)
G	Guanine (G)
T	Thymine (T)
N	A, C, G or T
M	A or C
R	A or G
W	A or T
Y	C or T
S	C or G
K	G or T
H	A, C or T
B	C, G or T
V	A, C or G
D	A, G or T

This article contains a list of the most studied restriction enzymes whose names start with O to R inclusive. It contains approximately 130 enzymes. The following information is given:

Enzyme: Accepted name of the molecule, according to the internationally adopted nomenclature^[1]^[2], and bibliographical references. (Further reading: see the section "Nomenclature" in the article "Restriction enzyme".)
PDB code: Code used to identify the structure of a protein in the PDB database of protein structures. The 3D atomic structure of a protein provides highly valuable information to understand the intimate details of its mechanism of action^[3]^[4].
Source: Organism that naturally produces the enzyme.
Recognition sequence: Sequence of DNA recognized by the enzyme and to which it specifically binds.
Cut: Cutting site and DNA products of the cut. The recognition sequence and the cutting site usually match, but sometimes the cutting site can be dozens of nucleotides away from the recognition site^[5]^[6].
Isoschizomers and neoschizomers: An isoschizomer is an enzyme that recognizes the same sequence as another. A neoschizomer is a special type of isoschizomer that recognizes the same sequence as another, but cuts in a different manner. A maximum number of 8-10 most common isoschizomers are indicated for every enzyme but there may be many more. Neoschizomers are shown in bold and green color font (e.g.: BamHI). When "None on date" is indicated, that means that there were no registered isoschizomers in the databases on that date with a clearly defined cutting site. Isoschizomers indicated in white font and grey background correspond to enzymes not listed in the current lists:

as in this not listed enzyme:

EcoR70I

Whole list navigation

Restriction enzymes

O

Enzyme	PDB code	Source	Recognition sequence	Cut	Isoschizomers
OfoI		Oscillatoria foreaui A-1340	`5' CYCGRG` `3' GRGCYC`	`5' ---C YCGRG--- 3'` `3' ---GRGCY C--- 5'`	AquI, AvaI, Bse15I, BspLU4I, Eco27kI, Nli3877I, PlaAI, PunAI
OkrAI		Oceanospirillum kriegii	`5' GGATCC` `3' CCTAGG`	`5' ---G GATCC--- 3'` `3' ---CCTAG G--- 5'`	BamHI, Bce751I, BnaI, Bsp98I, BstI, OkrAI, SolI, Uba4009I
OliI		Oceanospirillum linum 4-5D	`5' CACN₄GTG` `3' GTGN₄CAC`	`5' ---CACNN NNGTG--- 3'` `3' ---GTGNN NNCAC--- 5'`	AleI
OxaNI		Oerskovia xanthineolytica N	`5' CCTNAGG` `3' GGANTCC`	`5' ---CC TNAGG--- 3'` `3' ---GGANT CC--- 5'`	AocI, BliHKI, BspR7I, CvnI, Lmu60I, OxaNI, SauI, SshAI

P

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

PabI

2DVY

Pyrococcus abyssi

5' GTAC
3' CATG

5' ---GTA C--- 3'
3' ---C ATG--- 5'

AfaI, Csp6I, CviQI, HpyBI,
HpyBI, PlaAII, RsaI,	RsaNI,

PacI Pseudomonas alcaligenes 5' TTAATTAA
3' AATTAATT 5' ---TTAAT TAA--- 3'
3' ---AAT TAATT--- 5'

Pac25I Pseudomonas alcaligenes 5' CCCGGG
3' GGGCCC 5' ---C CCGGG--- 3'
3' ---GGGCC C--- 5' AhyI, Cfr9I, EaeAI, EclRI, PspAI, PspALI, XcyI, XmaI, XmaCI

PaeI Pseudomonas aeruginosa 5' GCATGC
3' CGTACG 5' ---GCATG C--- 3'
3' ---C GTACG--- 5'

PaeAI Pseudomonas aeruginosa 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

PaeBI Pseudomonas aeruginosa - 18 5' CCCGGG
3' GGGCCC 5' ---CCC GGG--- 3'
3' ---GGG CCC--- 5' AhyI, Cfr9I, EaeAI, EclRI, PspALI, SmaI, TspMI, XcyI, XmaI, XmaCI

PaeHI Pseudomonas aeruginosa 4148 5' GRGCYC
3' CYCGRG 5' ---GRGCY C--- 3'
3' ---C YCGRG--- 5'

PaeHII Pseudomonas aeruginosa 4148 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' AliAJI, BspBI, BspMAI, CfuII, Ecl2zI, HalII, Srl5DI, Sst12I, XcpI

PaeQI Pseudomonas aeruginosa Q2 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

PaeR7I Pseudomonas aeruginosa PA0303 pMG7 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' AbrI, BluI, BssHI, MavI, Sau3239I, Sol10179I, StrI, TliI

Pae2kI Pseudomonas aeruginosa 2k 5' AGATCT
3' TCTAGA 5' ---A GATCT--- 3'
3' ---TCTAG A--- 5'

Pae5kI Pseudomonas aeruginosa 5k 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

Pae14kI Pseudomonas aeruginosa 14k 5' CCGCGG
3' GGCGCC 5' ---CCGC GG--- 3'
3' ---GG CGCC--- 5'

Pae17kI Pseudomonas aeruginosa 17k 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

Pae18kI Pseudomonas aeruginosa 18k 5' AGATCT
3' TCTAGA 5' ---A GATCT--- 3'
3' ---TCTAG A--- 5'

PagI Pseudomonas alcaligenes Sau 14-027 5' TCATGA
3' AGTACT 5' ---T CATGA--- 3'
3' ---AGTAC T--- 5'

PalI Providencia alcalifaciens 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

PamI Phormidium ambiguum 5' TGCGCA
3' ACGCGT 5' ---TGC GCA--- 3'
3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FdiII, FspI, MstI, NsbI, Pun14627I

PamII Phormidium ambiguum 5' GRCGYC
3' CYGCRG 5' ---GR CGYC--- 3'
3' ---CYGC RG--- 5' AsuIII, BsaHI, BstACI, HgiDI, HgiGI, HgiHII, Hin1I, Hsp92I, Msp17I

PanI Pseudomonas alkanolytica 5' CTCGAG
3' GAGCTC 5' ---C TCGAG--- 3'
3' ---GAGCT C--- 5' BspAAI, BstVI, MlaAI, PaeR7I, Sbi68I, Sfr274I, SlaI, XpaI

PasI Pseudomonas anguilliseptica RFL1 5' CCCWGGG
3' GGGWCCC 5' ---CC CWGGG--- 3'
3' ---GGGWC CC--- 5' — None on May 2010 —

PauI Paracoccus alcaliphilus ZVK3-3 5' GCGCGC
3' CGCGCG 5' ---G CGCGC--- 3'
3' ---CGCGC G--- 5'

PauAI Phormidium autumnale 5' RCATGY
3' YGTACR 5' ---RCATG Y--- 3'
3' ---Y GTACR--- 5'

PauAII Phormidium autumnale 5' TTTAAA
3' AAATTT 5' ---TTT AAA--- 3'
3' ---AAA TTT--- 5' AhaIII, DraI, SruI

PceI Planococcus citreus 55 5' AGGCCT
3' TCCGGA 5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5' AatI, AspMI, GdiI, PceI, Pme55I, SarI, Sru30DI, SseBI, SteI, StuI

PciI Planococcus citreus SE-F45 5' ACATGT
3' TGTACA 5' ---A CATGT--- 3'
3' ---TGTAC A--- 5'

PctI

Planococcus citreus SM

5' GAATGC
3' CTTACG

5' ---GAATGCN --- 3'
3' ---CTTAC GN--- 5'

Asp26HI, Asp27HI, Asp35HI
BmaHI,	BsaMI, BscCI, Mva1269I

Pde12I Paracoccus denitrificans 12 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AvcI, Bal228I, Bce22I, Bsp1894I, Bsu54I, Cfr13I, NspIV, UnbI

Pde133I Paracoccus denitrificans 133 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

Pde137I Paracoccus denitrificans 137 5' CCGG
3' GGCC 5' ---C CGG--- 3'
3' ---GGC C--- 5'

PdiI Pseudomonas diminuta Mck 33-321 5' GCCGGC
3' CGGCCG 5' ---GCC GGC--- 3'
3' ---CGG CCG--- 5'

PdmI Pseudomonas diminuta Auk 5-324 5' GAAN₄TTC
3' CTTN₄AAG 5' ---GAANN NNTTC--- 3'
3' ---CTTNN NNAAG--- 5' Asp700I, BbvAI, MroXI, XmnI

PfaAI Phormidium favosum 5' GGYRCC
3' CCRYGG 5' ---G GYRCC--- 3'
3' ---CCRYG G--- 5' AccB1I, BanI, BbvBI, BshNI, Eco64I, HgiCI, HgiHI, MspB4I

PfaAII Phormidium favosum 5' CATATG
3' GTATAC 5' ---CA TATG--- 3'
3' ---GTAT AC--- 5'

PfaAIII Phormidium favosum 5' GCATGC
3' CGTACG 5' ---GCATG C--- 3'
3' ---C GTACG--- 5'

PfeI Psychrobacter faecalis RFL1 5' GAWTC
3' CTWAG 5' ---G AWTC--- 3'
3' ---CTWA G--- 5'

Pfl8I Pseudomonas fluorescens 8 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AliAJI, BspBI, BsuBI, CflI, CfrA4I, PaePI, Pfl21I, PstI, SflII, Sst12I

Pfl21I Pseudomonas sp. 21 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5'

Pfl23II Pseudomonas fluorescens RFL23 5' CGTACG
3' GCATGC 5' ---C GTACG--- 3'
3' ---GCATG C--- 5'

Pfl27I Pseudomonas fluorescens RFL27 5' RGGWCCY
3' YCCWGGR 5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'

PflBI Pseudomonas fluorescens type B 5' CCAN₅TGG
3' GGTN₅ACC 5' ---CCANNNN NTGG--- 3'
3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflMI, Van91I

PflFI Pseudomonas fluorescens biotype F 5' GACNNNGTC
3' CTGNNNCAG 5' ---GACN NNGTC--- 3'
3' ---CTGNN NCAG--- 5' AspI, AtsI, PsyI, TelI, Tth111I

PflKI Pseudomonas fluorescens 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

PflMI Pseudomonas fluorescens 5' CCAN₅TGG
3' GGTN₅ACC 5' ---CCANNNN NTGG--- 3'
3' ---GGTN NNNNACC--- 5' AccB7I, AcpII, Asp10HII, BasI, Esp1396I, PflBI, Van91I

PfoI Pseudomonas fluorescens biovar 126 5' TCCNGGA
3' AGGNCCT 5' ---T CCNGGA--- 3'
3' ---AGGNCC T--- 5'

PgaI Pseudomonas gladioli B4 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BanIII, BbvAII, BliAI, BscI, BspJI, BstNZ169I, ClaI, LcaI, Ssp27144I

PhaI Pasteurella haemolytica 5' GCATC
3' CGTAG 5' ---GCATCN₄N NNNN--- 3'
3' ---CGTAGN₄NNNNN --- 5'

PhoI Pyrococcus horikoshii OT3 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

PinAI

Pseudomonas inequalis

5' ACCGGT
3' TGGCCA

5' ---A CCGGT--- 3'
3' ---TGGCC A--- 5'

AgeI, AsiAI,	AsiGI,	BshTI
CsiAI, CspAI

PinBI Phormidium inundatum 5' ATGCAT
3' TACGTA 5' ---ATGCA T--- 3'
3' ---T ACGTA--- 5' EcoT22I, BfrBI, Mph1103I, NsiI, Ppu10I, SepI, SspD5II, Zsp2I

PinBII Phormidium inundatum 5' TCCGGA
3' AGGCCT 5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5' Aor13HI, BlfI, Bsp13I, BspEI, Bsu23I, Kpn2I, PinBII, PtaI

PlaI Phormidium lapideum 5' GGCC
3' CCGG 5' ---GG CC--- 3'
3' ---CC GG--- 5'

PlaII Phormidium lapideum 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' Asp10HI, BimI, Bsp119I, BstBI, Csp68KII, MlaI, SfuI, SspRFI

PlaAI Phormidium laminosum 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' AquI, BcoI, Bse15I, BspLU4I, Eco27kI, Nli3877I, OfoI, PunAI

PlaAII

Phormidium laminosum

5' GTAC
3' CATG

5' ---GT AC--- 3'
3' ---CA TG--- 5'

AfaI, Csp6I, CviQI, CviRII,
HpyBI, PabI, RsaI,	RsaNI,

PleI Pseudomonas lemoignei 5' GAGTC
3' CTCAG 5' ---GAGTCNNNN N--- 3'
3' ---CTCAGNNNNN --- 5'

Ple19I Pseudomonas lemoignei 19 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa22MI, BspCI, ErhB9I, MvrI, Psu161I, PvuI, RshI, XorII

PmaCI Pseudomonas maltophilia CB50P 5' CACGTG
3' GTGCAC 5' ---CAC GTG--- 3'
3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, Eco72I, PmlI, PspCI

PmeI Pseudomonas mendocina 5' GTTTAAAC
3' CAAATTTG 5' ---GTTT AAAC--- 3'
3' ---CAAA TTTG--- 5' MssI

Pme55I Pseudomonas mendocina 55 5' AGGCCT
3' TCCGGA 5' ---AGG CCT--- 3'
3' ---TCC GGA--- 5' AatI, AspMI, Eco147I, GdiI, PceI, SarI, Sru30DI, SseBI, SteI, StuI

PmlI Pseudomonas maltophilia 5' CACGTG
3' GTGCAC 5' ---CAC GTG--- 3'
3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PspCI

PovII Proteus vulgaris 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

PpaAI Phormidium papyraceum 5' TTCGAA
3' AAGCTT 5' ---TT CGAA--- 3'
3' ---AAGC TT--- 5' AsuII, BimI, Bsp119I, CbiI, Csp68KII, SfuI, SspRFI, SviI

PpaAII Phormidium papyraceum 5' TCGA
3' AGCT 5' ---T CGA--- 3'
3' ---AGC T--- 5'

PpeI Phormidium persicinum 5' GGGCCC
3' CCCGGG 5' ---GGGCC C--- 3'
3' ---C CCGGG--- 5' ApaI, Bsp120I, PspOMI

PpiI Pseudomonas putida Jo 4-731 5' GAACN₅CTC
3' CTTGN₅GAG 5' ---GAACN₅CTCN₇NNNNNN --- 3'
3' ---CTTGN₅GAGN₇N NNNNN--- 5'

PpsI Pseudomonas pseudoalcaligenes PL 5' GAGTC
3' CTCAG 5' ---GAGTCNNNN N--- 3'
3' ---CTCAGNNNNN --- 5'

Ppu10I Pseudomonas putida RFL10 5' ATGCAT
3' TACGTA 5' ---A TGCAT--- 3'
3' ---TACGT A--- 5' BfrBI, Csp68KII, EcoT22I, Mph1103I, NsiI, SepI, SspD5II

Ppu111I Pseudomonas putida P111 5' GAATTC
3' CTTAAG 5' ---G AATTC--- 3'
3' ---CTTAA G--- 5'

PpuAI Pseudomonas putida A1 5' CGTACG
3' GCATGC 5' ---C GTACG--- 3'
3' ---GCATG C--- 5'

PpuMI Pseudomonas putida M 5' RGGWCCY
3' YCCWGGR 5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'

PpuXI Pseudomonas putida X 5' RGGWCCY
3' YCCWGGR 5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'

PshAI Plesiomonas shigelloides 319-73 5' GACN₄GTC
3' CTGN₄CAG 5' ---GACNN NNGTC--- 3'
3' ---CTGNN NNCAG--- 5'

PshBI Plesiomonas shigelloides TPS970 5' ATTAAT
3' TAATTA 5' ---AT TAAT--- 3'
3' ---TAAT TA--- 5' AseI, AsnI, BpoAI, Sru4DI, VspI

PsiI Pseudomonas sp. SE-G49 5' TTATAA
3' AATATT 5' ---TTA TAA--- 3'
3' ---AAT ATT--- 5'

Psp03I Phormidium sp. 5' GGWCC
3' CCWGG 5' ---GGWC C--- 3'
3' ---C CWGG--- 5' Bme216I, CauI, EagMI, FdiI, HgiBI, HgiHIII, SinI, VpaK11AI

Psp5II Pseudomonas sp. RFL5 5' RGGWCCY
3' YCCWGGR 5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'

Psp6I Pseudomonas sp. B6 5' CCWGG
3' GGWCC 5' --- CCWGG--- 3'
3' ---GGWCC --- 5' AeuI, AglI, ApaORI, Bse17I, EcoRII, Fsp1604I, SspAI, Sth117I

Psp23I Pseudomonas sp. 23 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' ApiI, BspBI, CflI, MhaAI, Pfl21I, PstI, SalPI, Sst12I, YenI

Psp1406I Pseudomonas pseudoalcaligenes RFL1406 5' AACGTT
3' TTGCAA 5' ---AA CGTT--- 3'
3' ---TTGC AA--- 5' AclI

PspAI Pseudomonas sp. 5' CCCGGG
3' GGGCCC 5' ---C CCGGG--- 3'
3' ---GGGCC C--- 5' AhyI, CfrJ4I, EaeAI, EclRI, Pac25I, SmaI, TspMI, XcyI, XmaI, XmaCI

PspALI Pseudomonas sp. AL1637 5' CCCGGG
3' GGGCCC 5' ---CCC GGG--- 3'
3' ---GGG CCC--- 5' AhyI, Cfr9I, EaeAI, EclRI, PspALI, SmaI, TspMI, XcyI, XmaI, XmaCI

Psp124BI Pseudomonas sp. 124B 5' GAGCTC
3' CTCGAG 5' ---GAGCT C--- 3'
3' ---C TCGAG--- 5'

PspCI Pseudomonas sp. C 5' CACGTG
3' GTGCAC 5' ---CAC GTG--- 3'
3' ---GTG CAC--- 5' AcvI, BcoAI, BbrPI, Eco72I, PmaCI, PmlI

PspEI Pseudomonas sp. E 5' GGTNACC
3' CCANTGG 5' ---G GTNACC--- 3'
3' ---CCANTG G--- 5' AcrII, AspAI, BstEII, BstPI, Eci125I, Eco91I, EcoO65I, EcoO128I

PspGI 3BM3 Pyrococcus sp. GI-H 5' CCWGG
3' GGWCC 5' --- CCWGG--- 3'
3' ---GGWCC --- 5' AeuI, AjnI, AorI, Bse17I, EcoRII, Fsp1604I, Psp6I, SspAI, Sth117I

PspLI Pseudomonas sp. L 5' CGTACG
3' GCATGC 5' ---C GTACG--- 3'
3' ---GCATG C--- 5'

PspN4I

Pseudomonas sp. N4

5' GGNNCC
3' CCNNGG

5' ---GGN NCC--- 3'
3' ---CCN NGG--- 5'

AspNI, BscBI,	BmiI,	BspLI,
NlaIV

PspOMI Pseudomonas sp. OM2164 5' GGGCCC
3' CCCGGG 5' ---G GGCCC--- 3'
3' ---CCCGG G--- 5' ApaI, Bsp120I, PpeI

PspPI Psychrobacter immobilis TA137 5' GGNCC
3' CCNGG 5' ---G GNCC--- 3'
3' ---CCNG G--- 5' AsuI, Bal228I, BavBII, BsiZI, BspF4I, Cfr13I, Nsp7121I, Sau96I

PspPPI Pseudomonas sp. PP 5' RGGWCCY
3' YCCWGGR 5' ---RG GWCCY--- 3'
3' ---YCCWG GR--- 5'

PspXI Pseudomonas sp. A1-1 5' VCTCGAGB
3' BGAGCTCV 5' ---VC TCGAGB--- 3'
3' ---BGAGCT CV--- 5'

PsrI Pseudomonas stutzeri N2 5' GAACN₆TAC
3' CTTGN₆ATG 5' ---GAACN₆TACN₆NNNNNN --- 3'
3' ---CTTGN₆ATGN₆N NNNNN--- 5'

PssI Pseudomonas sp. 5' RGGNCCY
3' YCCNGGR 5' ---RGGNC CY--- 3'
3' ---YC CNGGR--- 5'

PstI^[7] Providencia stuartii 164 5' CTGCAG
3' GACGTC 5' ---CTGCA G--- 3'
3' ---G ACGTC--- 5' AliAJI, BspBI, CfuII, Ecl2zI, HalII, PstI, Sag16I, Sag23I, Sst12I, XcpI

PstNHI

Pseudomonas stutzeri NH

5' GCTAGC
3' CGATCG

5' ---G CTAGC--- 3'
3' ---CGATC G--- 5'

AceII, AsuNHI, BmtI,	BspOI,
NheI, LlaG2I

PsuI Pseudomonas stutzeri Mck 28-pH52 5' RGATCY
3' YCTAGR 5' ---R GATCY--- 3'
3' ---YCTAG R--- 5'

Psu161I Pseudomonas stutzeri 161 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa16RI, BspCI, EagBI, MvrI, Ple19I, PvuI, RshI, XorII

PsuAI Phormidium subfuscum 5' YACGTR
3' RTGCAY 5' ---YAC GTR--- 3'
3' ---RTG CAY--- 5'

PsyI Pseudomonas syringae Lki 1-pH124 5' GACNNNGTC
3' CTGNNNCAG 5' ---GACN NNGTC--- 3'
3' ---CTGNN NCAG--- 5' AspI, AtsI, PflFI, TelI, Tth111I

PtaI Phormidium tadzschicicum 5' TCCGGA
3' AGGCCT 5' ---T CCGGA--- 3'
3' ---AGGCC T--- 5' Aor13HI, BlfI, BseAI, Bsp13I, BspEI, Kpn2I, PinBII, PtaI

Pun14627I Phormidium uncinatum 1462/7 5' TGCGCA
3' ACGCGT 5' ---TGC GCA--- 3'
3' ---ACG CGT--- 5' Acc16I, AosI, AviII, FdiII, FspI, MstI, NsbI, PamI

Pun14627II Phormidium uncinatum 1462/7 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

PunAI Phormidium uncinatum 5' CYCGRG
3' GRGCYC 5' ---C YCGRG--- 3'
3' ---GRGCY C--- 5' AquI, BcoI, BsiHKCI, BspLU4I, Eco27kI, Nli3877I, PlaAI

PunAII Phormidium uncinatum 5' RCATGY
3' YGTACR 5' ---RCATG Y--- 3'
3' ---Y GTACR--- 5'

PvuI Proteus vulgaris 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa22MI, BspCI, ErhB9I, NblI, Ple19I, Psu161I, RshI, XorII

PvuII 1PVU Proteus vulgaris 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

Pvu84II Proteus vulgaris 84 5' CAGCTG
3' GTCGAC 5' ---CAG CTG--- 3'
3' ---GTC GAC--- 5'

R

Enzyme PDB code Source Recognition sequence Cut Isoschizomers

RalF40I Ruminococcus albus F-40 5' GATC
3' CTAG 5' --- GATC--- 3'
3' ---CTAG --- 5' BscFI, BspAI, BstENII, ChaI, HacI, MkrAI, Sau3AI, SsiAI

RcaI Rhodococcus capsulatum 5' TCATGA
3' AGTACT 5' ---T CATGA--- 3'
3' ---AGTAC T--- 5'

RflFI Ruminococcus flavefaciens FD-1 5' GTCGAC
3' CAGCTG 5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'

RflFII

Ruminococcus flavefaciens FD-1

5' AGTACT
3' TCATGA

5' ---AGT ACT--- 3'
3' ---TCA TGA--- 5'

Acc113I, AssI,	BmcAI, Bpa34I,
DpaI, Eco255I, ScaI, ZrmI

RleAI Rhizobium leguminosarum 5' CCCACA
3' GGGTGT 5' ---CCCACAN₈NNNN --- 3'
3' ---GGGTGTN₈N NNN--- 5'

RmaI Rhodothermus marinus 5' CTAG
3' GATC 5' ---C TAG--- 3'
3' ---GAT C--- 5'

Rme21I Rhizobium meliloti 5' ATCGAT
3' TAGCTA 5' ---AT CGAT--- 3'
3' ---TAGC TA--- 5' BanIII, BbvAII, BliRI, BscI, BspJI, BstNZ169I, ClaI, LcaI, Ssp27144I

RsaI

Rhodopseudomonas sphaeroides

5' GTAC
3' CATG

5' ---GT AC--- 3'
3' ---CA TG--- 5'

AfaI, Csp6I, CviQI, CviRII,
HpyBI, PabI, PlaAII,	RsaNI,

RshI Rhodopseudomonas sphaeroides 2.4.1 5' CGATCG
3' GCTAGC 5' ---CGAT CG--- 3'
3' ---GC TAGC--- 5' Afa16RI, BspCI, EagBI, MvrI, Ple19I, Psu161I, PvuI, XorII

RspLKI Rhodococcus sp. LK2 5' GCATGC
3' CGTACG 5' ---GCATG C--- 3'
3' ---C GTACG--- 5'

RspLKII Rhodococcus sp. LK2 5' GGATCC
3' CCTAGG 5' ---G GATCC--- 3'
3' ---CCTAG G--- 5' AliI, AsiI, BamHI, Bce751I, Nsp29132II, OkrAI, RspLKII, SolI

RspXI Rhodococcus sp. 5' TCATGA
3' AGTACT 5' ---T CATGA--- 3'
3' ---AGTAC T--- 5'

RsrI Rhodopseudomonas sphaeroides 5' GAATTC
3' CTTAAG 5' ---G AATTC--- 3'
3' ---CTTAA G--- 5'

RsrII Rhodopseudomonas sphaeroides 5' CGGWCCG
3' GCCWGGC 5' ---CG GWCCG--- 3'
3' ---GCCWG GC--- 5'

Rsr2I Rhodobacter sphaeroides SE-I2 5' CGGWCCG
3' GCCWGGC 5' ---CG GWCCG--- 3'
3' ---GCCWG GC--- 5'

RtrI Rhizobium trifolii 5' GTCGAC
3' CAGCTG 5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'

Rtr63I Rhizobium trifolii 63 5' GTCGAC
3' CAGCTG 5' ---G TCGAC--- 3'
3' ---CAGCT G--- 5'

Notes

^ Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.
^ Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.
^ Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.
^ Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.
^ Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.
^ Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.
^ Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.

[pmid4588280-1] Smith HO, Nathans D (December 1973). "Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes". J. Mol. Biol. 81 (3): 419–23. doi:10.1016/0022-2836(73)90152-6. PMID 4588280.

[pmid12654995-2] Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, Bitinaite J, Blumenthal RM, Degtyarev SK, Dryden DT, Dybvig K, Firman K, Gromova ES, Gumport RI, Halford SE, Hattman S, Heitman J, Hornby DP, Janulaitis A, Jeltsch A, Josephsen J, Kiss A, Klaenhammer TR, Kobayashi I, Kong H, Krüger DH, Lacks S, Marinus MG, Miyahara M, Morgan RD, Murray NE, Nagaraja V, Piekarowicz A, Pingoud A, Raleigh E, Rao DN, Reich N, Repin VE, Selker EU, Shaw PC, Stein DC, Stoddard BL, Szybalski W, Trautner TA, Van Etten JL, Vitor JM, Wilson GG, Xu SY (April 2003). "A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes". Nucleic Acids Res. 31 (7): 1805–12. doi:10.1093/nar/gkg274. PMC 152790. PMID 12654995.

[3] Jeremy MB, John LT, Lubert S (2002). "3. Protein Structure and Function". Biochemistry. San Francisco: W. H. Freeman. ISBN 0-7167-4684-0.

[4] Anfinsen C.B. (1973). "Principles that Govern the Folding of Protein Chains". Science. 181 (4096): 223–30. doi:10.1126/science.181.4096.223. PMID 4124164.

[pmid2172084-5] Kessler C, Manta V (August 1990). "Specificity of restriction endonucleases and DNA modification methyltransferases a review (Edition 3)". Gene. 92 (1–2): 1–248. doi:10.1016/0378-1119(90)90486-B. PMID 2172084.

[pmid11557805-6] Pingoud A, Jeltsch A (September 2001). "Structure and function of type II restriction endonucleases". Nucleic Acids Res. 29 (18): 3705–27. doi:10.1093/nar/29.18.3705. PMC 55916. PMID 11557805.

[isbn0-7167-4366-3-7] Monty Krieger; Matthew P Scott; Matsudaira, Paul T.; Lodish, Harvey F.; Darnell, James E.; Lawrence Zipursky; Kaiser, Chris; Arnold Berk (2004). Molecular Cell Biology (5th ed.). New York: W.H. Freeman and Company. ISBN 0-7167-4366-3.

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