MAPRE1

Microtubule-associated protein RP/EB family member 1 is a protein that in humans is encoded by the MAPRE1 gene.

MAPRE1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1PA7, 1TXQ, 1UEG, 1VKA, 1WU9, 1YIB, 1YIG, 2HKQ, 2HL3, 2HL5, 2QJZ, 2R8U, 3GJO, 3MTU, 3TQ7, 4XA1, 4XA3, 4XA6
Identifiers
Aliases	MAPRE1, EB1, microtubule associated protein RP/EB family member 1
External IDs	OMIM: 603108; MGI: 891995; HomoloGene: 56129; GeneCards: MAPRE1; OMA:MAPRE1 - orthologs
Gene location (Human) Chr. Chromosome 20 (human) Band 20q11.21 Start 32,819,954 bp End 32,850,405 bp
Gene location (Mouse) Chr. Chromosome 2 (mouse) Band 2 H1|2 75.95 cM Start 153,583,194 bp End 153,615,230 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in secondary oocyte ganglionic eminence ventricular zone islet of Langerhans cartilage tissue mononuclear cell monocyte bone marrow tail of epididymis mucosa of sigmoid colon Top expressed in medial ganglionic eminence human fetus retinal pigment epithelium dermis atrioventricular valve maxillary prominence atrium epithelium of lens mandibular prominence endocardial cushion More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein binding microtubule binding protein C-terminus binding microtubule plus-end binding identical protein binding RNA binding cadherin binding protein kinase binding Cellular component microtubule plus-end microtubule cytoskeleton microtubule organizing center cortical microtubule cytoskeleton cell projection membrane Golgi apparatus centrosome cytoskeleton cell projection spindle cytoplasmic microtubule cytosol microtubule cytoplasm focal adhesion spindle midzone mitotic spindle astral microtubule end Biological process G2/M transition of mitotic cell cycle positive regulation of microtubule plus-end binding cell population proliferation negative regulation of microtubule polymerization cell division cell cycle protein localization to microtubule sister chromatid cohesion negative regulation of microtubule binding positive regulation of cell migration ciliary basal body-plasma membrane docking regulation of microtubule cytoskeleton organization regulation of G2/M transition of mitotic cell cycle protein localization cell migration regulation of microtubule polymerization or depolymerization positive regulation of microtubule polymerization spindle assembly protein localization to microtubule plus-end Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 22919 13589 Ensembl ENSG00000101367 ENSMUSG00000027479 UniProt Q15691 Q61166 RefSeq (mRNA) NM_012325 NM_007896 RefSeq (protein) NP_036457 NP_031922 Location (UCSC) Chr 20: 32.82 – 32.85 Mb Chr 2: 153.58 – 153.62 Mb PubMed search
Wikidata
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Function

The protein encoded by this gene was first identified by its binding to the APC (Adenomatous polyposis coli) protein which is often mutated in familial and sporadic forms of colorectal cancer.

Immunofluorescence has demonstrated that EB1 localizes to the centrosome, mitotic spindle, and distal tips of cytoplasmic microtubules. Throughout the cell cycle, EB1 proteins situate on the microtubule plus ends, which is why EB1 is categorized as a microtubule plus end tracking protein(+TIP protein).

The protein also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that this protein is involved in the regulation of microtubule structures and chromosome stability. This gene is a member of the RP/EB family.

Interactions

MAPRE1 has been shown to interact with TERF1.

Further reading

• Tirnauer JS, Bierer BE (2000). "EB1 proteins regulate microtubule dynamics, cell polarity, and chromosome stability". J. Cell Biol. 149 (4): 761–6. doi:10.1083/jcb.149.4.761. PMC 2174556. PMID 10811817.
• Morrison EE, Wardleworth BN, Askham JM, Markham AF, Meredith DM (1999). "EB1, a protein which interacts with the APC tumour suppressor, is associated with the microtubule cytoskeleton throughout the cell cycle". Oncogene. 17 (26): 3471–7. doi:10.1038/sj.onc.1202247. PMID 10030671.
• Juwana JP, Henderikx P, Mischo A, Wadle A, Fadle N, Gerlach K, Arends JW, Hoogenboom H, Pfreundschuh M, Renner C (1999). "EB/RP gene family encodes tubulin binding proteins". Int. J. Cancer. 81 (2): 275–84. doi:10.1002/(SICI)1097-0215(19990412)81:2<275::AID-IJC18>3.0.CO;2-Z. PMID 10188731. S2CID 33232892.
• Berrueta L, Tirnauer JS, Schuyler SC, Pellman D, Bierer BE (1999). "The APC-associated protein EB1 associates with components of the dynactin complex and cytoplasmic dynein intermediate chain". Curr. Biol. 9 (8): 425–8. Bibcode:1999CBio....9..425B. doi:10.1016/S0960-9822(99)80190-0. PMID 10226031. S2CID 14191776.
• Nakagawa H, Koyama K, Murata Y, Morito M, Akiyama T, Nakamura Y (2000). "EB3, a novel member of the EB1 family preferentially expressed in the central nervous system, binds to a CNS-specific APC homologue". Oncogene. 19 (2): 210–6. doi:10.1038/sj.onc.1203308. PMID 10644998.
• Askham JM, Moncur P, Markham AF, Morrison EE (2000). "Regulation and function of the interaction between the APC tumour suppressor protein and EB1". Oncogene. 19 (15): 1950–8. doi:10.1038/sj.onc.1203498. PMID 10773885.
• Mimori-Kiyosue Y, Shiina N, Tsukita S (2000). "The dynamic behavior of the APC-binding protein EB1 on the distal ends of microtubules". Curr. Biol. 10 (14): 865–8. Bibcode:2000CBio...10..865M. doi:10.1016/S0960-9822(00)00600-X. PMID 10899006. S2CID 18926885.
• Zhou XZ, Lu KP (2001). "The Pin2/TRF1-interacting protein PinX1 is a potent telomerase inhibitor". Cell. 107 (3): 347–59. CiteSeerX 10.1.1.326.1815. doi:10.1016/S0092-8674(01)00538-4. PMID 11701125. S2CID 6822193.
• Nakamura M, Zhou XZ, Kishi S, Lu KP (2002). "Involvement of the telomeric protein Pin2/TRF1 in the regulation of the mitotic spindle". FEBS Lett. 514 (2–3): 193–8. doi:10.1016/S0014-5793(02)02363-3. PMID 11943150. S2CID 2579290.
• Askham JM, Vaughan KT, Goodson HV, Morrison EE (2003). "Evidence that an interaction between EB1 and p150(Glued) is required for the formation and maintenance of a radial microtubule array anchored at the centrosome". Mol. Biol. Cell. 13 (10): 3627–45. doi:10.1091/mbc.E02-01-0061. PMC 129971. PMID 12388762.
• Tirnauer JS, Canman JC, Salmon ED, Mitchison TJ (2003). "EB1 targets to kinetochores with attached, polymerizing microtubules". Mol. Biol. Cell. 13 (12): 4308–16. doi:10.1091/mbc.E02-04-0236. PMC 138635. PMID 12475954.
• Ligon LA, Shelly SS, Tokito M, Holzbaur EL (2003). "The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization". Mol. Biol. Cell. 14 (4): 1405–17. doi:10.1091/mbc.E02-03-0155. PMC 153110. PMID 12686597.
• Hayashi I, Ikura M (2003). "Crystal structure of the amino-terminal microtubule-binding domain of end-binding protein 1 (EB1)". J. Biol. Chem. 278 (38): 36430–4. doi:10.1074/jbc.M305773200. PMID 12857735.
• Bu W, Su LK (2004). "Characterization of functional domains of human EB1 family proteins". J. Biol. Chem. 278 (50): 49721–31. doi:10.1074/jbc.M306194200. PMID 14514668.
• Bieling P, Laan L, Schek H, Munteanu EL, Sandblad L, Dogterom M, Brunner D, Surrey T (2007). "Reconstitution of a microtubule plus-end tracking system in vitro". Nature. 450 (7172): 1100–5. Bibcode:2007Natur.450.1100B. doi:10.1038/nature06386. PMID 18059460. S2CID 4355669.
• Maurer SP, Fourniol FJ, Bohner G, Moores CA, Surrey T (2012). "EBs recognize a nucleotide-dependent structural cap at growing microtubule ends". Cell. 149 (2): 371–82. doi:10.1016/j.cell.2012.02.049. PMC 3368265. PMID 22500803.
• Nakamura S, Grigoriev I, Nogi T, Hamaji T, Cassimeris L, Mimori-Kiyosue Y (2012). "Dissecting the nanoscale distributions and functions of microtubule-end-binding proteins EB1 and ch-TOG in interphase HeLa cells". PLOS ONE. 7 (12): e51442. Bibcode:2012PLoSO...751442N. doi:10.1371/journal.pone.0051442. PMC 3520847. PMID 23251535.
• Zanic M, Widlund PO, Hyman AA, Howard J (2013). "Synergy between XMAP215 and EB1 increases microtubule growth rates to physiological levels". Nat. Cell Biol. 15 (6): 688–93. doi:10.1038/ncb2744. PMID 23666085. S2CID 3025200.
• Jie, Chen (2014). "Phosphoregulation of the dimerization and functions of end-binding protein 1". Protein & Cell. 5 (10): 795–799. doi:10.1007/s13238-014-0081-9. PMC 4180461. PMID 25048701.