ME1 (gene)

NADP-dependent malic enzyme is a protein that in humans is encoded by the ME1 gene.

ME1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2AW5, 3WJA
Identifiers
Aliases	ME1, HUMNDME, MES, malic enzyme 1
External IDs	OMIM: 154250; MGI: 97043; HomoloGene: 134785; GeneCards: ME1; OMA:ME1 - orthologs
EC number	1.1.1.40
Gene location (Human) Chr. Chromosome 6 (human) Band 6q14.2 Start 83,210,402 bp End 83,431,051 bp
Gene location (Mouse) Chr. Chromosome 9 (mouse) Band 9 E3.1|9 46.58 cM Start 86,463,424 bp End 86,578,006 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in Skeletal muscle tissue of biceps brachii spinal ganglia trigeminal ganglion pars compacta skin of thigh pars reticulata right adrenal cortex vulva gums gingival epithelium Top expressed in adrenal gland lactiferous gland tunica adventitia of aorta mucous cell of stomach pyloric antrum right kidney epithelium of stomach subcutaneous adipose tissue brown adipose tissue zygote More reference expression data BioGPS More reference expression data
Gene ontology Molecular function manganese ion binding ADP binding NAD binding metal ion binding NADP binding electron transfer activity malate dehydrogenase (decarboxylating) (NAD+) activity oxidoreductase activity oxaloacetate decarboxylase activity malic enzyme activity malate dehydrogenase (decarboxylating) (NADP+) activity Cellular component cytoplasm cytosol mitochondrion Biological process regulation of NADP metabolic process protein tetramerization NADP biosynthetic process response to hormone response to carbohydrate malate metabolic process regulation of lipid metabolic process carbohydrate metabolic process electron transport chain pyruvate metabolic process nucleotide biosynthetic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4199 17436 Ensembl ENSG00000065833 ENSMUSG00000032418 UniProt P48163 P06801 RefSeq (mRNA) NM_002395 NM_001198933 NM_008615 RefSeq (protein) NP_002386 NP_001185862 NP_032641 Location (UCSC) Chr 6: 83.21 – 83.43 Mb Chr 9: 86.46 – 86.58 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

This gene encodes a cytosolic, NADP-dependent enzyme that generates NADPH for fatty acid biosynthesis. The activity of this enzyme, the reversible oxidative decarboxylation of malate to pyruvate, links the glycolytic and citric acid cycles. The regulation of expression for this gene is complex. Increased expression can result from elevated levels of thyroid hormones or by higher proportions of carbohydrates in the diet.

Further reading

• Povey S, Wilson DE, Harris H, et al. (1978). "Sub-unit structure of soluble and mitochondrial malic enzyme: demonstration of human mitochondrial enzyme in human-mouse hybrids". Ann. Hum. Genet. 39 (2): 203–12. doi:10.1111/j.1469-1809.1975.tb00123.x. PMID 1088824. S2CID 39275324.
• Tessarolo D, Liguori M, Giacanelli M (1991). "Human malic enzymes in heart and muscle: evidence of a selective distribution". Biochem. Med. Metab. Biol. 45 (1): 1–5. doi:10.1016/0885-4505(91)90002-3. PMID 2015102.
• Taroni F, Di Donato S (1988). "Purification and properties of cytosolic malic enzyme from human skeletal muscle". Int. J. Biochem. 20 (8): 857–66. doi:10.1016/0020-711X(88)90075-4. PMID 3169368.
• Chen TR, McMorris FA, Creagan R, et al. (1973). "Assignment of the genes for malate oxidoreductase decarboxylating to chromosome 6 and peptidase B and lactate dehydrogenase B to chromosome 12 in man". Am. J. Hum. Genet. 25 (2): 200–7. PMC 1762508. PMID 4689040.
• Bukato G, Kochan Z, Swierczyński J (1996). "Different regulatory properties of the cytosolic and mitochondrial forms of malic enzyme isolated from human brain". Int. J. Biochem. Cell Biol. 27 (10): 1003–8. doi:10.1016/1357-2725(95)00080-9. PMID 7496989.
• González-Manchón C, Ferrer M, Ayuso MS, Parrilla R (1995). "Cloning, sequencing and functional expression of a cDNA encoding a NADP-dependent malic enzyme from human liver". Gene. 159 (2): 255–60. doi:10.1016/0378-1119(95)00004-P. PMID 7622060.
• Bukato G, Kochan Z, Swierczyński J (1995). "Purification and properties of cytosolic and mitochondrial malic enzyme isolated from human brain". Int. J. Biochem. Cell Biol. 27 (1): 47–54. doi:10.1016/1357-2725(94)00057-3. PMID 7757881.
• Maruyama K, Sugano S (1994). "Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides". Gene. 138 (1–2): 171–4. doi:10.1016/0378-1119(94)90802-8. PMID 8125298.
• Chou WY, Huang SM, Chang GG (1996). "Nonidentity of the cDNA sequence of human breast cancer cell malic enzyme to that from the normal human cell". J. Protein Chem. 15 (3): 273–9. doi:10.1007/BF01887116. PMID 8804575. S2CID 31679272.
• González-Manchón C, Butta N, Ferrer M, et al. (1997). "Molecular cloning and functional characterization of the human cytosolic malic enzyme promoter: thyroid hormone responsiveness". DNA Cell Biol. 16 (5): 533–44. doi:10.1089/dna.1997.16.533. PMID 9174159.
• Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
• Sylvius N, Tesson F, Gayet C, et al. (2001). "A new locus for autosomal dominant dilated cardiomyopathy identified on chromosome 6q12-q16". Am. J. Hum. Genet. 68 (1): 241–6. doi:10.1086/316929. PMC 1234920. PMID 11085912.
• Butta N, González-Manchón C, Arias-Salgado EG, et al. (2001). "Cloning and functional characterization of the 5' flanking region of the human mitochondrial malic enzyme gene. Regulatory role of Sp1 and AP-2". Eur. J. Biochem. 268 (10): 3017–27. doi:10.1046/j.1432-1327.2001.02194.x. hdl:10261/73615. PMID 11358520.
• Yang Z, Lanks CW, Tong L (2002). "Molecular mechanism for the regulation of human mitochondrial NAD(P)+-dependent malic enzyme by ATP and fumarate". Structure. 10 (7): 951–60. doi:10.1016/S0969-2126(02)00788-8. PMID 12121650.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Mungall AJ, Palmer SA, Sims SK, et al. (2003). "The DNA sequence and analysis of human chromosome 6". Nature. 425 (6960): 805–11. Bibcode:2003Natur.425..805M. doi:10.1038/nature02055. PMID 14574404.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Hsieh JY, Liu GY, Chang GG, Hung HC (2006). "Determinants of the dual cofactor specificity and substrate cooperativity of the human mitochondrial NAD(P)+-dependent malic enzyme: functional roles of glutamine 362". J. Biol. Chem. 281 (32): 23237–45. doi:10.1074/jbc.M603451200. PMID 16757477.