Mediator of RNA polymerase II transcription subunit 8 is an enzyme that in humans is encoded by the MED8 gene .[ 5] [ 6] [ 7]
This gene encodes a protein that is one of more than 20 subunits of the mediator complex, first identified in S. cerevisiae, that is required for activation of transcription. The product of this gene also interacts with elongins B and C, and CUL2 and RBX1, to reconstitute a ubiquitin ligase. Five alternative transcripts encoding four isoforms have been described.[ 7]
^ a b c GRCh38: Ensembl release 89: ENSG00000159479 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000006392 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Brower CS, Sato S, Tomomori-Sato C, Kamura T, Pause A, Stearman R, Klausner RD, Malik S, Lane WS, Sorokina I, Roeder RG, Conaway JW, Conaway RC (Aug 2002). "Mammalian mediator subunit mMED8 is an Elongin BC-interacting protein that can assemble with Cul2 and Rbx1 to reconstitute a ubiquitin ligase" . Proc Natl Acad Sci U S A . 99 (16): 10353–8. Bibcode :2002PNAS...9910353B . doi :10.1073/pnas.162424199 PMC 124918 PMID 12149480 . ^ Jiang YW, Veschambre P, Erdjument-Bromage H, Tempst P, Conaway JW, Conaway RC, Kornberg RD (Aug 1998). "Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways" . Proc Natl Acad Sci U S A . 95 (15): 8538–43. Bibcode :1998PNAS...95.8538J . doi :10.1073/pnas.95.15.8538 PMC 21111 PMID 9671713 . ^ a b "Entrez Gene: MED8 mediator of RNA polymerase II transcription, subunit 8 homolog (S. cerevisiae)" .^ Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (Jun 2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology" . Mol. Cell . 14 (5): 685–91. doi :10.1016/j.molcel.2004.05.006 PMID 15175163 .
Näär AM, Beaurang PA, Zhou S, Abraham S, Solomon W, Tjian R (1999). "Composite co-activator ARC mediates chromatin-directed transcriptional activation". Nature . 398 (6730): 828–32. Bibcode :1999Natur.398..828N . doi :10.1038/19789 . PMID 10235267 . S2CID 23646963 . Xu XR, Huang J, Xu ZG, Qian BZ, Zhu ZD, Yan Q, Cai T, Zhang X, Xiao HS, Qu J, Liu F, Huang QH, Cheng ZH, Li NG, Du JJ, Hu W, Shen KT, Lu G, Fu G, Zhong M, Xu SH, Gu WY, Huang W, Zhao XT, Hu GX, Gu JR, Chen Z, Han ZG (2002). "Insight into hepatocellular carcinogenesis at transcriptome level by comparing gene expression profiles of hepatocellular carcinoma with those of corresponding noncancerous liver" . Proc. Natl. Acad. Sci. U.S.A . 98 (26): 15089–94. Bibcode :2001PNAS...9815089X . doi :10.1073/pnas.241522398 PMC 64988 PMID 11752456 . Sato S, Tomomori-Sato C, Banks CA, Sorokina I, Parmely TJ, Kong SE, Jin J, Cai Y, Lane WS, Brower CS, Conaway RC, Conaway JW (2003). "Identification of mammalian Mediator subunits with similarities to yeast Mediator subunits Srb5, Srb6, Med11, and Rox3" . J. Biol. Chem . 278 (17): 15123–7. doi :10.1074/jbc.C300054200 PMID 12584197 . Sato S, Tomomori-Sato C, Banks CA, Parmely TJ, Sorokina I, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian homolog of Drosophila melanogaster transcriptional coactivator intersex is a subunit of the mammalian Mediator complex" . J. Biol. Chem . 278 (50): 49671–4. doi :10.1074/jbc.C300444200 PMID 14576168 . Tomomori-Sato C, Sato S, Parmely TJ, Banks CA, Sorokina I, Florens L, Zybailov B, Washburn MP, Brower CS, Conaway RC, Conaway JW (2004). "A mammalian mediator subunit that shares properties with Saccharomyces cerevisiae mediator subunit Cse2" . J. Biol. Chem . 279 (7): 5846–51. doi :10.1074/jbc.M312523200 PMID 14638676 . Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC (2004). "A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology" . Mol. Cell . 14 (5): 685–91. doi :10.1016/j.molcel.2004.05.006 PMID 15175163 . Léonard VH, Kohl A, Hart TJ, Elliott RM (2006). "Interaction of Bunyamwera Orthobunyavirus NSs Protein with Mediator Protein MED8: a Mechanism for Inhibiting the Interferon Response" . J. Virol . 80 (19): 9667–75. doi :10.1128/JVI.00822-06 . PMC 1617248 PMID 16973571 .
