MGAT1

Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase is an enzyme that in humans is encoded by the MGAT1 gene.

MGAT1
Identifiers
Aliases	MGAT1, GLCNAC-TI, GLCT1, GLYT1, GNT-1, GNT-I, MGAT, GnTI, mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase
External IDs	OMIM: 160995; MGI: 96973; HomoloGene: 1804; GeneCards: MGAT1; OMA:MGAT1 - orthologs
Gene location (Human) Chr. Chromosome 5 (human) Band 5q35.3 Start 180,784,782 bp End 180,815,652 bp
Gene location (Mouse) Chr. Chromosome 11 (mouse) Band 11 B1.2|11 29.27 cM Start 49,135,018 bp End 49,153,857 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in monocyte granulocyte right lung gastric mucosa upper lobe of left lung apex of heart right lobe of thyroid gland left uterine tube spleen left lobe of thyroid gland Top expressed in neural layer of retina granulocyte thymus yolk sac right kidney choroid plexus of fourth ventricle proximal tubule tibiofemoral joint molar lip More reference expression data BioGPS More reference expression data
Gene ontology Molecular function glycosyltransferase activity transferase activity acetylglucosaminyltransferase activity metal ion binding alpha-1,3-mannosylglycoprotein 2-beta-N-acetylglucosaminyltransferase activity manganese ion binding Cellular component integral component of membrane extracellular vesicle Golgi membrane Golgi apparatus extracellular exosome membrane Biological process protein glycosylation in utero embryonic development UDP-N-acetylglucosamine catabolic process carbohydrate metabolic process protein N-linked glycosylation protein N-linked glycosylation via asparagine Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 4245 17308 Ensembl ENSG00000131446 ENSMUSG00000020346 UniProt P26572 P27808 RefSeq (mRNA) NM_001114617 NM_001114618 NM_001114619 NM_001114620 NM_002406 NM_001110148 NM_001110149 NM_001110150 NM_010794 RefSeq (protein) NP_001108089 NP_001108090 NP_001108091 NP_001108092 NP_002397 NP_001351306 NP_001351308 NP_001351309 NP_001351310 NP_001351311 NP_001351312 NP_001351313 NP_001351314 NP_001351315 NP_001351316 NP_001351317 NP_001351318 NP_001351319 NP_001351320 NP_001351321 NP_001351322 NP_001351323 NP_001351324 NP_001103618 NP_001103619 NP_001103620 NP_034924 Location (UCSC) Chr 5: 180.78 – 180.82 Mb Chr 11: 49.14 – 49.15 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

There are over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. UDP-N-acetylglucosamine:alpha-3-D-mannoside beta-1,2-N-acetylglucosaminyltransferase I is a medial-Golgi enzyme essential for the synthesis of hybrid and complex N-glycans. The protein, encoded by a single exon, shows typical features of a type II transmembrane protein. The protein is believed to be essential for normal embryogenesis.

Further reading

• Kumar R, Yang J, Eddy RL, et al. (1992). "Cloning and expression of the murine gene and chromosomal location of the human gene encoding N-acetylglucosaminyltransferase I". Glycobiology. 2 (4): 383–93. doi:10.1093/glycob/2.4.383. PMID 1421759.
• Kumar R, Yang J, Larsen RD, Stanley P (1991). "Cloning and expression of N-acetylglucosaminyltransferase I, the medial Golgi transferase that initiates complex N-linked carbohydrate formation". Proc. Natl. Acad. Sci. U.S.A. 87 (24): 9948–52. doi:10.1073/pnas.87.24.9948. PMC 55291. PMID 1702225.
• Tan J, D'Agostaro AF, Bendiak B, et al. (1995). "The human UDP-N-acetylglucosamine: alpha-6-D-mannoside-beta-1,2- N-acetylglucosaminyltransferase II gene (MGAT2). Cloning of genomic DNA, localization to chromosome 14q21, expression in insect cells and purification of the recombinant protein". Eur. J. Biochem. 231 (2): 317–28. doi:10.1111/j.1432-1033.1995.tb20703.x. PMID 7635144.
• Yip B, Chen SH, Mulder H, et al. (1997). "Organization of the human beta-1,2-N-acetylglucosaminyltransferase I gene (MGAT1), which controls complex and hybrid N-glycan synthesis". Biochem. J. 321 (2): 465–74. doi:10.1042/bj3210465. PMC 1218092. PMID 9020882.
• Yen CL, Stone SJ, Cases S, et al. (2002). "Identification of a gene encoding MGAT1, a monoacylglycerol acyltransferase". Proc. Natl. Acad. Sci. U.S.A. 99 (13): 8512–7. Bibcode:2002PNAS...99.8512Y. doi:10.1073/pnas.132274899. PMC 124292. PMID 12077311.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039.
• Colland F, Jacq X, Trouplin V, et al. (2004). "Functional proteomics mapping of a human signaling pathway". Genome Res. 14 (7): 1324–32. doi:10.1101/gr.2334104. PMC 442148. PMID 15231748.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
• Otsuki T, Ota T, Nishikawa T, et al. (2007). "Signal sequence and keyword trap in silico for selection of full-length human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA libraries". DNA Res. 12 (2): 117–26. doi:10.1093/dnares/12.2.117. PMID 16303743.
• Saribas AS, Johnson K, Liu L, et al. (2007). "Refolding of human beta-1-2 GlcNAc transferase (GnT1) and the role of its unpaired Cys 121". Biochem. Biophys. Res. Commun. 362 (2): 381–6. doi:10.1016/j.bbrc.2007.07.199. PMID 17716624.