In enzymology , a mRNA (guanine-N7-)-methyltransferase also known as mRNA cap guanine-N7 methyltransferase is an enzyme that catalyzes the chemical reaction
RNMT Identifiers Aliases RNMT External IDs OMIM : 603514 ; MGI : 1915147 ; HomoloGene : 2816 ; GeneCards : RNMT ; OMA :RNMT - orthologs Wikidata
S -adenosyl-L -methionine + G(5')pppR-RNA
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{\displaystyle \rightleftharpoons }
S -adenosyl-L -homocysteine + m7 G(5')pppR-RNA (mRNA containing an N7 -methylguanine cap)Thus, the two substrates of this enzyme are S-adenosyl methionine and G(5')pppR-RNA , whereas its two products are S-adenosylhomocysteine and m7G(5')pppR-RNA . This enzyme belongs to the family of transferases , specifically those transferring one-carbon group methyltransferases.
In humans, mRNA cap guanine-N7 methyltransferase is encoded by the RNMT gene .[ 5] [ 6] [ 7]
The systematic name of this enzyme class is S-adenosyl-L-methionine:mRNA (guanine-N7-)-methyltransferase . Other names in common use include:
messenger ribonucleate guanine 7-methyltransferase,
guanine-7-methyltransferase,
messenger RNA guanine 7-methyltransferase, and
S-adenosyl-L-methionine:mRNA (guanine-7-N-)-methyltransferase.
cap MTase
^ a b c GRCh38: Ensembl release 89: ENSG00000101654 – Ensembl , May 2017^ a b c GRCm38: Ensembl release 89: ENSMUSG00000009535 – Ensembl , May 2017^ "Human PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ "Mouse PubMed Reference:" . National Center for Biotechnology Information, U.S. National Library of Medicine .^ Pillutla RC, Shimamoto A, Furuichi Y, Shatkin AJ (Jan 1999). "Human mRNA capping enzyme (RNGTT) and cap methyltransferase (RNMT) map to 6q16 and 18p11.22-p11.23, respectively". Genomics . 54 (2): 351–3. doi :10.1006/geno.1998.5604 . PMID 9828141 . ^ Pillutla RC, Yue Z, Maldonado E, Shatkin AJ (Sep 1998). "Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes" . J Biol Chem . 273 (34): 21443–6. doi :10.1074/jbc.273.34.21443 PMID 9705270 . ^ "Entrez Gene: RNMT RNA (guanine-7-) methyltransferase" .
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