M line may refer to:
- M-line (mittel line or middle line), a structure in a muscle sarcomere
- M Ocean View, a light rail and former streetcar line in San Francisco, California
- McKinney Avenue Transit Authority, a streetcar line in Dallas, Texas also named the M-Line
- M (Los Angeles Railway), a former streetcar service
- Geometric mean
See also
edit- M Train (disambiguation), several train services
- M1 (disambiguation)#Railways
- Line M (disambiguation)