Macrobiotus shonaicus (Japanese: ショウナイチョウメイムシ) is a species of tardigrade in the family Macrobiotidae. As of 2018[update] it is only known from its type locality: Tsuruoka, Japan. The species description was published in 2018. The insides of their first three pairs of legs have a slight fold above their claws, and their eggs have processes whose terminal discs split off into thin filaments.
| Macrobiotus shonaicus | |
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| A. Dorsoventral view of holotype (PCM); B. Dorsal view of paratype (SEM); C–E. Close up of anterior, median, and posterior, respectively, of paratype, showing pores (SEM). Scale bars in μm. | |
Scientific classification 
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| Domain: | Eukaryota |
| Kingdom: | Animalia |
| Phylum: | Tardigrada |
| Class: | Eutardigrada |
| Order: | Parachela |
| Family: | Macrobiotidae |
| Genus: | Macrobiotus |
| Species: | M. shonaicus
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| Binomial name | |
| Macrobiotus shonaicus Stec et al., 2018
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| Type locality of M. shonaicus | |
Taxonomic history
editThe species description was written by Daniel Stec, Kazuharu Arakawa, and Łukasz Michalczyk; it was published in PLOS One in February 2018.[1]
Arakawa collected ten M. shonaicus specimens in May 2016 from moss growing in his apartment building's parking lot, in Tsuruoka, Japan.[1]: 2 The moss, Bryum argenteum, was growing on a concrete surface. One pair of these tardigrades successfully reproduced in vitro, yielding an isogenic strain.[1]: 2 The holotype, 57 paratypes, and 34 eggs were deposited at Jagiellonian University in Kraków. An additional 17 paratypes and 7 eggs were deposited at Keio University, Tsuruoka.[1]: 15–16
The specific epithet, shonaicus, is named after Shōnai, which the authors describe as "the region in Japan where the new species was collected".[1]: 15
Distribution
editThe type locality is 38°44′24″N 139°48′26″E / 38.74000°N 139.80722°E, "Otsuka-machi, Tsuruoka-City, Japan".[1]: 2, 15 It is found widely throughout Japan.[2]
Description
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Juveniles have a white body, while the body of adults is a slightly yellowish. They have eyes. Round pores with a diameter of 0.2–0.4 μm are scattered over the surface of their legs, their sides, and their backs. The internal surface of their first three pairs of legs have a bulge; these pairs also have an inside fold above the claws. This species is dioecious, but there are no secondary sex characteristics. The measurements of their body lengths range 218–743 μm.[1]: 6–8
The eggs' color ranges from white to pale yellow and their shape ranges from spherical to slightly ovoid. The full diameter of the eggs range 56.4–70.8 μm. Their surface is covered with protruding inverted goblet-shaped platforms, whose terminal disc is surrounded by "teeth" which extend out into thin, fragile filaments.[1]: 8–11
Stec and colleagues performed an experiment to see if females could reproduce parthenogenetically. Reproduction was not observed when individuals were separated and not allowed to mate, suggesting they cannot.[1]: 8
Stec and colleagues sequenced four molecular markers: 18S rRNA, 28S rRNA, ITS-2, and mitochondrial COI. DNA was sequenced from four specimens. The sequences were submitted to GenBank.[1]: 3–4 Their analysis yielded a single haplotype each for the 18S rRNA and the 28S rRNA and two haplotypes each for the ITS-2 and COI.[1]: 11–14 The most conservative marker with respect to other species in the M. hufelandi complex was 18S rRNA, which differed 0.3–3.3% from the available sequences in GenBank. This was followed by 28S rRNA (5.0–9.8%), ITS-2 (11.2–29.7%), and COI (19.6–26.9%).[1]: 20–21
Phylogeny
editStec and colleagues' analysis placed M. shonaicus in a clade along with M. paulinae, M. polypiformis, M. scoticus and M. kristenseni. This clade is within the hufelandi group.[1]: 21
References
edit- ^ a b c d e f g h i j k l m Stec, Daniel; Arakawa, Kazuharu; Michalczyk, Łukasz (28 February 2018). "An integrative description of Macrobiotus shonaicus sp. nov. (Tardigrada: Macrobiotidae) from Japan with notes on its phylogenetic position within the hufelandi group". PLOS ONE. 13 (2): e0192210. Bibcode:2018PLoSO..1392210S. doi:10.1371/journal.pone.0192210. PMC 5830310. PMID 29489835.
- ^ Sugiura, Kenta; Arakawa, Kazuharu; Matsumoto, Midori (2020). "Distribution of Macrobiotus shonaicus Stec, Arakawa & Michalczyk, 2018 (Tardigrada: Eutardigrada: Macrobiotidae) in Japan". Zootaxa. 4767 (1): 56–70. doi:10.11646/zootaxa.4767.1.2. PMID 33056572. S2CID 218997597. [Sugiura, K.; Arakawa, K.; Matsumoto, M. (2020). "Erratum". Zootaxa. 4778 (3): 600. doi:10.11646/zootaxa.4778.3.11. PMID 33055818.]
Further reading
edit- Dockrill, Peter (28 February 2018). "A Totally New Species of Tardigrade Was Just Discovered in a Japanese Carpark". ScienceAlert. Retrieved 11 March 2018.
- Gregory, Tessa. "Mysterious Moss Piglet: Researchers discover new tardigrade species". PLOS Research News. No. 28 February 2018. Retrieved 11 March 2018.
- Milius, Susan (28 February 2018). "A new species of tardigrade lays eggs covered with doodads and streamers". Science News. Retrieved 11 March 2018.
- Pappas, Stephanie (28 February 2018). "An Even-Weirder-Than-Usual Tardigrade Just Turned Up in a Parking Lot". Live Science. Retrieved 11 March 2018.
- PLOS (28 February 2018). "New tardigrade species Macrobiotus shonaicus sp. nov. identified in Japan: Researchers characterize new species using microscopy, genetic analysis". ScienceDaily.
- Sugiura, Kenta; Minato, Hiroki; Suzuki, Atsushi C.; Arakawa, Kazuharu; Kunieda, Takekazu; Matsumoto, Midori (2019). "Comparison of Sexual Reproductive Behaviors in Two Species of Macrobiotidae (Tardigrada: Eutardigrada)". Zoological Science. 36 (2): 120–127. doi:10.2108/zs180103. PMID 31120646. S2CID 145880253.
External links
edit- Arakawa, Kazuharu (8 February 2018). Macrobiotus shonaicus – ショウナイチョウメイムシ – 1 (YouTube video).
- Arakawa, Kazuharu (8 February 2018). Macrobiotus shonaicus – ショウナイチョウメイムシ – 2 (YouTube video).
- Arakawa, Kazuharu (8 February 2018). Macrobiotus shonaicus – ショウナイチョウメイムシ – 3 (YouTube video).
- Arakawa, Kazuharu (8 February 2018). Macrobiotus shonaicus – ショウナイチョウメイムシ – 4 (YouTube video).