Magdalene or Magdalen may refer to:
- Mary Magdalene, a disciple of Jesus
- Magdalene (given name), a feminine given name (including a list of people with the name)
- Magdalen College, Oxford, a constituent college of the University of Oxford
- Magdalene College, Cambridge, a constituent college of the University of Cambridge
- Magdalen Islands, an archipelago in Quebec, Canada
- Magdalene asylum or Magdalene Laundries, Catholic institutions for “fallen women”
- Magdalene (comics), a Marvel Comics character
- Magdalene (sculpture), an outdoor sculpture by Dessa Kirk in Chicago, Illinois, US
- Magdallan, later known as Magdalen, an American metal band
- Magdalene (album), a 2019 album by FKA Twigs
- "Magdalene", a song by Lenny Kravitz from Circus
- "Magdalene", a song by White Zombie from Let Sleeping Corpses Lie
- Magdalene Catholic High School, in Smeaton Grange, New South Wales, Australia
- Wiggenhall St Mary Magdalen, Norfolk