Mannosyl-oligosaccharide 1,3-1,6-α-mannosidase, also known as Golgi α-mannosidase II, is an enzyme with systematic name (1→3)-(1→6)-mannosyl-oligosaccharide α-D-mannohydrolase.[1][2][3][4] It catalyses the hydrolysis of the terminal (1→3)- and (1→6)-linked α-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3.
| Mannosyl-oligosaccharide 1,3-1,6-α-mannosidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.2.1.114 | ||||||||
| CAS no. | 82047-77-6 | ||||||||
| Alt. names | mannosidase II, exo-1,3-1,6-α-mannosidase, α-D-mannosidase II, α-mannosidase II, α-3,6-mannosidase, GlcNAc transferase I-dependent α1,3[α1,6]mannosidase, Golgi α-mannosidase II, ManII, 1,3(1,6)-α-D-mannosidase, 1,3-(1,6-)mannosyl-oligosaccharide α-D-mannohydrolase | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
This enzyme is involved in the synthesis of glycoproteins. It is a key enzyme of N-linked glycan processing and is inhibited by small molecule swainsonine.[5]
References
edit- ^ Harpaz N, Schachter H (May 1980). "Control of glycoprotein synthesis. Processing of asparagine-linked oligosaccharides by one or more rat liver Golgi α-D-mannosidases dependent on the prior action of UDP-N-acetylglucosamine: α-D-mannoside β 2-N-acetylglucosaminyltransferase I". The Journal of Biological Chemistry. 255 (10): 4894–902. PMID 6445359.
- ^ Tulsiani DR, Hubbard SC, Robbins PW, Touster O (Apr 1982). "α-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes converting Man9 precursors to Man5 intermediates". The Journal of Biological Chemistry. 257 (7): 3660–8. PMID 7061502.
- ^ Tulsiani DR, Opheim DJ, Touster O (May 1977). "Purification and characterization of α-D-mannosidase from rat liver Golgi membranes". The Journal of Biological Chemistry. 252 (10): 3227–33. PMID 863880.
- ^ Athanasopoulos VI, Niranjan K, Rastall RA (Mar 2005). "The production, purification and characterisation of two novel α-D-mannosidases from Aspergillus phoenicis". Carbohydrate Research. 340 (4): 609–17. doi:10.1016/j.carres.2005.01.005. PMID 15721331.
- ^ van den Elsen JM, Kuntz DA, Rose DR (2001). "Structure of Golgi α-mannosidase II: a target for inhibition of growth and metastasis of cancer cells". The EMBO Journal. 20 (12): 3008–3017. doi:10.1093/emboj/20.12.3008. PMC 150216. PMID 11406577.
External links
edit- Mannosyl-oligosaccharide+1,3-1,6-α-mannosidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
