This is the progression of world record improvements of the 400 metres W40 division of Masters athletics.
- Key
Incomplete information Pending
| Hand | Auto | Athlete | Nationality | Birthdate | Location | Date |
|---|---|---|---|---|---|---|
| 52.50 | Geisa Aparecida Coutinho |  Brazil
|
1 June 1980 | Bragança Paulista | 9 April 2021 | |
| 53.05 A | Maria Magnolia Figueiredo | Brazil
|
11 November 1963 | Bogotà | 11 July 2004 | |
| 53.68 A | Sara Montecinos |  Chile
|
8 March 1954 | Cali | 20 March 1994 | |
| 55.38 | Maeve Kyle |  Ireland
|
6 October 1928 | Edinburgh | 22 July 1970 |