Methylocella silvestris is a bacterium from the genus Methylocella spp which are found in many acidic soils and wetlands.[1] Historically, Methylocella silvestris was originally isolated from acidic forest soils in Germany, and it is described as Gram-negative, aerobic, non-pigmented, non-motile, rod-shaped and methane-oxidizing facultative methanotroph.[2] As an aerobic methanotrophic bacteria, Methylocella spp use methane (CH4), and methanol as their main carbon and energy source, as well as multi compounds acetate, pyruvate, succinate, malate, and ethanol.[3] They were known to survive in the cold temperature from 4° to 30° degree of Celsius with the optimum at around 15° to 25 °C, but no more than 36 °C. They grow better in the pH scale between 4.5 to 7.0.[1] It lacks intracytoplasmic membranes common to all methane-oxidizing bacteria except Methylocella, but contain a vesicular membrane system connected to the cytoplasmic membrane. BL2T (=DSM 15510T=NCIMB 13906T) is the type strain.
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| Species: | M. silvestris
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| Methylocella silvestris Dunfield et al., 2003
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Phylogenetic
editDunfield et.al mentioned that Methylocella silvestris is close related with Methylocella palustris KT, Beijerinckia indica ATCC 9039, and Methylocapsa acipihila B2T in terms of its phylogenetic, which make M. silvestris classified as a type II methanotroph that utilize the serine cycle for their carbon assimilation, but it does not have a soluble methane monooxygenase (sMMO) and a propane monooxygenase (PrMO).[1][4]
Genome
editThe genome of Methylocella silvestris is sequenced. Methylocella silvestris contains eight genes which can encode NAD(P)-dependent alcohol dehydrogenases (ADHs), pyrroloquinoline quinone (PQQ)-containing methanol dehydrogenase (Msil_0471) and a PQQ-containing ADH with 73% identity to xoxF from Methylobacterium extorquens (Msil_1587).[4] Chen et.al stated in their article that the genome size is 4.3 MbP, and has similarity to Proteobacteria.[2]
References
edit- ^ a b c Dunfield PF, Khmelenina VN, Suzina NE, Trotsenko YA, Dedysh SN (September 2003). "Methylocella silvestris sp. nov., a novel methanotroph isolated from an acidic forest cambisol". International Journal of Systematic and Evolutionary Microbiology. 53 (Pt 5): 1231–1239. doi:10.1099/ijs.0.02481-0. PMID 13130000. Retrieved 2013-07-25.
- ^ a b Chen Y, Crombie A, Rahman MT, Dedysh SN, Liesack W, Stott MB, et al. (July 2010). "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2". Journal of Bacteriology. 192 (14): 3840–1. doi:10.1128/JB.00506-10. PMC 2897342. PMID 20472789.
- ^ Dedysh, Svetlana N.; Knief, Claudia; Dunfield, Peter F. (2005-07-01). "Methylocella Species Are Facultatively Methanotrophic". Journal of Bacteriology. 187 (13): 4665–4670. doi:10.1128/JB.187.13.4665-4670.2005. ISSN 0021-9193. PMC 1151763. PMID 15968078.
- ^ a b Bordel, Sergio; Crombie, Andrew T.; Muñoz, Raúl; Murrell, J. Colin (2020-07-16). "Genome Scale Metabolic Model of the versatile methanotroph Methylocella silvestris". Microbial Cell Factories. 19 (1): 144. doi:10.1186/s12934-020-01395-0. ISSN 1475-2859. PMC 7364539. PMID 32677952.
Further reading
edit- Vos PW, Brenner DJ, Staley JT, Boone DR, Garrity GM, Goodfellow M, Krieg NR, Rainey FA, Schleifer KH (2005). Bergeys Manual? of Systematic Bacteriology Volume Two The Proteobacteria Part C The Alpha-, Beta-, Delta-, and Epsilonproteobacteria. Boston, MA: Bergeys Manual Trust. ISBN 0-387-29298-5.
- Dedysh SN, Knief C, Dunfield PF (July 2005). "Methylocella species are facultatively methanotrophic". Journal of Bacteriology. 187 (13): 4665–70. doi:10.1128/JB.187.13.4665-4670.2005. PMC 1151763. PMID 15968078.
- Rahman MT, Crombie A, Moussard H, Chen Y, Murrell JC (June 2011). "Acetate repression of methane oxidation by supplemental Methylocella silvestris in a peat soil microcosm". Applied and Environmental Microbiology. 77 (12): 4234–6. doi:10.1128/AEM.02902-10. PMC 3131658. PMID 21515721.
- Theisen AR, Ali MH, Radajewski S, Dumont MG, Dunfield PF, McDonald IR, et al. (November 2005). "Regulation of methane oxidation in the facultative methanotroph Methylocella silvestris BL2". Molecular Microbiology. 58 (3): 682–92. doi:10.1111/j.1365-2958.2005.04861.x. PMID 16238619. S2CID 32124521.
- Chen Y, Crombie A, Rahman MT, Dedysh SN, Liesack W, Stott MB, et al. (July 2010). "Complete genome sequence of the aerobic facultative methanotroph Methylocella silvestris BL2". Journal of Bacteriology. 192 (14): 3840–1. doi:10.1128/JB.00506-10. PMC 2897342. PMID 20472789.