The micF RNA is a non-coding RNA stress response gene found in Escherichia coli and related bacteria that post-transcriptionally controls expression of the outer membrane porin gene ompF.[1] The micF gene encodes a non-translated 93 nucleotide antisense RNA that binds its target ompF mRNA and regulates ompF expression by inhibiting translation and inducing degradation of the message. In addition, other factors, such as the RNA chaperone protein StpA also play a role in this regulatory system. The expression of micF is controlled by both environmental and internal stress factors. Four transcriptional regulators are known to bind the micF promoter region and activate micF expression.
| MicF RNA | |
|---|---|
 Predicted secondary structure and sequence conservation of MicF | |
| Identifiers | |
| Symbol | MicF |
| Rfam | RF00033 |
| Other data | |
| RNA type | Gene; antisense |
| Domain(s) | Bacteria |
| GO | GO:0006950 GO:0003729 |
| SO | SO:0000383 |
| PDB structures | PDBe |
Species distribution
editHomologues of MicF RNA have been characterised by Southern blotting in a variety of bacteria including Salmonella typhimurium, Klebsiella pneumoniae, and Pseudomonas aeruginosa.[2] MicF has also been identified computationally in Yersinia pestis and Yersinia enterocolitica.[3]
References
edit- ^ Delihas N, Forst S (October 2001). "MicF: an antisense RNA gene involved in response of Escherichia coli to global stress factors". Journal of Molecular Biology. 313 (1): 1–12. doi:10.1006/jmbi.2001.5029. PMID 11601842.
- ^ Esterling L, Delihas N (May 1994). "The regulatory RNA gene micF is present in several species of gram-negative bacteria and is phylogenetically conserved". Molecular Microbiology. 12 (4): 639–646. doi:10.1111/j.1365-2958.1994.tb01051.x. PMID 7523827. S2CID 24389234.
- ^ Delihas N (June 2003). "Annotation and evolutionary relationships of a small regulatory RNA gene micF and its target ompF in Yersinia species". BMC Microbiology. 3: 13. doi:10.1186/1471-2180-3-13. PMC 166144. PMID 12834539.
External links
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