Wikipedia

Mikael Kubista

Mikael Kubista (born 13 August 1961) is Czech-born Swedish chemist and entrepreneur who works in the field of molecular diagnostics. Since 2007, he is serving as a Professor of Chemistry and Head of the Department of Gene Expression Profiling at the Biotechnology Institute, Czech Academy of Sciences in the Czech Republic.

Prof. Mikael Kubista
Born (1961-08-13) 13 August 1961 (age 63)
Podbořany, Czechoslovakia
NationalitySwedish
CitizenshipSwedish
Alma mater
Known for
  • quantitative real-time PCR (qPCR)
  • TAATA Bio-center
Awards
  • In 2012, Pioneer of the year in western Sweden
  • In 1996, won Innovation Cup in western Sweden for the LightUp probes
Scientific career
FieldsBiochemistry
Institutions

Kubista has contributed to the field of quantitative real-time PCR (qPCR), with his work recognized as part of the early research in this area.[1][2][3][dubious – discuss]

Kubista was a member of the research team at Astra Hässle, where they focused on investigating Omeprazole, an inhibitor of K+/H+-ATPase. The drug is now marketed under the trade names Losec and Nexium, widely prescribed medications for the treatment of gastric ulcer.[4][dubious – discuss] Additionally, Kubista is the Chairman of the Board of MultiD Analyses AB and the founder of TATAA Biocenter.[5][6][7][8]

Early life

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Kubista was born to his medical doctor father in the former Czechoslovakia in 1961. His father received a scholarship and relocated to Sweden. At the age of 7 in 1968, Kubista went to Sweden to visit his father. However, on that very day, Russia invaded Czechoslovakia in the so called Warsaw Pact invasion of Czechoslovakia, and as a result, the family decided to stay making Sweden their new home.[9]

Education

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He completed his undergraduate studies at University of Gothenburg, earning a B.Sc. degree in chemistry in 1984.[10] He then pursued a Licentiate in Physical Chemistry at the Institute of Chemistry and Chemical Engineering, Chalmers University of Technology in Göteborg, which he completed in 1986.[11] Kubista obtained his Ph.D. in chemistry from Chalmers University of Technology.[12] Following his doctoral studies, he conducted postdoctoral research at institutions such as La Trobe University in Melbourne, Australia, and Yale University in New Haven, US.[8][4][dubious – discuss] Additionally, he has held visiting professor positions at various universities, including the University of Maryland in College Park, US, in June 2000, and the University of A Coruña in Spain, during September–November 2003 and July 2006 to June 2007.[citation needed] Since 2007, Kubista is serving as an adjunct professor at the Institute of Biotechnology, Czech Academy of Sciences.[13][14][dubious – discuss][9]

Career

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Academic career

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Kubista began his academic career in 1991 as an Assistant Professor in the Department of Physical Chemistry at Chalmers University of Technology.[citation needed] From 1993 to 1997, he served as an Associate Professor in the Department of Biochemistry at the same institution. Following this, he held the position of Professor in the Department of Biochemistry at Chalmers University of Technology from 1997 to 2006.[citation needed] Since 2007, he is the Head of the Department of Gene Expression at the Institute of Biotechnology, BIOCEV, Czech Academy of Sciences.[15]

Entrepreneurial activities

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TATAA Biocenter starting to give the first COVID Tests

In 1998 Kubista founded LightUp Technologies AB after his research finding of lightUp probes,[2] a company that specializes in the development of real-time PCR tests for human infectious diseases.[16][5] Three years later In 2001, Kubista's research led to the establishments of MultiD Analyses AB, which develops GenEx software for gene expression data analysis and TATAA Biocenter for qPCR and gene expression analysis.[16][5] The company became known for its qPCR training services globally and its provision of qPCR services, particularly in Europe. TATAA Biocenter was the first laboratory in Europe to obtain flexible ISO 17025 accreditation and also was the first to provide COVID tests at the onset of the pandemic.[17] In 2014 Kubista implemented non-invasive prenatal testing (NIPT) in Sweden and subsequently founded Life Genomics AB.[5] In 2020, Kubista co-founded SimSen Diagnostics, a company focused on developing technology for liquid biopsy analyses.[8][4] [18][6]

Advisorial roles and memberships

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Kubista holds several positions and advisory roles within the scientific and biotechnology communities including: Roche, ThermoFisher, Qiagen, Bio-Rad, and RealSeq Biosciences. He is also a member of the Scientific Advisory Council of Genetic Engineering News.[19][1]

Kubista has also been involved in the establishment of modern molecular diagnostics in developing countries. Since 1999, he has served as an advisor to UNESCO, providing guidance and assistance to countries such as: Libya, Egypt, Iran, Grenada, and Ghana.[4][9]

Kubista is an expert advisor for the European Commission Research Directorate General. Kubista advises the United Nations Educational Scientific and Cultural Organization (UNESCO) and is part of the scientific advisory board for the International Biotechnology Research in Tripoli, Libya, under UNESCO.[9][20][21]

Selected findings and publications

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Two-tailed schematic
  • Studied and identified chromophores and a variety of dyes commonly used as biomolecule labels like: tryptophan, DAPI, fluorescein,[22] thiazole orange, and BEBO.[23][2]
  • Explained DNA strand exchange in homologous recombination.[24]
  • Applying Widlund experiment, identified specific nucleosome positioning sequences.[16][24]
  • Uncovered mechanism of oncogene activation involving the formation of internal G-quadruplexes.[25][4][24]
  • Designed a probe that exhibit luminescence upon binding to specific nucleic acids.[26][27]
  • Techniques for gene expression at the level of individual cells and subcellular compartments.[28][24]
  • The occurrence of horizontal transfer of mitochondria within living organisms.[24][29]

Awards and recognition

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Mikael Kubista receiving the Frost and Sulvian Award
  • Was recognized by ScholarGPS as one of the 50 highly ranked scholars of 2022.[30]
  • In 2021 Kubista's organization, TATAA was on Sweden Technology Fast 50 list[6]
  • In 2019, Global Health & Pharma recognized and awarded TATAA as the "Best Nucleic Acid Analysis Service Provider – Europe."[5][20]
  • In 2013 TATAA Biocenter was honored with the Frost & Sullivan Award for Customer Value Leadership for their outstanding services in analyzing genetic material [31][20]
  • In 2012, Pioneer of the year in western Sweden[20]
  • In 1996, won Innovation Cup in western Sweden for the LightUp probes[20]


References

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  1. ^ a b "Bringing qPCR testing into the 21st century: an interview with Mikael Kubista". thermofisher. 18 October 2019. Retrieved 28 December 2023.
  2. ^ a b c "Biomolecular Detection and Quantification" (PDF). gene-quantification. Retrieved 2 January 2024.
  3. ^ "Gene Core". ibt. Retrieved 22 February 2024.
  4. ^ a b c d e "M. Kubista, PhD – Editorial Board – Molecular Aspects of Medicine – Journal – Elsevier". journals.elsevier.com. Retrieved 2023-12-28.
  5. ^ a b c d e "Mikael Kubista". BioTech Pharma Summit. Retrieved 2023-12-28.
  6. ^ a b c "TATAA Biocenter – ett av Sveriges snabbast växande teknikföretag". Mynewsdesk (in Swedish). 2021-11-19. Retrieved 2023-12-28.
  7. ^ "What's next for Gothenburg life science profile, Mikael Kubista?". sahlgrenskasciencepark.se. Retrieved 2023-12-28.
  8. ^ a b c "mikael-kubista". qiagen.com. Retrieved 2023-12-28.
  9. ^ a b c d Tachibana, Chris. "Profile, Mikael Kubista" (PDF). squarespace. Retrieved 2 January 2024.
  10. ^ "GOTEBORGS UNIVERSITET". labgenexp9. Retrieved 8 March 2024.
  11. ^ "GOTEBORGS uUNIVERSITET". labgenexp. Retrieved 8 March 2024.
  12. ^ "CHALMERS TEKNISKA HOGSCOLA". labgenexp. Retrieved 8 March 2024.
  13. ^ "Academy of Europe: CV". ae-info.org. Retrieved 2023-12-28.
  14. ^ "Scientific Advisory Board". Technology Networks. Retrieved 2023-12-28.
  15. ^ "Laboratory of Gene Expression". ibt. Retrieved 8 March 2024.
  16. ^ a b c "Mikael Kubista". publicationslist. Retrieved 28 December 2023.
  17. ^ "TATAA Biocenter to offer GLP services to developers of cell and gene therapies across the world". naringsliv. Retrieved 28 December 2023.
  18. ^ "Who we are". simsendiagnostics. Retrieved 8 March 2024.
  19. ^ "RealSeq: Mikael Kubista". 360dx. Retrieved 28 December 2023.
  20. ^ a b c d e "Academy of Europe: Kubista Mikael". ae-info.org. Retrieved 2023-12-28.
  21. ^ Göransson, Av Anders (2024-01-31). "Failed to read the fine print – lost his life's work". Life Science Sweden. Retrieved 2024-05-27.
  22. ^ Sjöback, Robert; Nygren, Jan; Kubista, Mikael (1995). "Absorption and fluorescence properties of fluorescein". Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy. 51 (6): L7–L21. Bibcode:1995AcSpA..51L...7S. doi:10.1016/0584-8539(95)01421-P. Retrieved 2 January 2024.
  23. ^ "Absorption and fluorescence properties of fluorescein". scholar.google.com. Retrieved 2023-12-28.
  24. ^ a b c d e danielduran.cz, Design: Martin Kolerus, Code: Daniel Duran. "prof. Dr. Mikael Kubista, Ph.D." biocev.eu. Biocev. Retrieved 2023-12-28.{{cite web}}: CS1 maint: multiple names: authors list (link)
  25. ^ "DNA tetraplex formation in the control region of c-my". academic. Retrieved 2 January 2024.
  26. ^ "Light-up probes: thiazole orange-conjugated peptide nucleic acid for detection of target nucleic acid in homogeneous solution". scholar.google.com. Retrieved 2023-12-28.
  27. ^ Svanvik, Nicke; Westman, Gunnar; Wang, Dongyuan; Kubista, Mikael (2000). "Light-Up Probes: Thiazole Orange-Conjugated Peptide Nucleic Acid for Detection of Target Nucleic Acid in Homogeneous Solution". Analytical Biochemistry. 281 (1): 26–35. doi:10.1006/abio.2000.4534. PMID 10847607. Retrieved 2 January 2024.
  28. ^ Bengtsson, M.; Ståhlberg, A.; Rorsman, P.; Kubista, M. (2005). "Gene expression profiling in single cells from the pancreatic islets of Langerhans reveals lognormal distribution of mRNA levels". Genome Research. 15 (10): 1388–1392. doi:10.1101/gr.3820805. PMC 1240081. PMID 16204192. Retrieved 2 January 2024.
  29. ^ "Horizontal transfer of whole mitochondria restores tumorigenic potential in mitochondrial DNA-deficient cancer cells". scholar.google.com. Retrieved 2023-12-28.
  30. ^ "Highly Ranked Scholars™ 2022". scholargps.com. Retrieved 27 May 2024.
  31. ^ "ORCID". orcid.org. Retrieved 2023-12-28.
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