Multisite-specific tRNA:(cytosine-C5)-methyltransferase (EC 2.1.1.202, multisite-specific tRNA:m5C-methyltransferase, TRM4 (gene)) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytosine-C5)-methyltransferase.[1][2][3][4] This enzyme catalyses the following chemical reaction
| Multisite-specific tRNA:(cytosine-C5)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.202 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- (1) S-adenosyl-L-methionine + cytosine34 in tRNA precursor S-adenosyl-L-homocysteine + 5-methylcytosine34 in tRNA precursor
- (2) S-adenosyl-L-methionine + cytosine40 in tRNA precursor S-adenosyl-L-homocysteine + 5-methylcytosine40 in tRNA precursor
- (3) S-adenosyl-L-methionine + cytosine48 in tRNA S-adenosyl-L-homocysteine + 5-methylcytosine48 in tRNA
- (4) S-adenosyl-L-methionine + cytosine49 in tRNA S-adenosyl-L-homocysteine + 5-methylcytosine49 in tRNA
The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA.
References
edit- ^ Motorin Y, Grosjean H (August 1999). "Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme". RNA. 5 (8): 1105–18. doi:10.1017/s1355838299982201. PMC 1369833. PMID 10445884.
- ^ Jiang HQ, Motorin Y, Jin YX, Grosjean H (July 1997). "Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study". Nucleic Acids Research. 25 (14): 2694–701. doi:10.1093/nar/25.14.2694. PMC 146816. PMID 9207014.
- ^ Strobel MC, Abelson J (July 1986). "Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo". Molecular and Cellular Biology. 6 (7): 2663–73. doi:10.1128/mcb.6.7.2663. PMC 367823. PMID 3537724.
- ^ Walbott H, Husson C, Auxilien S, Golinelli-Pimpaneau B (July 2007). "Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m5C methyltransferase". RNA. 13 (7): 967–73. doi:10.1261/rna.515707. PMC 1894932. PMID 17475914.
External links
edit- Multisite-specific+tRNA:(cytosine-C5)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
