N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (EC 3.5.1.103, MshB) is an enzyme with systematic name 1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol acetylhydrolase.[1][2][3] This enzyme catalyses the following chemical reaction
| N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.5.1.103 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- 1-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol + H2O 1-(2-amino-2-deoxy-alpha-D-glucopyranoside)-1D-myo-inositol + acetate
This enzyme mediates the rate limiting step in the mycothiol biosynthesis pathway.
References
edit- ^ Rawat M, Kovacevic S, Billman-Jacobe H, Av-Gay Y (May 2003). "Inactivation of mshB, a key gene in the mycothiol biosynthesis pathway in Mycobacterium smegmatis". Microbiology. 149 (Pt 5): 1341–9. doi:10.1099/mic.0.26084-0. PMID 12724395.
- ^ Newton GL, Av-Gay Y, Fahey RC (December 2000). "N-Acetyl-1-D-myo-inosityl-2-amino-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) is a key enzyme in mycothiol biosynthesis". Journal of Bacteriology. 182 (24): 6958–63. doi:10.1128/jb.182.24.6958-6963.2000. PMC 94821. PMID 11092856.
- ^ Maynes JT, Garen C, Cherney MM, Newton G, Arad D, Av-Gay Y, Fahey RC, James MN (November 2003). "The crystal structure of 1-D-myo-inosityl 2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase (MshB) from Mycobacterium tuberculosis reveals a zinc hydrolase with a lactate dehydrogenase fold". The Journal of Biological Chemistry. 278 (47): 47166–70. doi:10.1074/jbc.m308914200. PMID 12958317.
External links
edit- N-acetyl-1-D-myo-inositol-2-amino-2-deoxy-alpha-D-glucopyranoside+deacetylase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
