N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase (EC 2.4.1.289, WbbL) is an enzyme with systematic name dTDP-6-deoxy-beta-L-mannose:N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol 3-alpha-L-rhamnosyltransferase.[1][2] This enzyme catalyses the following chemical reaction
N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase | |||||||||
---|---|---|---|---|---|---|---|---|---|
Identifiers | |||||||||
EC no. | 2.4.1.289 | ||||||||
Databases | |||||||||
IntEnz | IntEnz view | ||||||||
BRENDA | BRENDA entry | ||||||||
ExPASy | NiceZyme view | ||||||||
KEGG | KEGG entry | ||||||||
MetaCyc | metabolic pathway | ||||||||
PRIAM | profile | ||||||||
PDB structures | RCSB PDB PDBe PDBsum | ||||||||
|
- dTDP-6-deoxy-beta-L-mannose + N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol dTDP + alpha-L-rhamnopyranosyl-(1->3)-N-acetyl-alpha-D-glucosaminyl-diphospho-trans,octacis-decaprenol
This enzyme requires Mn2+ or Mg2+.
References
edit- ^ Mills JA, Motichka K, Jucker M, Wu HP, Uhlik BC, Stern RJ, Scherman MS, Vissa VD, Pan F, Kundu M, Ma YF, McNeil M (October 2004). "Inactivation of the mycobacterial rhamnosyltransferase, which is needed for the formation of the arabinogalactan-peptidoglycan linker, leads to irreversible loss of viability". The Journal of Biological Chemistry. 279 (42): 43540–6. doi:10.1074/jbc.M407782200. PMID 15294902.
- ^ Grzegorzewicz AE, Ma Y, Jones V, Crick D, Liav A, McNeil MR (December 2008). "Development of a microtitre plate-based assay for lipid-linked glycosyltransferase products using the mycobacterial cell wall rhamnosyltransferase WbbL". Microbiology. 154 (Pt 12): 3724–30. doi:10.1099/mic.0.2008/023366-0. PMC 2717732. PMID 19047740.
External links
edit- N-acetylglucosaminyl-diphospho-decaprenol+L-rhamnosyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)