N1-acetylpolyamine oxidase (EC 1.5.3.13, hPAO-1, mPAO, hPAO) is an enzyme with systematic name N1-acetylpolyamine:oxygen oxidoreductase (3-acetamidopropanal-forming).[1][2][3][4] This enzyme catalyses the following chemical reaction
| N1-acetylpolyamine oxidase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.5.3.13 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- (1) N1-acetylspermidine + O2 + H2O putrescine + 3-acetamidopropanal + H2O2
- (2) N1-acetylspermine + O2 + H2O spermidine + 3-acetamidopropanal + H2O2
This enzyme also catalyses the reaction:
- N1,N12-diacetylspermine + O2 + H2O N1-acetylspermidine + 3-acetamamidopropanal + H2O2.
References
edit- ^ Vujcic S, Liang P, Diegelman P, Kramer DL, Porter CW (February 2003). "Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion". The Biochemical Journal. 370 (Pt 1): 19–28. doi:10.1042/BJ20021779. PMC 1223169. PMID 12477380.
- ^ Järvinen A, Grigorenko N, Khomutov AR, Hyvönen MT, Uimari A, Vepsäläinen J, Sinervirta R, Keinänen TA, Vujcic S, Alhonen L, Porter CW, Jänne J (February 2005). "Metabolic stability of alpha-methylated polyamine derivatives and their use as substitutes for the natural polyamines". The Journal of Biological Chemistry. 280 (8): 6595–601. doi:10.1074/jbc.M412788200. PMID 15611107.
- ^ Wang Y, Hacker A, Murray-Stewart T, Frydman B, Valasinas A, Fraser AV, Woster PM, Casero RA (July 2005). "Properties of recombinant human N1-acetylpolyamine oxidase (hPAO): potential role in determining drug sensitivity". Cancer Chemotherapy and Pharmacology. 56 (1): 83–90. doi:10.1007/s00280-004-0936-5. PMID 15791459.
- ^ Wu T, Yankovskaya V, McIntire WS (June 2003). "Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N1-acetylated polyamine oxidase". The Journal of Biological Chemistry. 278 (23): 20514–25. doi:10.1074/jbc.M302149200. PMID 12660232.
External links
edit- N1-acetylpolyamine+oxidase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
