NUDT21

Cleavage and polyadenylation specificity factor subunit 5 (CPSF5) is an enzyme that in humans is encoded by the NUDT21 gene. It belongs to the Nudix family of hydrolases.

NUDT21
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2CL3, 2J8Q, 3BAP, 3BHO, 3MDG, 3MDI, 3N9U, 3P5T, 3P6Y, 3Q2S, 3Q2T
Identifiers
Aliases	NUDT21, CFIM25, CPSF5, nudix hydrolase 21
External IDs	OMIM: 604978; MGI: 1915469; HomoloGene: 5090; GeneCards: NUDT21; OMA:NUDT21 - orthologs
Gene location (Human) Chr. Chromosome 16 (human) Band 16q13 Start 56,429,133 bp End 56,452,199 bp
Gene location (Mouse) Chr. Chromosome 8 (mouse) Band 8|8 C5 Start 94,742,124 bp End 94,763,659 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in gonad ganglionic eminence ventricular zone pons caput epididymis left testis germinal epithelium superior vestibular nucleus Achilles tendon right testis Top expressed in superior cervical ganglion spermatocyte ventricular zone spermatid embryo yolk sac hand embryo ganglionic eminence tail of embryo More reference expression data BioGPS More reference expression data
Gene ontology Molecular function protein homodimerization activity histone deacetylase binding protein binding mRNA binding hydrolase activity RNA binding identical protein binding chromatin binding mRNA 3'-UTR AU-rich region binding Cellular component paraspeckles centrosome nucleoplasm microtubule organizing center mRNA cleavage factor complex nucleus nuclear body cytoplasm mRNA cleavage and polyadenylation specificity factor complex Biological process mRNA splicing, via spliceosome termination of RNA polymerase II transcription protein tetramerization mRNA 3'-end processing mRNA polyadenylation mRNA processing posttranscriptional regulation of gene expression cell differentiation positive regulation of mRNA cleavage protein heterotetramerization pre-mRNA cleavage required for polyadenylation mRNA alternative polyadenylation positive regulation of mRNA polyadenylation messenger ribonucleoprotein complex assembly positive regulation of stem cell differentiation positive regulation of pro-B cell differentiation Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 11051 68219 Ensembl ENSG00000167005 ENSMUSG00000031754 UniProt O43809 Q9CQF3 RefSeq (mRNA) NM_007006 NM_026623 RefSeq (protein) NP_008937 NP_080899 Location (UCSC) Chr 16: 56.43 – 56.45 Mb Chr 8: 94.74 – 94.76 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

The protein encoded by this gene is one subunit of the cleavage factor Im complex required for 3' RNA cleavage and polyadenylation processing. The interaction of the protein with the RNA is one of the earliest steps in the assembly of the 3' end processing complex and facilitates the recruitment of other processing factors. This gene encodes the 25kD subunit of the protein complex, which is composed of four polypeptides.

Further reading

• Rüegsegger U, Beyer K, Keller W (1996). "Purification and characterization of human cleavage factor Im involved in the 3' end processing of messenger RNA precursors". J. Biol. Chem. 271 (11): 6107–13. doi:10.1074/jbc.271.11.6107. PMID 8626397.
• de Vries H, Rüegsegger U, Hübner W, et al. (2000). "Human pre-mRNA cleavage factor IIm contains homologs of yeast proteins and bridges two other cleavage factors". EMBO J. 19 (21): 5895–904. doi:10.1093/emboj/19.21.5895. PMC 305781. PMID 11060040.
• Hartley JL, Temple GF, Brasch MA (2001). "DNA Cloning Using In Vitro Site-Specific Recombination". Genome Res. 10 (11): 1788–95. doi:10.1101/gr.143000. PMC 310948. PMID 11076863.
• Wiemann S, Weil B, Wellenreuther R, et al. (2001). "Toward a Catalog of Human Genes and Proteins: Sequencing and Analysis of 500 Novel Complete Protein Coding Human cDNAs". Genome Res. 11 (3): 422–35. doi:10.1101/gr.GR1547R. PMC 311072. PMID 11230166.
• Simpson JC, Wellenreuther R, Poustka A, et al. (2001). "Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing". EMBO Rep. 1 (3): 287–92. doi:10.1093/embo-reports/kvd058. PMC 1083732. PMID 11256614.
• Kim H, Lee Y (2002). "Interaction of poly(A) polymerase with the 25-kDa subunit of cleavage factor I". Biochem. Biophys. Res. Commun. 289 (2): 513–8. doi:10.1006/bbrc.2001.5992. PMID 11716503.
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. Bibcode:2002PNAS...9916899M. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
• Awasthi S, Alwine JC (2003). "Association of polyadenylation cleavage factor I with U1 snRNP". RNA. 9 (11): 1400–9. doi:10.1261/rna.5104603. PMC 1287061. PMID 14561889.
• Brown KM, Gilmartin GM (2004). "A mechanism for the regulation of pre-mRNA 3' processing by human cleavage factor Im". Mol. Cell. 12 (6): 1467–76. doi:10.1016/S1097-2765(03)00453-2. PMID 14690600.
• Bouwmeester T, Bauch A, Ruffner H, et al. (2004). "A physical and functional map of the human TNF-alpha/NF-kappa B signal transduction pathway". Nat. Cell Biol. 6 (2): 97–105. doi:10.1038/ncb1086. PMID 14743216. S2CID 11683986.
• Dettwiler S, Aringhieri C, Cardinale S, et al. (2005). "Distinct sequence motifs within the 68-kDa subunit of cleavage factor Im mediate RNA binding, protein-protein interactions, and subcellular localization" (PDF). J. Biol. Chem. 279 (34): 35788–97. doi:10.1074/jbc.M403927200. PMID 15169763. S2CID 39752996.
• Lehner B, Sanderson CM (2004). "A Protein Interaction Framework for Human mRNA Degradation". Genome Res. 14 (7): 1315–23. doi:10.1101/gr.2122004. PMC 442147. PMID 15231747.
• Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
• Wiemann S, Arlt D, Huber W, et al. (2004). "From ORFeome to Biology: A Functional Genomics Pipeline". Genome Res. 14 (10B): 2136–44. doi:10.1101/gr.2576704. PMC 528930. PMID 15489336.
• Rush J, Moritz A, Lee KA, et al. (2005). "Immunoaffinity profiling of tyrosine phosphorylation in cancer cells". Nat. Biotechnol. 23 (1): 94–101. doi:10.1038/nbt1046. PMID 15592455. S2CID 7200157.
• Andersen JS, Lam YW, Leung AK, et al. (2005). "Nucleolar proteome dynamics". Nature. 433 (7021): 77–83. Bibcode:2005Natur.433...77A. doi:10.1038/nature03207. PMID 15635413. S2CID 4344740.
• Stelzl U, Worm U, Lalowski M, et al. (2005). "A human protein-protein interaction network: a resource for annotating the proteome". Cell. 122 (6): 957–68. doi:10.1016/j.cell.2005.08.029. hdl:11858/00-001M-0000-0010-8592-0. PMID 16169070. S2CID 8235923.
• Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. Bibcode:2005Natur.437.1173R. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
• Mehrle A, Rosenfelder H, Schupp I, et al. (2006). "The LIFEdb database in 2006". Nucleic Acids Res. 34 (Database issue): D415–8. doi:10.1093/nar/gkj139. PMC 1347501. PMID 16381901.